Trip13 (NM_027182) Mouse Tagged ORF Clone

CAT#: MR227183

  • TrueORF®

Trip13 (Myc-DDK-tagged) - Mouse thyroid hormone receptor interactor 13 (Trip13)

ORF Plasmid: DDK tGFP



  "NM_027182" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2410002G23Rik; D13Ertd328e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR227183 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGGCGGTGGGCGACCTGAAGCAAGCGCTTCCCTGTGTTGCCGAATCGCCCGCGGTCCATGTGG
AGGTTCTGCAGCGCAGCGGAAGCACTGCAAAAAAAGAAGATATAAAGTCGAGTGTTTACAGGCTGCTCAA
CCGGCATAATATTGTGTTTGGAGATTACGTCTGGACTGAGTTTGATGATCCTTTTCTAAGCAGAAATGTT
CAGTCAGTGTCTATTGTTGACACAGAACTAAAGGCTAAAGACCCTCAGCCCATTGATCTGAGTGCATGCA
CCATTGCACTTCACATCTTCCAGCTGAATGAAGAAGGCCCCAGCAGTGAGAATTTGGATGAAGAAACAGA
AAATATAATTGCAGCAAGTCACTGGGTTCTGCCTGCAGCTGAATTTCATGGACTTTGGGATAGCCTCGTG
TATGATGTGGAGGTCAAATCACATCTCCTTGATTATGTGATGACCACCGTACTCTTCTCAGACAAGAACG
TGGACAGCAACCTCATCACCTGGAACCGGGTGGTGCTGCTGCACGGTCCTCCGGGTACTGGAAAAACATC
CCTTTGTAAGGCATTAGCCCAAAAACTGACCATCAGACTGTCGAGCAGGTACCGGTATGGCCAGTTGATT
GAAATAAACAGCCACAGCCTATTTTCTAAGTGGTTTTCAGAAAGTGGCAAATTGGTAACTAAGATGTTCC
AGAAGATTCAGGACTTGATTGATGATAAGGAAGCTTTGGTGTTTGTTCTGATTGATGAGGTGGAGAGTCT
CACAGCTGCTCGAAATGCTTGCAGGGCAGGCGCAGAGCCATCAGATGCTATCCGAGTAGTCAATGCTGTG
TTGACTCAGATTGACCAGATTAAAAGGCATTCCAATGTGGTGATTCTGACCACTTCCAACATCACTGAGA
AGATTGATGTGGCTTTCGTGGATAGAGCTGACATCAAGCAATACATTGGCCCCCCCTCTGCAGCAGCCAT
CTTCAAAATCTACCTGTCTTGTTTAGAAGAACTGATGAAGTGCCAGATCATATATCCTCGTCAGCAGCTG
TTGACCCTTCGGGAGCTGGAAATGATTGGCTTCATTGAAAATAATGTGTCAAAGTTGAGCCTCCTTTTGA
GTGAAATTTCAAGGAAGAGTGAGGGCCTCAGTGGCCGGGTCTTGAGGAAACTTCCTTTCCTGGCTCATGC
TCTCTACATCCAGGCCCCCAGCGTCACCATCGAGGGTTTCCTCCAGGCCCTATCTCTGGCAGTGGACAAA
CAGTTTGAGGAGAAAAAGAAACTTTCAGCTTATGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227183 protein sequence
Red=Cloning site Green=Tags(s)

MDEAVGDLKQALPCVAESPAVHVEVLQRSGSTAKKEDIKSSVYRLLNRHNIVFGDYVWTEFDDPFLSRNV
QSVSIVDTELKAKDPQPIDLSACTIALHIFQLNEEGPSSENLDEETENIIAASHWVLPAAEFHGLWDSLV
YDVEVKSHLLDYVMTTVLFSDKNVDSNLITWNRVVLLHGPPGTGKTSLCKALAQKLTIRLSSRYRYGQLI
EINSHSLFSKWFSESGKLVTKMFQKIQDLIDDKEALVFVLIDEVESLTAARNACRAGAEPSDAIRVVNAV
LTQIDQIKRHSNVVILTTSNITEKIDVAFVDRADIKQYIGPPSAAAIFKIYLSCLEELMKCQIIYPRQQL
LTLRELEMIGFIENNVSKLSLLLSEISRKSEGLSGRVLRKLPFLAHALYIQAPSVTIEGFLQALSLAVDK
QFEEKKKLSAYV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_027182
ORF Size 1299 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_027182.2, NP_081458.1
RefSeq Size 2267 bp
RefSeq ORF 1299 bp
Locus ID 69716
UniProt ID Q3UA06
MW 48.4 kDa
Gene Summary Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (PubMed:17696610, PubMed:19851446, PubMed:20711356). Plays a role in mitotic spindle assembly checkpoint (SAC) activation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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