Cnot6 (NM_001290741) Mouse Untagged Clone

CAT#: MC228294

Cnot6 (untagged) - Mouse CCR4-NOT transcription complex, subunit 6 (Cnot6), transcript variant 1



  "NM_001290741" in other vectors (1)

CNY 5,420.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CCR4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228294 representing NM_001290741
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG
CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT
GTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT
GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG
GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG
AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT
CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGGTACTGCAAAAAGAA
TTTCAACAGAACAACCACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGC
CTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGT
CCATCATGGGCACTTAATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTG
ATATTATAAGTCTTCAGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACG
TGGCTATAATGGATTCTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTT
GATGGCTGTGCAATATTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATC
AGCTAGCAATGGCGAATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGG
AGTTGCAGTACTGCTAGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACA
GAAAAACAACTTATTCTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGG
TACAAACTATGATGTTCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAG
TGTATTGGGAGAATGTGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGT
GTTGTAGAGTATTTGAGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATG
AAAGTCTCACAAACTTCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAA
GTTAAAGAGTGCCTATGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATT
GACTACATCTTCTATTCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGC
TAGTCGAGAATAACATCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACA
ATTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290741
Insert Size 1674 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290741.1, NP_001277670.1
RefSeq Size 5372 bp
RefSeq ORF 1674 bp
Locus ID 104625
UniProt ID Q8K3P5
Gene Summary Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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