Syt1 (NM_001252342) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW124717; G630098F17Rik; SytI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227592 representing NM_001252342 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGTGCCAGTCGTCCTGAGGCCCTGGCTGCCCCTGTCACCACTGTTGCGACCCTTGTCCCACACA ACGCCACTGAGCCAGCCAGTCCTGGGGAAGGGAAGGAAGATGCCTTTTCCAAGCTGAAGCAGAAGTTTAT GAATGAACTGCATAAAATCCCATTGCCACCGTGGGCCTTAATTGCCATAGCCATAGTTGCGGTCCTTCTA GTCGTGACCTGCTGCTTCTGTGTCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAAAGG AAAAGGGAGGGAAGAACGCCATTAACATGAAAGACGTGAAAGACTTAGGGAAGACCATGAAGGATCAGGA TGACGATGCTGAAACTGGACTGACTGATGGAGAAGAAAAGGAGGAGCCCAAGGAAGAGGAGAAACTGGGA AAGCTTCAATATTCACTGGACTATGACTTCCAGAATAACCAGCTGCTGGTGGGAATCATCCAGGCTGCTG AACTGCCCGCCCTGGACATGGGAGGCACATCTGATCCATACGTCAAAGTCTTCCTGCTGCCCGACAAAAA GAAGAAGTTTGAGACAAAAGTCCACCGGAAAACCCTCAATCCAGTCTTCAATGAACAGTTTACTTTCAAG GTGCCATACTCGGAATTAGGTGGCAAGACACTGGTGATGGCTGTGTATGATTTTGACCGCTTCTCCAAGC ACGACATCATTGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCACGTCACCGAGGAGTGGCG CGATCTCCAGAGTGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTCCGCTACGTC CCTACTGCCGGCAAGCTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATGTGGGTGGCT TATCTGATCCCTATGTAAAGATTCACCTGATGCAGAACGGCAAGAGACTGAAGAAGAAAAAGACAACGAT TAAGAAGAACACACTTAACCCCTACTACAATGAGTCCTTCAGCTTTGAAGTTCCGTTCGAGCAAATCCAG AAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCCATCGGCAAAGTCT TTGTGGGCTACAACAGCACCGGCGCAGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCCGGCGACC CATCGCCCAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTGATGCCATGCTGGCTGTCAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252342
Insert Size	1257 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252342.1, NP_001239271.1
RefSeq Size	4747 bp
RefSeq ORF	1257 bp
Locus ID	20979
UniProt ID	P46096
Gene Summary	Calcium sensor that participates in triggering neurotransmitter release at the synapse (PubMed:11242035). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR and uses a difference splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

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