Hmgb3 (NM_001293625) Mouse Tagged ORF Clone
CAT#: MR228466
- TrueORF®
Hmgb3 (myc-DDK-tagged) - Mouse high mobility group box 3 (Hmgb3), transcript variant 4
"NM_001293625" in other vectors (1)
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CNY 2,400.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Hmg2a; Hmg4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228466 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAAAGGTGACCCCAAGAAACCAAAGGGCAAGATGTCTGCTTATGCCTTCTTTGTGCAGACATGCA GGGAAGAACATAAGAAGAAAAACCCAGAGGTTCCCGTCAATTTTGCTGAGTTCTCCAAGAAGTGCTCGGA GAGGTGGAAGACCATGTCTAGCAAAGAGAAATCAAAGTTTGATGAAATGGCAAAGGCAGATAAAGTCCGA TATGATCGGGAGATGAAAGATTATGGACCAGCTAAAGGAGGCAAGAAGAAGAAGGACCCAAATGCCCCCA AAAGACCTCCGTCTGGATTTTTCTTATTCTGCTCTGAATTCCGCCCCAAGATCAAATCCACAAACCCTGG CATCTCCATTGGAGATGTGGCAAAAAAGCTGGGTGAGATGTGGAATAACTTAAGTGACAATGAAAAGCAG CCTTATGTCACCAAGGCAGCAAAGCTGAAGGAGAAGTATGAGAAGGATGTTGCTGACTATAAGTCTAAAG GGAAGTTTGATGGTGCCAAGGGTCCTGCTAAAGTTGCCCGGAAAAAGGTGGAAGAAGAGGAAGAGGAGGA GGAAGAGGAAGAAGAGGAGGAGGAAGAGGAGGAAGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228466 protein sequence
Red=Cloning site Green=Tags(s) MAKGDPKKPKGKMSAYAFFVQTCREEHKKKNPEVPVNFAEFSKKCSERWKTMSSKEKSKFDEMAKADKVR YDREMKDYGPAKGGKKKKDPNAPKRPPSGFFLFCSEFRPKIKSTNPGISIGDVAKKLGEMWNNLSDNEKQ PYVTKAAKLKEKYEKDVADYKSKGKFDGAKGPAKVARKKVEEEEEEEEEEEEEEEEEEDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001293625 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001293625.1, NP_001280554.1 |
RefSeq Size | 1565 bp |
RefSeq ORF | 603 bp |
Locus ID | 15354 |
UniProt ID | O54879 |
MW | 23 kDa |
Gene Summary | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (PubMed:19890330). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (PubMed:12714519, PubMed:15358624, PubMed:16945912).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226255 | Hmgb3 (untagged) - Mouse high mobility group box 3 (Hmgb3), transcript variant 4 |
CNY 2,000.00 |