Smad6 (NM_008542) Mouse Tagged ORF Clone
CAT#: MR227576
- TrueORF®
Smad6 (Myc-DDK-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6)
ORF Plasmid: tGFP
"NM_008542" in other vectors (7)
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CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | b2b390Clo; Madh6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227576 representing NM_008542
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCAGATCTCAAGCTT AAGCTTATGTTCAGGTCTAAACGTTCGGGGCTGGTGCGGCGACTTTGGCGAAGTCGTGTGGTCCCTGATC GGGAGGAAGGCAGCGGCGGCGGCGGTGGTGTCGACGAGGATGGGAGCCTGGGCAGCCGAGCTGAGCCTGC CCCGCGGGCACGAGAGGGCGGAGGCTGCAGCCGCTCCGAAGTCCGCTCGGTAGCCCCGCGGCGGCCCCGG GACGCGGTGGGACCGCGAGGCGCCGCGATCGCGGGCAGGCGCCGGCGCACAGGGGGCCTCCCGAGGCCCG TGTCGGAGTCGGGGGCCGGGGCTGGGGGCTCCCCGCTGGATGTGGCGGAGCCTGGAGGCCCAGGCTGGCT GCCTGAGAGTGACTGCGAGACGGTGACCTGCTGTCTCTTCTCCGAACGGGACGCAGCAGGCGCGCCCCGG GACTCTGGCGATCCCCAAGCCAGACAGTCCCCGGAGCCGGAGGAGGGCGGCGGGCCTCGGAGTCGCGAAG CCCGCTCGCGACTGCTGCTTCTGGAGCAGGAGCTCAAGACGGTCACGTACTCGCTGCTCAAGAGGCTCAA GGAGCGTTCGCTGGACACGCTGTTGGAGGCTGTGGAGTCCCGAGGCGGCGTACCGGGCGGCTGCGTGCTG GTGCCGCGCGCCGACCTCCGCTTGGGCGGCCAGCCCGCGCCACCGCAGCTGCTGCTCGGCCGCCTCTTCC GCTGGCCAGACCTGCAGCACGCAGTGGAGCTGAAACCCCTGTGCGGCTGCCACAGCTTTACCGCCGCCGC CGACGGGCCCACGGTGTGTTGCAACCCCTACCACTTCAGCCGGCTCTGCGGGCCAGAATCACCGCCGCCC CCCTATTCTCGGCTGTCTCCTCCTGACCAGTACAAGCCACTGGATCTGTCCGATTCTACATTGTCTTACA CTGAAACCGAGGCCACCAACTCCCTCATCACTGCTCCGGGTGAATTCTCAGATGCCAGCATGTCTCCGGA TGCCACCAAGCCGAGCCACTGGTGCAGCGTGGCGTACTGGGAGCACCGGACACGCGTGGGCCGCCTCTAT GCGGTGTACGACCAGGCTGTCAGCATTTTCTACGACCTACCTCAGGGCAGCGGCTTCTGCCTGGGCCAGC TCAACCTGGAGCAGCGCAGTGAGTCGGTGCGGCGCACGCGCAGCAAGATCGGTTTTGGCATACTGCTCAG CAAGGAGCCAGACGGCGTGTGGGCCTACAACCGGGGCGAGCACCCCATCTTCGTCAACTCCCCGACGCTG GATGCGCCCGGAGGCCGCGCCCTGGTCGTGCGCAAGGTGCCACCGGGTTACTCCATCAAGGTGTTCGACT TTGAGCGCTCAGGGCTGCTGCAGCACGCAGACGCCGCTCACGGCCCCTACGACCCGCACAGTGTGCGCAT CAGCTTCGCCAAGGGCTGGGGACCCTGCTACTCGCGACAGTTCATCACCTCCTGCCCCTGTTGGCTGGAG ATCCTACTCAACAACCACAGAAGCGGACCG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR227576 representing NM_008542
Red=Cloning site Green=Tags(s) KLMFRSKRSGLVRRLWRSRVVPDREEGSGGGGGVDEDGSLGSRAEPAPRAREGGGCSRSEVRSVAPRRPR DAVGPRGAAIAGRRRRTGGLPRPVSESGAGAGGSPLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPR DSGDPQARQSPEPEEGGGPRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVL VPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFTAAADGPTVCCNPYHFSRLCGPESPPP PYSRLSPPDQYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLY AVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTL DAPGGRALVVRKVPPGYSIKVFDFERSGLLQHADAAHGPYDPHSVRISFAKGWGPCYSRQFITSCPCWLE ILLNNHRSGP |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
HindIII-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008542 |
ORF Size | 1500 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2971 bp |
RefSeq ORF | 1488 bp |
Locus ID | 17130 |
UniProt ID | O35182 |
MW | 54.2 kDa |
Gene Summary | Binds to regulatory elements in target promoter regions (By similarity). May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding (By similarity). Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204474 | Smad6 (untagged) - Mouse MAD homolog 6 (Drosophila) (Smad6), (10ug) |
CNY 5,488.00 |
|
MC218743 | Smad6 (untagged) - Mouse MAD homolog 6 (Drosophila) (Smad6), (10ug) |
CNY 6,940.00 |
|
MG227576 | Smad6 (tGFP-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6), (10ug) |
CNY 5,510.00 |
|
MR227576L1 | Lenti ORF clone of Smad6 (Myc-DDK-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6) |
CNY 6,940.00 |
|
MR227576L2 | Lenti ORF clone of Smad6 (mGFP-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6) |
CNY 7,888.00 |
|
MR227576L3 | Lenti ORF clone of Smad6 (Myc-DDK-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6) |
CNY 6,940.00 |
|
MR227576L4 | Lenti ORF clone of Smad6 (mGFP-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6) |
CNY 6,940.00 |