Ptprr (NM_001161837) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gmcp1; mPTP213; PTP-SL; PTPBR7; RPTPRR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230471 representing NM_001161837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGCAACACTCGGTCAGTTTCCACACCGACACTGCAAATGGATATAACCAAGCTGAACATAACCC TGCTTCGGATCTTCCGCCAAGGAGTAGCTGCAGCCCTGGGACTCTTACCTCAGCAAGTGCACATTAACCG GCTCATTGAAAAGAAGAACCAGGTTGAGTTGTTTGTGTCTCCCGGAAACCGGAAACCAGGAGAAACGCAG GCCCTGCAGGCTGAGGAAGTGCTGCGTTCCCTCAATGTGGATGGTTTGCATCAGAGTTTACCACAGTTTG GAATTACAGACGTCGCCCCTGAGAAAAATGTTTTACAAGGGCAGCACGAAGCAGACAAGATCTGGAGCAA AGAAGGATTTTACGCTGTCGTCATCTTCCTCAGCATCTTTATCATCATAGTAACCTGTTTGATGATTATT TACAGGTTAAAAGAAAGGCTTCAGCTTTCCTTAAGACAAGATAAAGAGAAAAACCAGGAGATCCACCTAT CACCCATTGCACGGCAGCAAGCACAATCGGAGGCCAAGACGACCCACAGCATGGTCCAGCCCGATCAGGC GCCAAAGGTGCTGAACGTGGTTGTGGACCCTCAAGGCCAATGCACTCCTGAGATTCGAAACAGCACCTCC ACCTCTGTCTGCCCTTCTCCCTTCAGAATGAAGCCCATAGGACTCCAGGAGCGACGAGGTTCCAATGTAT CTCTTACGCTGGACATGAGTAGCCTGGGCAGTGTGGAACCCTTTGTGGCCGTCTCAACCCCCCGGGAGAA GGTAGCCATGGAATACCTGCAGTCAGCCAGCCGAGTTCTCACACGGTCACAGCTGAGGGACGTCGTGGCA AGTTCCCACCTACTTCAAAGTGAATTCATGGAAATACCAATGAATTTTGTGGATCCCAAAGAAATTGATA TTCCACGTCACGGAACTAAAAATCGTTATAAGACCATTTTGCCAAATCCCCTCAGCAGAGTGTGCTTAAG ACCAAAAAATATAACCGATTCCTTGAGTACTTACATAAATGCTAACTATATTCGGGGCTACAGTGGTAAG GAGAAAGCCTTCATTGCCACCCAGGGCCCCATGATCAACACTGTGAATGACTTCTGGCAGATGGTGTGGC AAGAAGACAGTCCCGTGATTGTGATGATCACGAAACTCAAAGAGAAAAATGAGAAATGTGTGCTCTACTG GCCAGAAAAGAGAGGGATTTACGGCAAGGTTGAGGTTCTGGTCACCGGTGTGACCGAATGTGATAACTAC ACCATCCGCAACCTCGTCTTAAAGCAAGGAAGTCACACCCAACATGTGAAGCACTACTGGTACACTTCAT GGCCGGATCATAAGACTCCAGACAGTGCCCAGCCCCTTCTGCAGCTCATGTTGGATGTGGAAGAAGACAG ACTGGCCTCTGAAGGCCGAGGGCCTGTGGTTGTCCACTGCAGTGCGGGGATTGGGAGAACTGGGTGTTTC ATCGCTACATCCATTGGCTGTCAACAATTGAAAGAAGAAGGAGTTGTAGACGCACTAAGTATTGTCTGCC AGCTTCGTGTAGACAGGGGTGGTATGGTCCAAACCAGCGAGCAGTATGAATTTGTGCACCATGCTCTGTG CCTGTTCGAGAGCAGACTTTCACCAGAAACTGTCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230471 representing NM_001161837 Red=Cloning site Green=Tags(s) MHRNTRSVSTPTLQMDITKLNITLLRIFRQGVAAALGLLPQQVHINRLIEKKNQVELFVSPGNRKPGETQ ALQAEEVLRSLNVDGLHQSLPQFGITDVAPEKNVLQGQHEADKIWSKEGFYAVVIFLSIFIIIVTCLMII YRLKERLQLSLRQDKEKNQEIHLSPIARQQAQSEAKTTHSMVQPDQAPKVLNVVVDPQGQCTPEIRNSTS TSVCPSPFRMKPIGLQERRGSNVSLTLDMSSLGSVEPFVAVSTPREKVAMEYLQSASRVLTRSQLRDVVA SSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNITDSLSTYINANYIRGYSGK EKAFIATQGPMINTVNDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVTGVTECDNY TIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASEGRGPVVVHCSAGIGRTGCF IATSIGCQQLKEEGVVDALSIVCQLRVDRGGMVQTSEQYEFVHHALCLFESRLSPETVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001161837
ORF Size	1647 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001161837.1, NP_001155309.1
RefSeq Size	2860 bp
RefSeq ORF	1650 bp
Locus ID	19279
UniProt ID	Q62132
MW	62.4 kDa
Gene Summary	Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.[UniProtKB/Swiss-Prot Function]

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