Cnot6 (NM_001290741) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CCR4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230505 representing NM_001290741 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT GTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGGTACTGCAAAAAGAA TTTCAACAGAACAACCACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGC CTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGT CCATCATGGGCACTTAATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTG ATATTATAAGTCTTCAGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACG TGGCTATAATGGATTCTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTT GATGGCTGTGCAATATTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATC AGCTAGCAATGGCGAATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGG AGTTGCAGTACTGCTAGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACA GAAAAACAACTTATTCTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGG TACAAACTATGATGTTCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAG TGTATTGGGAGAATGTGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGT GTTGTAGAGTATTTGAGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATG AAAGTCTCACAAACTTCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAA GTTAAAGAGTGCCTATGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATT GACTACATCTTCTATTCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGC TAGTCGAGAATAACATCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACA ATTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230505 representing NM_001290741 Red=Cloning site Green=Tags(s) MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSSSLWSLTHLTALHLSDNSLSCIPSDI AKLHNLVYLDLSHNQIQSLPAELGNMVSLRELHLNYNQLRVLPFELGKLFQLQTLSLKGNPLTQDILNLC LEPDGTRRLLNYLLDNLSGTAKRISTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYC PSWALNWDYRKKAIIQEILSCNADIISLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHV DGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKELIEMSSGKPHLGT EKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRSLKSSVLGECGTIPLVLCADLNSLPDSG VVEYLSTGGVETNHKDFKELRYNESLTNFSCNGKNGMTNGRITHGFKLKSAYENGLMPYTNYTFDFKGII DYIFYSKPQLNTLAILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290741
ORF Size	1671 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290741.2
RefSeq Size	5372 bp
RefSeq ORF	1674 bp
Locus ID	104625
UniProt ID	Q8K3P5
MW	63.8 kDa
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]

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