Mib1 (NM_144860) Mouse Tagged Lenti ORF Clone
CAT#: MR227236L3
- LentiORF®
Lenti ORF clone of Mib1 (Myc-DDK-tagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1)
"NM_144860" in other vectors (4)
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CNY 10,260.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DIP-1; E430019M12Rik; Mib; mKIAA1323 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR227236).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_144860 |
ORF Size | 3021 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144860.2, NP_659109.2 |
RefSeq Size | 3793 bp |
RefSeq ORF | 3021 bp |
Locus ID | 225164 |
UniProt ID | Q80SY4 |
Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation (By similarity). Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223183 | Mib1 (untagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1), (10ug) |
CNY 13,020.00 |
|
MG227236 | Mib1 (tGFP-tagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1), (10ug) |
CNY 9,220.00 |
|
MR227236 | Mib1 (Myc-DDK-tagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1) |
CNY 7,016.00 |
|
MR227236L4 | Lenti ORF clone of Mib1 (mGFP-tagged) - Mouse mindbomb homolog 1 (Drosophila) (Mib1) |
CNY 10,260.00 |