Cacnb2 (NM_001252533) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW060387; CAB2; Cavbeta2; Cchb2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228449 representing NM_001252533 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGACAGGCAGTTGGTGTCTTCTCAGACTCAATCCAGTATTCCTGGGGGTTCGGCAGACTCCTACA CCAGCCGCCCATCCGATTCAGATGTGTCTTTGGAAGAGGACCGGGAGGCAGTACGTAGAGAAGCTGAGCG GCAGGCACAGGCACAGTTGGAAAAAGCAAAGACAAAACCTGTTGCATTTGCAGTTCGGACCAATGTCAGA TACAGCGCAGCCCAGGAGGATGACGTTCCGGTGCCTGGCATGGCCATCTCCTTCGAGGCCAAAGATTTTC TGCATGTTAAAGAAAAATTTAATAATGACTGGTGGATAGGACGGCTGGTTAAAGAAGGCTGTGAAATCGG ATTTATTCCAAGCCCAGTCAAACTAGAGAACATGAGGCTACAGCACGAACAGAGAGCCAAGCAAGGGAAA TTCTATTCCAGTAAATCAGGAGGGAATTCATCATCCAGTTTGGGTGACATAGTGCCCAGTTCCAGAAAAT CGACACCGCCGTCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAAGAGAATGATATTCCAGC AAACCACCGCTCCCCTAAGCCCAGTGCAAACAGTGTAACATCACCCCACTCCAAAGAGAAAAGAATGCCC TTCTTTAAGAAGACAGAGCACACTCCTCCTTATGATGTGGTACCTTCCATGCGACCAGTGGTGCTGGTGG GCCCGTCTCTGAAGGGGTATGAGGTCACAGATATGATGCAAAAAGCACTGTTTGATTTTCTAAAACACAG ATTTGAAGGGCGGATATCCATCACAAGAGTCACTGCTGACATCTCCCTTGCCAAACGCTCAGTATTAAAC AATCCCAGTAAGCATGCAATCATAGAAAGATCCAACACAAGGTCCAGCTTAGCGGAAGTTCAGAGTGAAA TTGAAAGGATTTTTGAACTTGCAAGAACACTGCAATTGGTAGTCCTTGACGCGGATACAATTAATCACCC AGCTCAACTCAGTAAGACCTCTTTGGCCCCTATCATAGTGTACGTAAAGATTTCTTCTCCCAAGGTTTTA CAAAGGTTGATAAAATCTCGAGGGAAATCTCAAGCAAAACACCTCAATGTCCAGATGGTAGCAGCTGATA AACTGGCCCAATGTCCTCCGCAGGAATCATTTGATGTGATCTTGGATGAGAACCAGCTGGAGGACGCTTG TGAGCATCTTGCCGACTATCTGGAGGCATACTGGAAGGCCACCCACCCTCCCAGCGGTAACCTCCCCAAC CCTCTGCTTAGCCGGACTTTAGCCTCCTCAACTTTACCTCTGAGCCCCACCCTCGCCTCTAATTCACAGG GTTCTCAAGGTGATCAAAGGCCTGATCGCTCTGCTCCCCGGTCTGCTTCCCAAGCTGAAGAAGAACCTTG CCTAGAACCCGTCAAAAAATCCCAACACCGTTCCTCCTCAGCCACACACCAAAACCACCGCAGCGGGACA GGTCGAGGCCTCTCTAGGCAAGAGACGTTTGACTCTGAAACCCAAGAGAGCCGAGACTCTGCCTACGTGG AGCCAAAGGAAGATTATTCACATGAACATGTGGACCGGTATGTCCCACACCGCGAGCATAACCACAGAGA GGAGACCCACAGCAGCAATGGCCACAGACACAGGGAGTCTCGCCACCGCTCTAGGGACATGGGTCGAGAC CAGGACCACAATGAGTGCATCAAACAACGAAGCCGGCATAAATCTAAGGATCGCTACTGTGACAAGGAGG GGGAAGTAATATCCAAGAGAAGGAATGAGGCTGGCGAGTGGAACAGGGATGTATACATCCGCCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252533
Insert Size	1818 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252533.1, NP_001239462.1
RefSeq Size	3814 bp
RefSeq ORF	1818 bp
Locus ID	12296
Gene Summary	The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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