Syne3 (NM_172500) Mouse Tagged Lenti ORF Clone
CAT#: MR227572L4
- LentiORF®
Lenti ORF clone of Syne3 (mGFP-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2
"NM_172500" in other vectors (4)
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CNY 9,690.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | KASH3; nesprin-3 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR227572).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172500 |
ORF Size | 2664 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172500.2, NP_766088.2 |
RefSeq Size | 5083 bp |
RefSeq ORF | 2667 bp |
Locus ID | 212073 |
UniProt ID | Q4FZC9 |
Gene Summary | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222440 | Syne3 (untagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2, (10ug) |
CNY 11,500.00 |
|
MG227572 | Syne3 (tGFP-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik) transcript variant 2, (10ug) |
CNY 8,550.00 |
|
MR227572 | Syne3 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2 |
CNY 6,504.00 |
|
MR227572L3 | Lenti ORF clone of Syne3 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2 |
CNY 9,690.00 |