Stk11 (NM_001301854) Mouse Tagged ORF Clone
CAT#: MR229169
- TrueORF®
Stk11 (myc-DDK-tagged) - Mouse serine/threonine kinase 11 (Stk11), transcript variant 3
"NM_001301854" in other vectors (1)
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CNY 3,140.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA408040; Lkb1; Par-4; R75140 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR229169 representing NM_001301854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATATGGTGATGGAGTACTGCGTATGTGGCATGCAGGAGATGCTGGACAGTGTGCCGGAGAAGCGCT TCCCTGTGTGCCAAGCTCATGGGTACTTCCGCCAGCTGATTGACGGCCTGGAATACCTACACAGCCAGGG CATTGTTCACAAGGACATCAAGCCGGGCAACCTGCTACTCACCACCAATGGCACACTCAAGATCTCCGAC CTCGGTGTTGCCGAGGCCCTGCACCCTTTCGCTGTGGATGACACCTGCCGGACAAGCCAGGGCTCCCCGG CCTTCCAGCCTCCTGAGATTGCCAATGGACTGGACACCTTTTCAGGTTTCAAGGTGGACATCTGGTCAGC TGGGGTCACACTTTACAACATCACCACGGGCCTGTACCCATTTGAGGGGGACAATATCTACAAGCTCTTT GAGAACATTGGGAGAGGAGACTTCACCATCCCTTGTGACTGCGGCCCACCACTCTCTGACCTACTCCGAG GGATGTTGGAGTATGAGCCGGCCAAGAGGTTCTCCATCCGACAGATTAGGCAGCACAGCTGGTTCCGGAA GAAACACCCTCTGGCTGAGGCGCTCGTACCTATCCCACCAAGCCCAGACACTAAGGACCGCTGGCGCAGT ATGACTGTAGTGCCCTACCTGGAGGACCTGCATGGCCGTGCGGAGGAGGAGGAGGAGGAAGACTTGTTTG ACATTGAGGACGGCATTATCTACACCCAGGACTTCACAGTGCCTGGACAGGTCCTGGAAGAGGAAGTGGG TCAGAATGGACAGAGCCACAGTTTGCCCAAGGCTGTTTGTGTGAATGGCACAGAGCCCCAGCTCAGCAGC AAGGTGAAGCCAGAAGGCCGACCTGGCACCGCCAACCCTGCGCGCAAGGTGTGCTCCAGCAACAAGATCC GCCGGCTCTCGGCCTGCAAGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR229169 representing NM_001301854
Red=Cloning site Green=Tags(s) MYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFRQLIDGLEYLHSQGIVHKDIKPGNLLLTTNGTLKISD LGVAEALHPFAVDDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLF ENIGRGDFTIPCDCGPPLSDLLRGMLEYEPAKRFSIRQIRQHSWFRKKHPLAEALVPIPPSPDTKDRWRS MTVVPYLEDLHGRAEEEEEEDLFDIEDGIIYTQDFTVPGQVLEEEVGQNGQSHSLPKAVCVNGTEPQLSS KVKPEGRPGTANPARKVCSSNKIRRLSACKQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301854 |
ORF Size | 936 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301854.2 |
RefSeq Size | 2378 bp |
RefSeq ORF | 939 bp |
Locus ID | 20869 |
UniProt ID | Q9WTK7 |
MW | 35.4 kDa |
Gene Summary | This gene encodes a member of the serine/threonine kinase family. The encoded protein, a known tumor suppressor, activates (via phosphorylation) adenine monophosphate-activated protein kinase (AMPK) and AMPK-related kinase proteins. This upstream regulation of the AMPK pathway is thought to regulate a number of different processes, including cell metabolism, cell polarity, apoptosis and DNA damage response. Mutations in a similar gene in human have been associated with Peutz-Jeghers syndrome. Alternative splicing results in multiple transcript variants, including the S isoform which plays a potential role in spermiogenesis. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226958 | Stk11 (untagged) - Mouse serine/threonine kinase 11 (Stk11), transcript variant 3 |
CNY 3,040.00 |