Spata7 (NM_001289572) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI661438; B230306G18Rik; HSD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230474 representing NM_001289572 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCAGCCGGAGAGTCAGAGCAACCTCTGTCCTCCCCAGATACAGCCCTCCATGCTTGTTTACAG GACACCTGAGCACCAAGAGCAACGCCGCAGTGGACTGCTCAATTCCAGTAAGTGTGAATACTAGCATCAA ATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGGCACGGTGTGAAAAGGAGTTTAAATTA AGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAAATTCTCTACAGAAGCCCT CAGGGGAACCACAAGACCAGGATGTGTTCATAGAAGAAATGACAAGATACCCATCATTTTCAAAGTCACT CATCCCTTCTTCCGAGGGACTGCATCTAAGTCTGCCTGAATCAAGTAAGATGCTCATGAGTGGCACCCAG AAGCATGCCAGCACCTCTCCATCCAGACACTCAGGCTGTGGGCATGGCTGCGACAGGCGGCCTCGGAGTG CCCACCAGTTCCAGGTAGCCCTCGCCAAGACCCCAAGTGGAGATCTGTTGGAAAAGCACTCTGACCTCTT CTCTAACAAGCAGTCGCCATTCACTCCACGAACTTTAAAAACAGAAGCAAAGTCCTTCCTGTCACAGTAC CGCTACTACACACCTGCCAAAAGAAGAAAGGATTTTTCAGATCAGCGGATGGAAGCTGAAACCCAAACTG AATTAAGCAGCTTTAACTCTGAGCTTGGGACAGCTGAGAAAACGAGCTCAAAGGACTCAGAAGTGAACAT AAACCAGGTACCTAATTACACGAGAAATGGTGCTGAAGACAAAATAGCTCCTTTACCCTCACAAGGACAA AACTTAGCATGGGACAGTATTCAAGATGGGATTCTGCAGCAGTCCTCAGAAAGGGCATCCTGTAAGCTCT CCACAGAGTTTTCTCCAGACAGTAAAATCTACTCTGATGAAGAAGAGCTGCTGTACCTGAGTTTCATGGA AAATGTGACGGATGAAATCTTGAAACTTGGTTTATTTTCTAACAGGTTTCTAGAGCGACTGTTTGAGCGA CATATAAAGAAAAATAAACATCATTTGGAGGAGGGAAAGATGCGCTACCTGCTGCATGGGCTGAAGGTTG ACTTAGGCTGCATATCTGAGGAAGACCCAGCAAAGCAAAAACATTTCAGAATGTTGAATCAACTTCATTT TCAAAAGGCTCTGATTTCAAGAGAAAATGAGTTTGTAAGTGATGAAGAAACAGTGAGTCACCACGAGCGC CAGCAGTACCAGGAGGCCCTGGACATGCTGTCGGCTGTGCCGAAGGACGAAAACAAGATGTTCTCTTTAC CGGGCGAATTTTTAATACCCGCCCATAAAGTCAAGCATTCAGAGGGTGTTATAATTCAACAGGTCAATGA CGAAACAGATAATGAAGCCTCACCCTGGAATGAAAACAATCCAAGTGTTTCTGATAGTGTAATAGACCAG GAAACCTCTGTGGATGTCATTGAAGGTGACAGTGACTTTGAAAGGGCTGAGACTTCCAGGGAACTCTGTT GTCTGAGCACATCACTGTCCCCATCTGGTCCATTCCCCAGCATCAATGGTGGCAGTAATCATGGTAAGGA ATTATCGACTCTACGAATCATGGGAATGAGCATTGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230474 representing NM_001289572 Red=Cloning site Green=Tags(s) MDGSRRVRATSVLPRYSPPCLFTGHLSTKSNAAVDCSIPVSVNTSIKYADQQRREKLRKELARCEKEFKL SKSAMQTNSKMNSKFFVNSLQKPSGEPQDQDVFIEEMTRYPSFSKSLIPSSEGLHLSLPESSKMLMSGTQ KHASTSPSRHSGCGHGCDRRPRSAHQFQVALAKTPSGDLLEKHSDLFSNKQSPFTPRTLKTEAKSFLSQY RYYTPAKRRKDFSDQRMEAETQTELSSFNSELGTAEKTSSKDSEVNINQVPNYTRNGAEDKIAPLPSQGQ NLAWDSIQDGILQQSSERASCKLSTEFSPDSKIYSDEEELLYLSFMENVTDEILKLGLFSNRFLERLFER HIKKNKHHLEEGKMRYLLHGLKVDLGCISEEDPAKQKHFRMLNQLHFQKALISRENEFVSDEETVSHHER QQYQEALDMLSAVPKDENKMFSLPGEFLIPAHKVKHSEGVIIQQVNDETDNEASPWNENNPSVSDSVIDQ ETSVDVIEGDSDFERAETSRELCCLSTSLSPSGPFPSINGGSNHGKELSTLRIMGMSIED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289572
ORF Size	1650 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289572.1, NP_001276501.1
RefSeq Size	1975 bp
RefSeq ORF	1653 bp
Locus ID	104871
MW	62.5 kDa
Gene Summary	Involved in the maintenance of both rod and cone photoreceptor cells (PubMed:25398945, PubMed:29100828, PubMed:29899041). Required for photoreceptor-specific localization of proximal connecting cilium (CC) proteins RPGR, AHI1, NPHP1, NPHP4, and RPGRIP1 at the distal CC, a photoreceptor-specific extension of the primary cilium transition zone (PubMed:25398945, PubMed:29100828, PubMed:29899041). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (PubMed:25398945, PubMed:29899041).[UniProtKB/Swiss-Prot Function]

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