United States
Germany
Japan
United Kingdom
China
Itga6 (NM_008397) Mouse Tagged ORF Clone
CAT＃: MR227346
- TrueORF®
Itga6 (Myc-DDK-tagged) - Mouse integrin alpha 6 (Itga6)
ORF Plasmid: tGFP
"NM_008397" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,472.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5033401O05Rik; AI115430; Cd49f; VLA-6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227346 representing NM_008397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCGCGGGCCAGTTGTGCCTGCTCTACCTGTCCGCGGGGCTTCTAGCCCGGCTGGGTACAGCCT TCAACCTGGACACCCGCGAGGACAACGTGATCCGGAAATCGGGGGATCCCGGGAGCCTCTTCGGCTTCTC GCTCGCCATGCACTGGCAGTTGCAGCCGGAGGACAAGCGGCTGTTGCTTGTGGGGGCACCTCGGGCAGAA GCACTCCCGCTGCAGAGGGCGAACAGAACAGGGGGCCTGTACAGCTGTGACATCACCTCCCGGGGACCTT GTACACGGATTGAATTTGATAATGACGCTGATCCTATGTCAGAAAGCAAGGAAGACCAGTGGATGGGAGT CACTGTTCAGAGCCAAGGTCCAGGGGGCAAAGTGGTGACGTGTGCACATCGATATGAGAAACGGCAGCAC GTCAACACGAAGCAGGAGTCGCGGGATATCTTTGGAAGATGTTATGTCCTAAGTCAGAATCTCAGAATTG AAGATGATATGGACGGAGGAGACTGGAGTTTCTGCGATGGCCGGTTGAGAGGCCATGAAAAGTTTGGCTC CTGTCAGCAAGGAGTAGCGGCTACTTTCACTAAGGACTTTCATTACATTGTTTTTGGAGCCCCAGGGACT TACAACTGGAAAGGGATCGTCCGTGTAGAACAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAG ATGGGCCCTATGAAGTTGGTGGAGAGACTGATCATGATGAAAGTCTCGTGCCCGTTCCTGCTAACAGTTA CCTAGGCTTTTCACTGGACTCAGGGAAGGGTATTGTTTCTAAAGATGACATCACTTTTGTGTCTGGTGCT CCAAGAGCCAATCACAGTGGGGCTGTAGTTTTGCTAAAAAGAGACATGAAGTCCGCGCATCTGCTCCCTG AGTATATATTTGACGGAGAAGGCCTGGCTTCCTCGTTTGGCTATGATGTGGCAGTGGTGGACCTCAATGC AGATGGGTGGCAAGACATTGTTATCGGAGCTCCACAGTATTTTGATAGGGATGGTGAAGTCGGGGGTGCA GTTTACGTCTACATTAACCAGCAAGGCAAATGGAGTAATGTGAAGCCGATTCGTCTAAATGGGACCAAAG ACTCGATGTTTGGAATCTCTGTGAAAAATATAGGTGATATTAACCAAGATGGCTATCCAGATATTGCTGT TGGAGCTCCCTATGATGATCTGGGGAAGGTTTTTATCTATCATGGATCCCCGACTGGCATAATTACCAAG CCAACACAGGTTCTCGAGGGGACATCGCCTTACTTCGGCTATTCAATCGCTGGGAATATGGACCTGGATC GGAATTCCTACCCCGACCTTGCTGTGGGCTCCCTCTCAGACTCGGTCACTATTTTCAGATCCCGGCCAGT GATTAACATTCTAAAAACCATCACAGTGACTCCTAACAGAATTGACCTCCGCCAGAAGTCCATGTGTGGC TCACCTAGCGGGATATGCCTCAAGGTTAAAGCCTGTTTTGAATATACTGCGAAACCTTCCGGTTATAACC CTCCAATATCAATTTTGGGTATTCTCGAAGCTGAAAAAGAAAGAAGAAAGTCAGGGTTGTCATCGAGAGT TCAGTTTCGAAACCAAGGTTCCGAGCCAAAGTATACTCAGGAGCTGACCCTGAATCGGCAGAAGCAGCGG GCGTGCATGGAGGAGACCCTCTGGCTGCAGGAGAACATCAGAGACAAGCTGCGTCCCATCCCCATCACGG CTTCTGTGGAGATCCAGGAGCCCAGCTCTCGCCGGCGGGTGAACTCACTCCCCGAAGTTCTTCCCATCCT GAATTCAAATGAAGCCAAAACGGTCCAGACAGATGTCCACTTCTTAAAGGAAGGATGTGGAGACGACAAT GTCTGTAACAGCAACCTTAAGCTAGAGTATAAATTTGGTACCCGAGAAGGAAATCAAGACAAATTCTCTT ACCTTCCAATTCAAAAAGGCATCCCAGAATTAGTCCTAAAAGATCAGAAAGATATAGCTCTGGAAATAAC GGTGACCAACAGCCCTTCGGATCCAAGGAATCCCCGGAAAGATGGCGACGATGCCCATGAAGCCAAACTC ATCGCCACGTTTCCAGACACTCTGACATATTCCGCTTACAGAGAACTGAGGGCTTTCCCTGAGAAGCAGC TGAGCTGTGTGGCCAACCAGAATGGCTCCCAAGCCGACTGTGAGCTCGGAAATCCTTTCAAGAGAAATTC CAGTGTTACTTTCTATCTGATTTTAAGTACAACCGAGGTCACCTTTGACACCACAGATCTGGATATTAAT CTGAAGTTGGAAACAACAAGCAATCAGGATAATTTGGCTCCAATTACAGCGAAGGCAAAAGTGGTTATTG AATTGCTTTTATCGGTCTCCGGAGTCGCTAAGCCTTCGCAGGTGTATTTTGGAGGTACAGTTGTTGGTGA GCAAGCTATGAAATCTGAAGATGAAGTAGGAAGTTTAATAGAGTATGAATTTAGGGTGATTAACTTAGGC AAGCCTCTTAAAAACCTCGGCACAGCAACCTTGAATATACAGTGGCCCAAGGAGATTAGCAATGGCAAAT GGTTGCTTTATTTGATGAAAGTTGAATCCAAAGGTTTGGAGCAGATTGTTTGTGAGCCACACAATGAAAT AAACTACCTGAAGCTGAAGGAGTCTCACAACTCAAGAAAGAAACGGGAACTTCCTGAAAAACAGATAGAT GACAGCAGGAAATTTTCTTTATTTCCTGAAAGAAAATACCAGACTCTCAACTGCAGCGTCAACGTGAGGT GTGTGAACATCAGGTGCCCACTGCGAGGGCTGGACAGCAAGGCCTCTCTCGTTCTTCGTTCCAGGTTGTG GAACAGCACATTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATTCTCCTGAGGGCTTCCATAGAT GTCACCGCTGCTGCTCAGAATATCAAGCTCCCTCACGCGGGCACTCAGGTTCGAGTGACGGTGTTTCCCT CAAAGACTGTAGCTCAGTATTCAGGAGTAGCTTGGTGGATCATCCTCCTGGCTGTTCTTGCCGGGATTCT GATGCTGGCTCTATTAGTGTTTTTACTGTGGAAGTGTGGCTTCTTCAAGAGAAATAAGAAAGATCATTAC GATGCCACCTATCACAAGGCTGAGATCCATACTCAGCCGTCTGATAAAGAGAGGCTTACTTCCGATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227346 representing NM_008397
Red=Cloning site Green=Tags(s) MAVAGQLCLLYLSAGLLARLGTAFNLDTREDNVIRKSGDPGSLFGFSLAMHWQLQPEDKRLLLVGAPRAE ALPLQRANRTGGLYSCDITSRGPCTRIEFDNDADPMSESKEDQWMGVTVQSQGPGGKVVTCAHRYEKRQH VNTKQESRDIFGRCYVLSQNLRIEDDMDGGDWSFCDGRLRGHEKFGSCQQGVAATFTKDFHYIVFGAPGT YNWKGIVRVEQKNNTFFDMNIFEDGPYEVGGETDHDESLVPVPANSYLGFSLDSGKGIVSKDDITFVSGA PRANHSGAVVLLKRDMKSAHLLPEYIFDGEGLASSFGYDVAVVDLNADGWQDIVIGAPQYFDRDGEVGGA VYVYINQQGKWSNVKPIRLNGTKDSMFGISVKNIGDINQDGYPDIAVGAPYDDLGKVFIYHGSPTGIITK PTQVLEGTSPYFGYSIAGNMDLDRNSYPDLAVGSLSDSVTIFRSRPVINILKTITVTPNRIDLRQKSMCG SPSGICLKVKACFEYTAKPSGYNPPISILGILEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLNRQKQR ACMEETLWLQENIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSNEAKTVQTDVHFLKEGCGDDN VCNSNLKLEYKFGTREGNQDKFSYLPIQKGIPELVLKDQKDIALEITVTNSPSDPRNPRKDGDDAHEAKL IATFPDTLTYSAYRELRAFPEKQLSCVANQNGSQADCELGNPFKRNSSVTFYLILSTTEVTFDTTDLDIN LKLETTSNQDNLAPITAKAKVVIELLLSVSGVAKPSQVYFGGTVVGEQAMKSEDEVGSLIEYEFRVINLG KPLKNLGTATLNIQWPKEISNGKWLLYLMKVESKGLEQIVCEPHNEINYLKLKESHNSRKKRELPEKQID DSRKFSLFPERKYQTLNCSVNVRCVNIRCPLRGLDSKASLVLRSRLWNSTFLEEYSKLNYLDILLRASID VTAAAQNIKLPHAGTQVRVTVFPSKTVAQYSGVAWWIILLAVLAGILMLALLVFLLWKCGFFKRNKKDHY DATYHKAEIHTQPSDKERLTSDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008397 |
| ORF Size | 3219 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008397.4, NP_032423.2 |
| RefSeq Size | 4205 bp |
| RefSeq ORF | 3222 bp |
| Locus ID | 16403 |
| MW | 120.1 kDa |
| Gene Summary | This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 4 to bind laminin and to form the main component of hemidesmosomes, which mediate attachment of epithelia to basement membranes. In mouse, deficiency of this gene is associated with absence of hemidesmosomes, severe skin blistering, and early post-natal death. In humans mutations of this gene are associated with epidermolysis bullosa. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223447 | Itga6 (untagged) - Mouse integrin alpha 6 (Itga6), (10ug) |
CNY 13,780.00 |
|
| MG227346 | Itga6 (tGFP-tagged) - Mouse integrin alpha 6 (Itga6), (10ug) |
CNY 9,072.00 |
|
| MR227346L3 | Lenti ORF clone of Itga6 (Myc-DDK-tagged) - Mouse integrin alpha 6 (Itga6) |
CNY 9,872.00 |
|
| MR227346L4 | Lenti ORF clone of Itga6 (mGFP-tagged) - Mouse integrin alpha 6 (Itga6) |
CNY 10,260.00 |
