Preb (NM_001294302) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C85705
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229425 representing NM_001294302 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGGCGCCGGGGTGTGGAGCTGTACCGGGCCCCGTTCCCGTTGTACGCGCTTCGGATAGACCCCA AGACTGGGCTGCTCATCGCTGCGGGCGGAGGAGGAGCTGCCAAGACCGGCATAAAGAATGGCGTGCATTT TCTGCAGCTAGAGCTGATCAACGGGTGCCTGAGCGCTTCCTTGCTGCACTCTCATGACACGGAGACACGG GCCACCATGAATTTGGCGCTTGCTGGTGACATTCTTGCTGCCGGACAGGATGCCCAGTGTCAGCTTCTTC GTTTCCAGGTCCATCAACAGAAGGGCAGTAAAGCGGAGAAGTCAGGTTCCAAGGAGCAGGGACCTCGACA GAGAAAGGGGGCTCCTCCAGCAGAGAAGAAATCGGGAGCACAAGTTCACCCCGAAGGGGTTGAACTCAAA GTAAAGAATTTGGAGGCAGTACAGACAGACTTCAGCAATGAACCGCTGCAGAAAGTTGTGTGCTTCAACC ATGATAACACCCTGCTTGCCACTGGAGGAACTGATGGTCATGTTCGTGTCTGGAAGGTACCTAGCCTGGA GAAAGTTCTGGAGTTCAAAGCCCATGAAGGGGAGATTGGAGATTTGACTTTGGGTCCTGATGGCAAGCTG GTTACAGTGGGCTGGGACTTTAAGGCCTCTGTGTGGCAGAAGGATCAACTGGTGACACAGCTACAGTGGC AAGAGAATGGACCAGCCTCTTCTAATACACCATACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGA TCAGCTCGGTGGGCTGCGACTCTTCACAGTGCAGATACCCCACAAGCGCCTACGTCAGCCCCCACCCTGC TACCTCACAGCCTGGGACAGTTCCACCTTCTTGCCTCTTCGGACCAGATCCTGTGGCCATGAGGTCATTT CCTGCCTCAGTGTCAGTGATTCGGGTACCTTCCTAGGCCTAGGCACGGTCACTGGCTCTGTCGCCATCTA CATAGCTTTCTCTCTCCAGGGAGTGTTCCTGTATGGCTTCTGCTCCTGCTGTGTGTCGGCCTTATTATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229425 representing NM_001294302 Red=Cloning site Green=Tags(s) MGRRRGVELYRAPFPLYALRIDPKTGLLIAAGGGGAAKTGIKNGVHFLQLELINGCLSASLLHSHDTETR ATMNLALAGDILAAGQDAQCQLLRFQVHQQKGSKAEKSGSKEQGPRQRKGAPPAEKKSGAQVHPEGVELK VKNLEAVQTDFSNEPLQKVVCFNHDNTLLATGGTDGHVRVWKVPSLEKVLEFKAHEGEIGDLTLGPDGKL VTVGWDFKASVWQKDQLVTQLQWQENGPASSNTPYRYQACRFGQVPDQLGGLRLFTVQIPHKRLRQPPPC YLTAWDSSTFLPLRTRSCGHEVISCLSVSDSGTFLGLGTVTGSVAIYIAFSLQGVFLYGFCSCCVSALLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001294302
ORF Size	1050 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001294302.1, NP_001281231.1
RefSeq Size	5311 bp
RefSeq ORF	1053 bp
Locus ID	50907
MW	38.4 kDa
Gene Summary	Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.[UniProtKB/Swiss-Prot Function]

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