Cacnb2 (NM_001252533) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW060387; CAB2; Cavbeta2; Cchb2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230660 representing NM_001252533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGACAGGCAGTTGGTGTCTTCTCAGACTCAATCCAGTATTCCTGGGGGTTCGGCAGACTCCTACA CCAGCCGCCCATCCGATTCAGATGTGTCTTTGGAAGAGGACCGGGAGGCAGTACGTAGAGAAGCTGAGCG GCAGGCACAGGCACAGTTGGAAAAAGCAAAGACAAAACCTGTTGCATTTGCAGTTCGGACCAATGTCAGA TACAGCGCAGCCCAGGAGGATGACGTTCCGGTGCCTGGCATGGCCATCTCCTTCGAGGCCAAAGATTTTC TGCATGTTAAAGAAAAATTTAATAATGACTGGTGGATAGGACGGCTGGTTAAAGAAGGCTGTGAAATCGG ATTTATTCCAAGCCCAGTCAAACTAGAGAACATGAGGCTACAGCACGAACAGAGAGCCAAGCAAGGGAAA TTCTATTCCAGTAAATCAGGAGGGAATTCATCATCCAGTTTGGGTGACATAGTGCCCAGTTCCAGAAAAT CGACACCGCCGTCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAAGAGAATGATATTCCAGC AAACCACCGCTCCCCTAAGCCCAGTGCAAACAGTGTAACATCACCCCACTCCAAAGAGAAAAGAATGCCC TTCTTTAAGAAGACAGAGCACACTCCTCCTTATGATGTGGTACCTTCCATGCGACCAGTGGTGCTGGTGG GCCCGTCTCTGAAGGGGTATGAGGTCACAGATATGATGCAAAAAGCACTGTTTGATTTTCTAAAACACAG ATTTGAAGGGCGGATATCCATCACAAGAGTCACTGCTGACATCTCCCTTGCCAAACGCTCAGTATTAAAC AATCCCAGTAAGCATGCAATCATAGAAAGATCCAACACAAGGTCCAGCTTAGCGGAAGTTCAGAGTGAAA TTGAAAGGATTTTTGAACTTGCAAGAACACTGCAATTGGTAGTCCTTGACGCGGATACAATTAATCACCC AGCTCAACTCAGTAAGACCTCTTTGGCCCCTATCATAGTGTACGTAAAGATTTCTTCTCCCAAGGTTTTA CAAAGGTTGATAAAATCTCGAGGGAAATCTCAAGCAAAACACCTCAATGTCCAGATGGTAGCAGCTGATA AACTGGCCCAATGTCCTCCGCAGGAATCATTTGATGTGATCTTGGATGAGAACCAGCTGGAGGACGCTTG TGAGCATCTTGCCGACTATCTGGAGGCATACTGGAAGGCCACCCACCCTCCCAGCGGTAACCTCCCCAAC CCTCTGCTTAGCCGGACTTTAGCCTCCTCAACTTTACCTCTGAGCCCCACCCTCGCCTCTAATTCACAGG GTTCTCAAGGTGATCAAAGGCCTGATCGCTCTGCTCCCCGGTCTGCTTCCCAAGCTGAAGAAGAACCTTG CCTAGAACCCGTCAAAAAATCCCAACACCGTTCCTCCTCAGCCACACACCAAAACCACCGCAGCGGGACA GGTCGAGGCCTCTCTAGGCAAGAGACGTTTGACTCTGAAACCCAAGAGAGCCGAGACTCTGCCTACGTGG AGCCAAAGGAAGATTATTCACATGAACATGTGGACCGGTATGTCCCACACCGCGAGCATAACCACAGAGA GGAGACCCACAGCAGCAATGGCCACAGACACAGGGAGTCTCGCCACCGCTCTAGGGACATGGGTCGAGAC CAGGACCACAATGAGTGCATCAAACAACGAAGCCGGCATAAATCTAAGGATCGCTACTGTGACAAGGAGG GGGAAGTAATATCCAAGAGAAGGAATGAGGCTGGCGAGTGGAACAGGGATGTATACATCCGCCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230660 representing NM_001252533 Red=Cloning site Green=Tags(s) MLDRQLVSSQTQSSIPGGSADSYTSRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVR YSAAQEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSPVKLENMRLQHEQRAKQGK FYSSKSGGNSSSSLGDIVPSSRKSTPPSSAIDIDATGLDAEENDIPANHRSPKPSANSVTSPHSKEKRMP FFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLN NPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIIVYVKISSPKVL QRLIKSRGKSQAKHLNVQMVAADKLAQCPPQESFDVILDENQLEDACEHLADYLEAYWKATHPPSGNLPN PLLSRTLASSTLPLSPTLASNSQGSQGDQRPDRSAPRSASQAEEEPCLEPVKKSQHRSSSATHQNHRSGT GRGLSRQETFDSETQESRDSAYVEPKEDYSHEHVDRYVPHREHNHREETHSSNGHRHRESRHRSRDMGRD QDHNECIKQRSRHKSKDRYCDKEGEVISKRRNEAGEWNRDVYIRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252533
ORF Size	1815 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252533.1, NP_001239462.1
RefSeq Size	3814 bp
RefSeq ORF	1818 bp
Locus ID	12296
MW	68.5 kDa
Gene Summary	The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function]

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