Sec23b (NM_001281816) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228477 representing NM_001281816 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGAGTCTCCTTCCTCCAGATGCCCTGGTTGGTCTCATCACTTTTGGGAGGATGGTGCAGGTTC ATGAGCTAAGCTGTGAAGGAATCTCCAAGAGCTATGTCTTCCGAGGGACCAAGGATTTAACTGCAAAGCA AATACAGGAGATGCTGGGCCTGACCAAGTCAGCCATGCCTGTGCAGCAAGCCAGACCTGCTCAACCTCAG GAGCAGCCTTTTGTTTCCAGCAGATTTCTACAGCCCATTCACAAGATTGACATGAACCTCACAGATCTTC TCGGGGAGCTGCAGAGGGACCCATGGCCAGTAACTCAGGGGAAGAGACCACTGCGGTCCACTGGTGTTGC TTTGTCCATTGCTGTTGGCTTGTTGGAGGGGACTTTCCCAAATACTGGAGCCAGGATCATGCTGTTCACT GGCGGGCCCCCGACCCAAGGACCTGGCATGGTGGTTGGAGATGAGTTGAAGACTCCTATTCGTTCCTGGC ACGACATTGAGAAAGATAACGCACGATTCATGAAGAAGGCAACCAAGCATTATGAGATGCTTGCTAATCG AACTGCTACAAATGGCCACTGCATTGATATTTATGCTTGTGCCCTTGACCAAACTGGACTTCTGGAGATG AAGTGCTGTCCAAATCTTACAGGAGGCCACATGGTGATGGGAGATTCTTTCAACACTTCTCTTTTCAAGC AGACATTCCAAAGAATTTTTAGTAAAGACTTCAACGGAGATTTCAGAATGGCGTTTGGTGCTACGTTGGA TGTAAAGACCTCTCGGGAACTGAAGATTGCAGGAGCCATTGGTCCATGCGTGTCTCTGAATGTGAAAGGG CCATGCGTGTCAGAAAATGAGCTTGGTGTTGGTGGCACAAGTCAGTGGAAGATCTGTGGCCTGGATCCCT CATCTACTCTTGGCATCTACTTTGAAGTTGTCAATCAGCACAATGCTCCAGTTCCCCAGGGAGGCAGAGG TGCCATCCAGTTTGTCACACAGTATCAGCATTCCAGCACTCAGAAGCGCATCCGAGTGACCACCATTGCC CGCAATTGGGCAGATGCACAGAGTCAGCTGAGGCACATAGAAGCAGCATTTGACCAGGAGGCCGCAGCAG TCCTGATGGCACGGCTTGGAGTGTTCCGCGCAGAGTCAGAGGAGGGGCCTGACGTGCTTCGGTGGCTGGA TCGGCAACTCATCAGACTGTGTCAGAAGTTTGGGCAGTATAACAAAGAAGACCCTACGTCTTTCAGATTG TCAGACTCCTTTTCTCTGTATCCTCAGTTCATGTTCCATCTGAGAAGATCTCCATTTCTTCAAGTATTTA ACAACAGTCCTGATGAGTCCTCCTATTATAGACACCATTTTGCCCGGCAGGATCTGACTCAGTCCCTCAT TATGATCCAGCCCATTCTCTATTCTTACTCCTTCCACGGACCACCAGAGCCTGTACTCCTGGACAGCAGT AGCATTCTAGCTGACAGAATTCTGCTGATGGATACTTTCTTCCAAATTGTCATTTACCTTGGTGAGACCA TAGCCCAGTGGCGGAAGGCTGGATACCAGGACATGCCCGAGTATGAAAACTTCAAGCACCTTCTTCAGGC GCCCCTGGATGATGCCCAAGAAATTCTGCAAGCACGCTTCCCAATGCCACGTTATATCAACACAGAGCAT GGCGGCAGTCAGGCTCGGTTCCTGTTGTCTAAAGTTAACCCATCTCAGACACACAATAACCTGTATGCCT GGGGGCAGGAAACTGGAGCGCCCATCCTGACAGACGATGTCAGCCTACAGGTGTTCATGGATCACCTGAA GAAGCTGGCTGTGTCTAGTGCCTCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281816
Insert Size	1848 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281816.1, NP_001268745.1
RefSeq Size	2421 bp
RefSeq ORF	1848 bp
Locus ID	27054
Gene Summary	Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 1. This results in an isoform (2) with a shorter N-terminus compared to isoform 1.

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