Vgll2 (NM_001300957) Mouse Tagged ORF Clone
CAT#: MR229233
- TrueORF®
Vgll2 (myc-DDK-tagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), transcript variant 2
"NM_001300957" in other vectors (1)
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CNY 3,230.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C130057C21Rik; Vgl; vgl-2; Vi; VIT; VITO-1; Vito1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229233 representing NM_001300957
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGTCTGGATGTTATGTACCAGGTCTACGGTCCCCCGCAGCCTTATTTCGCAGCCGCCTACACTC CCTACCACCAGAAACTAGCCTACTACTCAAAAATGCAGGAAGCTCAAGAGTGCGCCAGCCCTGGCAGCAG TGCCAGCGGGAGCTCCTCATTTTCCAACCCAACCCCAGCCAGTGTCAAGGAAGAGGAGGGCAGCCCAGAG AAAGAGCGCCCGCCCGAAGCTGAGTACATCAACTCCAGATGTGTCCTCTTCACCTACTTCCAGGGGGACA TCAGCTCTGTGGTGGACGAACATTTCAGTAGGGCCCTTAGCCACCCAAGCAGCTACACCCCAAGCTGTAC CAGCAGCAAAGCACACAGAAGCTCTGGACCCTGGAGAGAAGGCACCTTCCCGATGAGCCAGCGCAGCTTC CCCGCCTCCTTCTGGAACAGCGCGTACCAGGCGCCTGTGCCCGCGCCACTAGGCAGTCCTCTGGCCGCCG CACACTCGGAGCTGCCCTTTGCCACCGACCCCTACTCTCCCGCCACTCTGCACGGCCACCTGCACCAGGG CGCGGCCGACTGGCACCACGCGCACCCGCACCACGCGCACCCGCACCATCCCTATGCGCTGGGCGGCGCC CTGGGAGCACAAGCCTCTGCCTACCCGCGGCCAGCAGTGCACGAGGTCTACGCGCCCCACTTCGACCCGC GCTATGGGCCGCTGCTCATGCCCGCGGCCACTGGCCGCCCCGGCCGCCTGGCCCCTGCCTCGGCGCCGGC TCCCGGCAGCCCTCCCTGCGAGCTTGCGGCCAAGGGCGAGCCGGCGGGCAGCGCATGGGCTGCGCCCGGG GGACCCTTCGTGAGCCCCACGGGGGATGTGGCCCAGAGCCTGGGTCTCAGCGTGGACTCAGGTAAGCGGA GGAGGGAATGCAGTCTCCCCTCTGCCCCTCCGGCACTGTACCCGACTCTGGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229233 representing NM_001300957
Red=Cloning site Green=Tags(s) MSCLDVMYQVYGPPQPYFAAAYTPYHQKLAYYSKMQEAQECASPGSSASGSSSFSNPTPASVKEEEGSPE KERPPEAEYINSRCVLFTYFQGDISSVVDEHFSRALSHPSSYTPSCTSSKAHRSSGPWREGTFPMSQRSF PASFWNSAYQAPVPAPLGSPLAAAHSELPFATDPYSPATLHGHLHQGAADWHHAHPHHAHPHHPYALGGA LGAQASAYPRPAVHEVYAPHFDPRYGPLLMPAATGRPGRLAPASAPAPGSPPCELAAKGEPAGSAWAAPG GPFVSPTGDVAQSLGLSVDSGKRRRECSLPSAPPALYPTLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001300957 |
ORF Size | 963 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300957.1, NP_001287886.1 |
RefSeq Size | 1664 bp |
RefSeq ORF | 966 bp |
Locus ID | 215031 |
UniProt ID | Q8BGW8 |
MW | 34.4 kDa |
Gene Summary | This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227022 | Vgll2 (untagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), transcript variant 2 |
CNY 3,140.00 |