Ptprr (NM_001161837) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gmcp1; mPTP213; PTP-SL; PTPBR7; RPTPRR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228260 representing NM_001161837 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGCAACACTCGGTCAGTTTCCACACCGACACTGCAAATGGATATAACCAAGCTGAACATAACCC TGCTTCGGATCTTCCGCCAAGGAGTAGCTGCAGCCCTGGGACTCTTACCTCAGCAAGTGCACATTAACCG GCTCATTGAAAAGAAGAACCAGGTTGAGTTGTTTGTGTCTCCCGGAAACCGGAAACCAGGAGAAACGCAG GCCCTGCAGGCTGAGGAAGTGCTGCGTTCCCTCAATGTGGATGGTTTGCATCAGAGTTTACCACAGTTTG GAATTACAGACGTCGCCCCTGAGAAAAATGTTTTACAAGGGCAGCACGAAGCAGACAAGATCTGGAGCAA AGAAGGATTTTACGCTGTCGTCATCTTCCTCAGCATCTTTATCATCATAGTAACCTGTTTGATGATTATT TACAGGTTAAAAGAAAGGCTTCAGCTTTCCTTAAGACAAGATAAAGAGAAAAACCAGGAGATCCACCTAT CACCCATTGCACGGCAGCAAGCACAATCGGAGGCCAAGACGACCCACAGCATGGTCCAGCCCGATCAGGC GCCAAAGGTGCTGAACGTGGTTGTGGACCCTCAAGGCCAATGCACTCCTGAGATTCGAAACAGCACCTCC ACCTCTGTCTGCCCTTCTCCCTTCAGAATGAAGCCCATAGGACTCCAGGAGCGACGAGGTTCCAATGTAT CTCTTACGCTGGACATGAGTAGCCTGGGCAGTGTGGAACCCTTTGTGGCCGTCTCAACCCCCCGGGAGAA GGTAGCCATGGAATACCTGCAGTCAGCCAGCCGAGTTCTCACACGGTCACAGCTGAGGGACGTCGTGGCA AGTTCCCACCTACTTCAAAGTGAATTCATGGAAATACCAATGAATTTTGTGGATCCCAAAGAAATTGATA TTCCACGTCACGGAACTAAAAATCGTTATAAGACCATTTTGCCAAATCCCCTCAGCAGAGTGTGCTTAAG ACCAAAAAATATAACCGATTCCTTGAGTACTTACATAAATGCTAACTATATTCGGGGCTACAGTGGTAAG GAGAAAGCCTTCATTGCCACCCAGGGCCCCATGATCAACACTGTGAATGACTTCTGGCAGATGGTGTGGC AAGAAGACAGTCCCGTGATTGTGATGATCACGAAACTCAAAGAGAAAAATGAGAAATGTGTGCTCTACTG GCCAGAAAAGAGAGGGATTTACGGCAAGGTTGAGGTTCTGGTCACCGGTGTGACCGAATGTGATAACTAC ACCATCCGCAACCTCGTCTTAAAGCAAGGAAGTCACACCCAACATGTGAAGCACTACTGGTACACTTCAT GGCCGGATCATAAGACTCCAGACAGTGCCCAGCCCCTTCTGCAGCTCATGTTGGATGTGGAAGAAGACAG ACTGGCCTCTGAAGGCCGAGGGCCTGTGGTTGTCCACTGCAGTGCGGGGATTGGGAGAACTGGGTGTTTC ATCGCTACATCCATTGGCTGTCAACAATTGAAAGAAGAAGGAGTTGTAGACGCACTAAGTATTGTCTGCC AGCTTCGTGTAGACAGGGGTGGTATGGTCCAAACCAGCGAGCAGTATGAATTTGTGCACCATGCTCTGTG CCTGTTCGAGAGCAGACTTTCACCAGAAACTGTCGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001161837
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001161837.1, NP_001155309.1
RefSeq Size	2860 bp
RefSeq ORF	1650 bp
Locus ID	19279
UniProt ID	Q62132
Gene Summary	Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.

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