Spata7 (NM_001289572) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI661438; B230306G18Rik; HSD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228263 representing NM_001289572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCAGCCGGAGAGTCAGAGCAACCTCTGTCCTCCCCAGATACAGCCCTCCATGCTTGTTTACAG GACACCTGAGCACCAAGAGCAACGCCGCAGTGGACTGCTCAATTCCAGTAAGTGTGAATACTAGCATCAA ATATGCTGACCAACAACGAAGAGAAAAACTGAGGAAGGAGTTGGCACGGTGTGAAAAGGAGTTTAAATTA AGTAAATCTGCTATGCAGACCAATTCTAAGATGAATTCCAAGTTCTTTGTAAATTCTCTACAGAAGCCCT CAGGGGAACCACAAGACCAGGATGTGTTCATAGAAGAAATGACAAGATACCCATCATTTTCAAAGTCACT CATCCCTTCTTCCGAGGGACTGCATCTAAGTCTGCCTGAATCAAGTAAGATGCTCATGAGTGGCACCCAG AAGCATGCCAGCACCTCTCCATCCAGACACTCAGGCTGTGGGCATGGCTGCGACAGGCGGCCTCGGAGTG CCCACCAGTTCCAGGTAGCCCTCGCCAAGACCCCAAGTGGAGATCTGTTGGAAAAGCACTCTGACCTCTT CTCTAACAAGCAGTCGCCATTCACTCCACGAACTTTAAAAACAGAAGCAAAGTCCTTCCTGTCACAGTAC CGCTACTACACACCTGCCAAAAGAAGAAAGGATTTTTCAGATCAGCGGATGGAAGCTGAAACCCAAACTG AATTAAGCAGCTTTAACTCTGAGCTTGGGACAGCTGAGAAAACGAGCTCAAAGGACTCAGAAGTGAACAT AAACCAGGTACCTAATTACACGAGAAATGGTGCTGAAGACAAAATAGCTCCTTTACCCTCACAAGGACAA AACTTAGCATGGGACAGTATTCAAGATGGGATTCTGCAGCAGTCCTCAGAAAGGGCATCCTGTAAGCTCT CCACAGAGTTTTCTCCAGACAGTAAAATCTACTCTGATGAAGAAGAGCTGCTGTACCTGAGTTTCATGGA AAATGTGACGGATGAAATCTTGAAACTTGGTTTATTTTCTAACAGGTTTCTAGAGCGACTGTTTGAGCGA CATATAAAGAAAAATAAACATCATTTGGAGGAGGGAAAGATGCGCTACCTGCTGCATGGGCTGAAGGTTG ACTTAGGCTGCATATCTGAGGAAGACCCAGCAAAGCAAAAACATTTCAGAATGTTGAATCAACTTCATTT TCAAAAGGCTCTGATTTCAAGAGAAAATGAGTTTGTAAGTGATGAAGAAACAGTGAGTCACCACGAGCGC CAGCAGTACCAGGAGGCCCTGGACATGCTGTCGGCTGTGCCGAAGGACGAAAACAAGATGTTCTCTTTAC CGGGCGAATTTTTAATACCCGCCCATAAAGTCAAGCATTCAGAGGGTGTTATAATTCAACAGGTCAATGA CGAAACAGATAATGAAGCCTCACCCTGGAATGAAAACAATCCAAGTGTTTCTGATAGTGTAATAGACCAG GAAACCTCTGTGGATGTCATTGAAGGTGACAGTGACTTTGAAAGGGCTGAGACTTCCAGGGAACTCTGTT GTCTGAGCACATCACTGTCCCCATCTGGTCCATTCCCCAGCATCAATGGTGGCAGTAATCATGGTAAGGA ATTATCGACTCTACGAATCATGGGAATGAGCATTGAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289572
Insert Size	1653 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289572.1, NP_001276501.1
RefSeq Size	1975 bp
RefSeq ORF	1653 bp
Locus ID	104871
Gene Summary	Involved in the maintenance of both rod and cone photoreceptor cells (PubMed:25398945, PubMed:29100828, PubMed:29899041). Required for photoreceptor-specific localization of proximal connecting cilium (CC) proteins RPGR, AHI1, NPHP1, NPHP4, and RPGRIP1 at the distal CC, a photoreceptor-specific extension of the primary cilium transition zone (PubMed:25398945, PubMed:29100828, PubMed:29899041). Maintenance of protein localization at the photoreceptor-specific distal CC is essential for normal microtubule stability and to prevent photoreceptor degeneration (PubMed:25398945, PubMed:29899041).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1.

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