Cnot6 (NM_001290741) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CCR4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228294 representing NM_001290741 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAAGAAAAGTACGAGCCCCCTGACCCTCGGAGGATGTACACAATTATGTCCTCTGAGGAAGCAG CAAATGGAAAGAAATCACATTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCTCATCTTT GTGGTCACTCACTCACTTGACAGCTTTGCATCTGAGTGACAATTCCCTGTCCTGTATTCCTTCAGACATT GCCAAGCTTCACAATCTGGTATATCTGGACCTGTCCCATAATCAAATCCAGAGTTTACCGGCAGAACTCG GAAACATGGTATCACTCAGGGAGCTCCATTTAAATTACAACCAGTTACGAGTTCTACCTTTTGAGCTGGG AAAACTGTTTCAGTTGCAGACTTTAAGCCTGAAAGGAAATCCACTTACCCAGGATATATTGAACCTCTGT CTGGAGCCAGATGGAACAAGAAGGCTACTGAACTACTTGCTCGATAATTTGTCAGGTACTGCAAAAAGAA TTTCAACAGAACAACCACCTCCAAGATCTTGGATCATGTTACAAGAACCAGACAGAACAAGGCCAACAGC CTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGT CCATCATGGGCACTTAATTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATTTTGAGCTGCAATGCTG ATATTATAAGTCTTCAGGAAGTTGAAACAGAGCAGTATTACAGTTTTTTTCTGGTAGAACTGAAAGAACG TGGCTATAATGGATTCTTTAGTCCAAAGTCTCGAGCTCGGACTATGTCTGAACAAGAACGAAAGCATGTT GATGGCTGTGCAATATTCTTCAAGACGGAAAAATTCACTTTGGTTCAGAAACACACAGTTGAATTTAATC AGCTAGCAATGGCGAATTCAGAAGGGTCTGAAGCCATGTTGAACAGAGTCATGACAAAGGATAACATTGG AGTTGCAGTACTGCTAGAACTTCGAAAGGAATTGATTGAAATGTCATCTGGAAAACCACATCTGGGAACA GAAAAACAACTTATTCTCGTGGCTAATGCTCATATGCATTGGGACCCTGAGTACTCAGACGTGAAGTTGG TACAAACTATGATGTTCCTCTCAGAAGTGAAGAATATTATTGATAAAGCCTCTCGGAGCCTCAAATCCAG TGTATTGGGAGAATGTGGAACTATTCCTCTTGTGTTATGTGCAGATCTGAATTCTTTGCCAGACTCTGGT GTTGTAGAGTATTTGAGCACTGGTGGGGTAGAAACAAATCATAAAGACTTCAAGGAACTGAGGTACAATG AAAGTCTCACAAACTTCAGCTGCAATGGGAAGAATGGGATGACCAATGGAAGGATCACTCATGGTTTCAA GTTAAAGAGTGCCTATGAGAATGGCCTGATGCCTTACACCAATTATACATTTGATTTCAAGGGTATAATT GACTACATCTTCTATTCTAAACCTCAGCTGAATACTTTAGCCATCCTGGGACCTCTGGATCACCATTGGC TAGTCGAGAATAACATCAGCGGCTGCCCACATCCACTTATTCCCTCTGACCACTTCTCACTTTTTGCACA ATTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTTAATGGCATTCACCTTCCTGGCAGGAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290741
Insert Size	1674 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290741.1, NP_001277670.1
RefSeq Size	5372 bp
RefSeq ORF	1674 bp
Locus ID	104625
UniProt ID	Q8K3P5
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors. Mediates cell proliferation and cell survival and prevents cellular senescence.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1).

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