Prc1 (NM_001285997) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D7Ertd348e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230651 representing NM_001285997 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGAAGTGAGGTGCTAGCAGATGAGTCTATCACATGCCTGCAGAAAGCTCTAACTCACCTCCGGG AAATATGGGAACTAATTGGGATTCCAGAGGAGCAGAGGCTACAAAGAACCGAGGTTGTAAAGAAGCATAT AAAGGATCTTCTGGATAGAATGATTGCTGAAGAGGAGAGCCTGAGGGAAAGGCTGCTCAAAAGCATATCC ATCTGTCAGAAGGAGCTGAGTACCCTGTGCAGTGAGCTACAAGTGAAGCCATTTCAGGAAGAAAAAGATA CAACCATCTTGCAACTAGAAAAAGATTTGCGCACTCAAGTAGAATTGATGAGAAAACAGAAAAAGGAGAG AAAGCAGGAACTGAAGCTACTTCAGGAACAGGAGCAAGAACTCCGTGACATCCTCTGTATGCCGCCCTGT GATGTGGACAGCACCTCTGTCCCCACCTTAGAAGAGCTGAAGCTATTCCGACAGCGTGTGGCAACGCTGA GGGAGACAAAGGAATCGAGGCGTGAAGAATTTGTCAACATTAAGAAACAAATCATATTGTGTATGGAAGA ATTAGAACACTCTCCAGATACAAGCTTTGAAAGAGATGTGGTGTGTGAAGATGAAAGTGCTTTTTGTTTA TCACTGGAGAACATTGCAACATTACAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAG CAGAATGTGAGGGGCTCCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAG AGAGCCTGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGAT CGGTTAGAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAAT TCTGGGACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACAC AGAAAACCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGTTCACAAGGAG CTGTTCCAAGGCGTCCAGAAGTGGGAAGAGAGCTGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAG ATCCAGGTCGATTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAA AACACTCCCTAAGCTGGAAGAGGAGTTGAAAGCACGGATTGAACAGTGGGAACAGGAGCACTCAACAGCA TTTGTGGTGAATGGGCAGAAATTCATGGAGTATGTTACAGAACAGTGGGAATTACATCGGCTGGAGAAAG AGCGAGCCAAACAGGAGAGGCAACTGAAGAACAAGAAACAGACAGAGGCAGAGATGCTGTATGGCAGTAC TCCCCGGACACCTAGCAAAAGACCAGGACAGACGCCCAAGAAGTCTGGCAAAATGAACACTACCACCATG TCTAGTGCTACACCCAACAGTAGCATTCGGCCTGTCTTTGGTGGATCAGTGTACCGGTCACCTATGTCTC GACTACCCCCTTCTGGCAGCAAGTCAGTTGTCACTTCTCTGTGTTCTGGAAAGAAAACACCTCGAGCTGC CCAGCTTAGGGCCAACAAGGAGAACTTGGACCTCAATGGCAGCATCCTGAGCGGTGGGTACCCTGGCTCG ACGCCCCTCCAGCACAACTGCAGCATTAAGTCTGTTGCCAGCACCTATTCTGAGTTTTCGCGAGAACTTT CAAAGGCTTCCAGATCTGATGCTACGTCTCGAATCCTCAATTCAACCAACATCCAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230651 representing NM_001285997 Red=Cloning site Green=Tags(s) MRRSEVLADESITCLQKALTHLREIWELIGIPEEQRLQRTEVVKKHIKDLLDRMIAEEESLRERLLKSIS ICQKELSTLCSELQVKPFQEEKDTTILQLEKDLRTQVELMRKQKKERKQELKLLQEQEQELRDILCMPPC DVDSTSVPTLEELKLFRQRVATLRETKESRREEFVNIKKQIILCMEELEHSPDTSFERDVVCEDESAFCL SLENIATLQKLLKQLEMKKSQNEAECEGLRTQIRELWDRLQIPEEEREPVEAIMTGSKTKIRNALKLEVD RLEELKMQNIKQVIEKIRVELAQFWDQCFYSQEQRQAFAPYYSEDYTENLLHLHDAEIVRLRNYYDVHKE LFQGVQKWEESWKLFLEFERKASDPGRFTNRGGNLLKEEKERAKLQKTLPKLEEELKARIEQWEQEHSTA FVVNGQKFMEYVTEQWELHRLEKERAKQERQLKNKKQTEAEMLYGSTPRTPSKRPGQTPKKSGKMNTTTM SSATPNSSIRPVFGGSVYRSPMSRLPPSGSKSVVTSLCSGKKTPRAAQLRANKENLDLNGSILSGGYPGS TPLQHNCSIKSVASTYSEFSRELSKASRSDATSRILNSTNIQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285997
ORF Size	1809 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285997.1, NP_001272926.1
RefSeq Size	3040 bp
RefSeq ORF	1812 bp
Locus ID	233406
UniProt ID	Q99K43
MW	70.7 kDa
Gene Summary	Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.[UniProtKB/Swiss-Prot Function]

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