Btn2a2 (NM_001289615) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Btn2; D030063K05
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230332 representing NM_001289615 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTACAACTTCCCTGCGTTCTTGCCCGATAGCCTCCCTTCTCTTCTTCTTGGTCCTCAGCCTGT TTGTGCTGGTCTCAGCCCAGTTTACTGTCATAGGACCAGCTGAGCCCATCCTGGCCATGGTAGGAGAGAA TACCACACTACACTGCCACCTGTCACCAGAGAGAAATGCCGAAGAGATGGAGGTGCGGTGGTTCCGGTGG CGTTTCTTCCCTGCAGTGCTGGTGTACAGAGGCCATCAAGAGAGACCAGAGGAGCAGATGGTGGCATACC GAGGAAGAACCACCTTCATGCGCACAGACATCAGCAAGGGAAGAGTTGCGCTCATTATCCACAATGTCAC AGCCTATGACAATGGCATCTACTGCTGTTACTTCCAGGAAGGCAGGTCCTATGACCAGGCAACCATGAAG CTTATGGTGGCAAGCCTTGGCTCTGAGCCACTTATTAAAATGAAGACACTTGAGGATGGGAGCATCTTGC TAGAGTGCACATCTGAAGGGTGGTACCCAGAGCCCCGAGCTGTGTGGAGAGACCCCTATGATGAAGTTGT ACCTGCCCTGGAGGAGGAGTATACAGCTGACAGAGAAGGCCTCTTCACAGTCACCATGACTATAATCATC AGGGACTGCTCTGTGAGGAACATGACCTGCTCTGTCAATAACACTCTGCTCAGCCAGGAGGTGGAAAGTG TGATTCTCATTCCAGAATCCTTCGTGCCCAGCCTTCCTCTGTGGATGGTGGCTGTGGCTGTCACTCTGCC TGTAGTAATGCTGATTCTCCTCACATCTGGAAGCATCTGCCTTGTCAAGAAACACCGCAGGAAGAAATCT ATTCTGTCAGCTGAAAAAGAAGCCGAATATGAAGAGAAGGAAGCTGCACGGCAACTTCAAGAGGAACTGC GATGGAGACGAACCCTCTTACATGCTGCTGACGTGGTCCTGGACCCAGATACAGCTCATCCTGAGCTCTT CCTGTCAGATGACCAGAGAAGTGTAATACGAGGCTCTTCGAGGCAGAGTGTGCCTGACAACCCTGAGAGA TTTGACTGCCGTCCATGTGTCCTGGGCAGGGAAAGCTTCTCCTCAGGGAAGCATTACTGGGAGGTGGAGG TGGAAAATGTAATGGTGTGGGCCATTGGTGTTTGTAGAGACAGCGTGGAAAGGAAAGGGGAGGCCCTGTT GGTTCCTCAGAATGGCTTCTGGACCCTGGAGATGTTTGGAAGCCAGTATCGAGCCCTGTCCTCCCCAGAA AAGATCATACCTCTGAAAGAGCGTCTTCACCGTATAGCTGTCTTCCTGGACTGTGAGGGTGGAGATATTT CTTTCTACAACATGAGAGACAGATCACACATTTACACATGTCCTCCTGTGACTTTCACTGGGCCCCTGAG ACCCTTCTTTAGGCTTGGTTCTGATGACAGTCCCCTGTTCATCTGTCCAGCATTCACAGGGGCACAGGGA GTTACAATACCTGAGGGTGGCTTATTCCTATATAAGACAAGACCAATTTCTCAGAGCCTTGTAAGGAAGC CA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230332 representing NM_001289615 Red=Cloning site Green=Tags(s) MEPTTSLRSCPIASLLFFLVLSLFVLVSAQFTVIGPAEPILAMVGENTTLHCHLSPERNAEEMEVRWFRW RFFPAVLVYRGHQERPEEQMVAYRGRTTFMRTDISKGRVALIIHNVTAYDNGIYCCYFQEGRSYDQATMK LMVASLGSEPLIKMKTLEDGSILLECTSEGWYPEPRAVWRDPYDEVVPALEEEYTADREGLFTVTMTIII RDCSVRNMTCSVNNTLLSQEVESVILIPESFVPSLPLWMVAVAVTLPVVMLILLTSGSICLVKKHRRKKS ILSAEKEAEYEEKEAARQLQEELRWRRTLLHAADVVLDPDTAHPELFLSDDQRSVIRGSSRQSVPDNPER FDCRPCVLGRESFSSGKHYWEVEVENVMVWAIGVCRDSVERKGEALLVPQNGFWTLEMFGSQYRALSSPE KIIPLKERLHRIAVFLDCEGGDISFYNMRDRSHIYTCPPVTFTGPLRPFFRLGSDDSPLFICPAFTGAQG VTIPEGGLFLYKTRPISQSLVRKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289615
ORF Size	1542 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289615.1, NP_001276544.1
RefSeq Size	2924 bp
RefSeq ORF	1545 bp
Locus ID	238555
UniProt ID	A4QPC6
MW	58.9 kDa
Gene Summary	Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.[UniProtKB/Swiss-Prot Function]

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