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Syne3 (NM_172500) Mouse Tagged ORF Clone
CAT＃: MR227572
- TrueORF®
Syne3 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2
ORF Plasmid: tGFP
"NM_172500" in other vectors (4)
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CNY 6,504.00
CNY 7,790.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KASH3; nesprin-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227572 representing NM_172500
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACACGAAGGGGCCCCGAGCGGCCCTGGAGGCAAGGCTTCGAGAGACAGAGTCGCATCGAGTGGG TGTGGCTGCACTGGAGTGAGTACCTGCTGGCCCAGGATGAGTTTTACCGCTGGTTCCAGAAGATGGTGGT CGCACTGGAGCCCCCCGTGGAGCTGCAGCTGGGCTTGAAGGAGAAGCAATGGCAGCTGAGCCACGCCCAG GTGCTGCTGCACAACGTGGACAATCAGGCTGTGCTCCTGGACAGGCTGTTGGAGGAGGCGGGCTCCCTGT TCAGCAGGATCGGAGACCCCAGCGTGGATGAAGATGCCCAGAAGAGGATGAAGGCTGAGTACGATGCCGT GAAGGCCAGAGCCCAGCGCAGGGTGGACCTCCTGGCCCAGGTGGCCCAGGACCATGAGCAGTACCGGGAG GACGTGAATGAGTTCCAGCTGTGGCTGAAGGCGGTGGTGGAGAAGGTGCACAGCTGTCTGGGGCGGAACT GCAAGCTGGCCACAGAACTTCGTCTCTCTACGCTGCAGGACATCGCCAAGGATTTCCCTAGGGGTGAGGA GTCTCTGAAAAGATTGGAGGAACAGGCTGTGGGTGTCATTCAAAACACCTCTCCCTTGGGTGCAGAGAAG ATCTCAGGGGAGCTGGAGGAGATGCGGGGTGTCCTGGAGAAGCTGAGAGTCCTCTGGAAAGAGGAGGAAG GGAGGCTGCGGGGCCTGCTCCAGTCCAGGGGGGACTGTGAGCAGCAGATCCAACAGCTGGAGGCAGAACT GGGAGACTTCAAGAAAAGCCTTCAGAGGCTGGCCCAGGAGGGCTTGGAGCCCACGGTGAAGACAGCCACA GAGGATGAGCTGGTGGCCCAGTGGAGGCTGTTCTCGGGGACTCGGGCTGCACTGGCTTCAGAGGAACCCC GTGTAGACCGGTTACAAACTCAACTGAAGAAACTTGTCACCTTCCCGGACCTGCAGTCACTCTCTGACAG CGTGGTAGCCACCATTCAGGAATACCAAAGTATGAAGGGGAAGAATACCAGGCTCCACAATGCGACCCGG GCAGAGCTGTGGCAGCGTTTCCAGCGGCCCCTAAATGACCTGCAGCTGTGGAAGGCCCTGGCCCAGAGGC TCCTGGACATCACGGCCAGCCTGCCTGACCTGGCCTCCATTCACACCTTTCTACCCCAGATTGAGGCGGC CCTCACGGAAAGCTCTCGCCTGAAAGAGCAGCTGGCGATGCTGCAGCTGAAGACCGACCTGCTGGGCAGC ATCTTTGGCCAGGAGAGAGCAGCCACCCTCCTGGAGCAGGTGACAAGTTCTGTGAGGGACAGAGACCTAC TGCATAACAGCCTTCTTCAGCGGAAGAGCAAACTTCAGAGCCTGCTTGTTCAGCACAAGGACTTTGGGGT GGCTTTTGATCCCCTAAACAGGAAGCTCCTAGACCTCCAGGCCAGGATCCAAGCAGAGAAAGGGCTTCCG AGGGACCTTCCTGGAAAGCAGGTCCAGCTCCTAAGGTTGCAGGGGCTGCAGGAAGAGGGGCTGGATCTGG GGACACAGATCGAGGCTGTGAGGCCTCTTGCCCATGGGAACTCTAAGCACCAGCAGAAAGTAGACCAGAT CTCCTGTGACCAGCAAGCCCTGCAGAGGTCCCTGGAGGATCTCGTGGACAGGTGTCAGCAGAACGTACGG GAACATTGTACCTTCAGTCACAGGCTGTCGGAGCTGCAGCTATGGATCACCATGGCCACACAGACATTAG AGTCACACCAAGGGGATGTGCGTCTGTGGGATGCTGAGTCCCAAGAGGCTGGACTCGAGACGCTGCTGTC TGAAATCCCAGAGAAAGAGGTCCAGGTGTCCCTGCTCCAAGCACTGGGCCAGCTTGTGATGAAGAAGTCT TCCCCAGAAGGGGCAACCATGGTCCAGGAGGAGCTGAGGAAGCTGATGGAGTCTTGGCAGGCCCTGCGGC TGCTAGAGGAGAACATGCTGAGTCTCATGAGAAACCAGCAGCTGCAGAGGACAGAGGTGGACACGGGGAA GAAGCAGGTGTTCACCAACAACATCCCAAAGGCCGGCTTTCTCATCAACCCTCAGGACCCCATTCCCAGG AGACAGCATGGGGCAAACCCACTGGAAGGACACGACCTCCCTGAAGATCATCCCCAGCTCCTGAGGGACT TTGAACAGTGGCTGCAGGCAGAAAACTCCAAGCTACGTAGAATCATCACAATGAGAGTGGCCACAGCCAA GGACTTGAGGACCAGAGAGGTGAAGCTGCAGGAGCTGGAGGCCCGAATCCCAGAAGGCCAGCACCTCTTT GAGAACCTGCTTCGTCTCAGGCCGGCAAGGGACCCCTCCAACGAGCTGGAAGATCTGCGCTACCGGTGGA TGCTGTACAAGTCCAAGCTCAAGGACTCTGGCCACCTGCTGACCGAGAGTTCTCCGGGGGAGCTGACTGC ATTCCAGAAGAGTCGGAGGCAGAAGCGGTGGAGTCCCTGCTCTCTCCTACAGAAAGCATGCCGTGTGGCA CTGCCATTGCAGCTGTTGCTCCTGCTCTTTCTGCTGCTGCTGTTCCTGCTGCCGGCCGGCGAGGAGGAGC GCAGCTGCGCCCTGGCCAACAACTTCGCCCGCTCCTTTGCGCTCATGCTTCGGTACAATGGCCCCCCGCC CACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227572 representing NM_172500
Red=Cloning site Green=Tags(s) MTTRRGPERPWRQGFERQSRIEWVWLHWSEYLLAQDEFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQ VLLHNVDNQAVLLDRLLEEAGSLFSRIGDPSVDEDAQKRMKAEYDAVKARAQRRVDLLAQVAQDHEQYRE DVNEFQLWLKAVVEKVHSCLGRNCKLATELRLSTLQDIAKDFPRGEESLKRLEEQAVGVIQNTSPLGAEK ISGELEEMRGVLEKLRVLWKEEEGRLRGLLQSRGDCEQQIQQLEAELGDFKKSLQRLAQEGLEPTVKTAT EDELVAQWRLFSGTRAALASEEPRVDRLQTQLKKLVTFPDLQSLSDSVVATIQEYQSMKGKNTRLHNATR AELWQRFQRPLNDLQLWKALAQRLLDITASLPDLASIHTFLPQIEAALTESSRLKEQLAMLQLKTDLLGS IFGQERAATLLEQVTSSVRDRDLLHNSLLQRKSKLQSLLVQHKDFGVAFDPLNRKLLDLQARIQAEKGLP RDLPGKQVQLLRLQGLQEEGLDLGTQIEAVRPLAHGNSKHQQKVDQISCDQQALQRSLEDLVDRCQQNVR EHCTFSHRLSELQLWITMATQTLESHQGDVRLWDAESQEAGLETLLSEIPEKEVQVSLLQALGQLVMKKS SPEGATMVQEELRKLMESWQALRLLEENMLSLMRNQQLQRTEVDTGKKQVFTNNIPKAGFLINPQDPIPR RQHGANPLEGHDLPEDHPQLLRDFEQWLQAENSKLRRIITMRVATAKDLRTREVKLQELEARIPEGQHLF ENLLRLRPARDPSNELEDLRYRWMLYKSKLKDSGHLLTESSPGELTAFQKSRRQKRWSPCSLLQKACRVA LPLQLLLLLFLLLLFLLPAGEEERSCALANNFARSFALMLRYNGPPPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172500 |
| ORF Size | 2664 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172500.3, NP_766088.2 |
| RefSeq Size | 5083 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 212073 |
| UniProt ID | Q4FZC9 |
| MW | 102.6 kDa |
| Gene Summary | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222440 | Syne3 (untagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2, (10ug) |
CNY 11,500.00 |
|
| MG227572 | Syne3 (tGFP-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik) transcript variant 2, (10ug) |
CNY 8,550.00 |
|
| MR227572L3 | Lenti ORF clone of Syne3 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2 |
CNY 9,690.00 |
|
| MR227572L4 | Lenti ORF clone of Syne3 (mGFP-tagged) - Mouse RIKEN cDNA 4831426I19 gene (4831426I19Rik), transcript variant 2 |
CNY 9,690.00 |
