Syt1 (NM_001252342) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW124717; G630098F17Rik; SytI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229803 representing NM_001252342 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGTGCCAGTCGTCCTGAGGCCCTGGCTGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACA ACGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTAT GAATGAACTGCATAAAATCCCATTGCCACCGTGGGCCTTAATTGCCATAGCCATAGTTGCGGTCCTTCTA GTCGTGACCTGCTGCTTCTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAAAGG AAAAGGGAGGGAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGA TGACGATGCTGAAACTGGACTGACTGATGGAGAAGAAAAGGAGGAGCCCAAGGAAGAGGAGAAACTGGGA AAGCTTCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGCTGGTGGGAATCATCCAGGCTGCTG AACTGCCCGCCCTGGACATGGGAGGCACATCTGATCCATACGTCAAAGTCTTCCTGCTGCCCGACAAAAA GAAGAAGTTTGAGACAAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAGTTTACTTTCAAG GTGCCATACTCGGAATTAGGTGGCAAGACACTGGTGATGGCTGTGTATGATTTTGACCGCTTCTCCAAGC ACGACATCATTGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCACGTCACCGAGGAGTGGCG CGATCTCCAGAGTGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTCCGCTACGTC CCTACTGCCGGCAAGCTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATGTGGGTGGCT TATCTGATCCCTATGTAAAGATTCACCTGATGCAGAACGGCAAGAGACTGAAGAAGAAAAAGACAACGAT TAAGAAGAACACACTTAACCCCTACTACAATGAGTCCTTCAGCTTTGAAGTTCCGTTCGAGCAAATCCAG AAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCCATCGGCAAAGTCT TTGTGGGCTACAACAGCACCGGCGCAGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCCGGCGACC CATCGCCCAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229803 representing NM_001252342 Red=Cloning site Green=Tags(s) MVSASRPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLL VVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQDDDAETGLTDGEEKEEPKEEEKLG KLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFK VPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYV PTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQ KVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252342
ORF Size	1254 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252342.1, NP_001239271.1
RefSeq Size	4747 bp
RefSeq ORF	1257 bp
Locus ID	20979
UniProt ID	P46096
MW	47.6 kDa
Gene Summary	Calcium sensor that participates in triggering neurotransmitter release at the synapse (PubMed:11242035). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]

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