Aup1 (NM_001301649) Mouse Tagged ORF Clone
CAT#: MR228971
- TrueORF®
Aup1 (myc-DDK-tagged) - Mouse ancient ubiquitous protein 1 (Aup1), transcript variant 2
"NM_001301649" in other vectors (1)
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CNY 2,850.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA589454 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228971 representing NM_001301649
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGGATAGGCGGGTGGAGTTGGTGGAGTCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGC CCACACCTTTGCTGCTCTTCCCCGAGGAAGAGGCCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTC GTGGCCATTTTCTATTCAGGACGTGGTACAACCTCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTG ACGGTGTCAGATGCCTCCTGGGTCTCAGAACTGCTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAG TAAGGTGGCTTCATCCCATTCGTCGACAGCTAGGGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACA GCTGGTGGCCAAAGAATTGGGCCAGATAGGGACACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAG CGACAAAGACACCCCAGATTACGCCCCCAGTCAGTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTT CTGATGTGCAGCTGACCACTCTCGCTCACAGAGTCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCAT CCAGAGAGACCTGGCCAGGACTGGGTGTGTAGACTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCT TTCATGCCTGAAGATGTCACTGAGGGATCTCAGTCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCT CGGGCCTGGCGACCCCTCAGCCCACAGCCCTAACGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCT GCAGGAGCGCAAGCAGGCACTGTATGAATATGCAAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCT GAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228971 representing NM_001301649
Red=Cloning site Green=Tags(s) MEMDRRVELVESLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSV TVSDASWVSELLWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKELGQIGTRLTPADKAEHMK RQRHPRLRPQSVQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLARTGCVDLTITNLLEGAVA FMPEDVTEGSQSPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQALYEYARRRFRERQAQEA E TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301649 |
ORF Size | 843 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301649.1, NP_001288578.1 |
RefSeq Size | 1508 bp |
RefSeq ORF | 846 bp |
Locus ID | 11993 |
MW | 32.3 kDa |
Gene Summary | The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226760 | Aup1 (untagged) - Mouse ancient ubiquitous protein 1 (Aup1), transcript variant 2 |
CNY 2,760.00 |