Gsn (NM_146120) Mouse Tagged ORF Clone

CAT＃: MR227060

TrueORF^®

Gsn (Myc-DDK-tagged) - Mouse gelsolin (Gsn), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_146120" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,712.00

CNY 6,840.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

TA363004

GSN Antibody - middle region

CNY 4,628.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227060 representing NM_146120 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCGTACCGCTCTTCACTGCTCTGCGCGCTGCTCCTCCTGGCGCTGTGCGCACTGTCGCCTTCCC ATGCAGCCACCACATCACGGGGCAGGGCCCAAGAGAGGGCGCCCCAAAGTCGGGTGTCTGAGGCGCGGCC CAGCACTATGGTGGTGGAGCACCCCGAATTCCTGAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGT GTGGAGAAGTTTGACCTGGTGCCTGTGCCCCCCAACCTCTATGGAGACTTCTTCACGGGTGATGCCTATG TCATCCTAAAGACTGTGCAGCTGAGGAATGGGAATCTGCAGTATGACCTCCACTATTGGCTGGGCAATGA ATGCAGCCAGGATGAGAGCGGGGCTGCTGCCATCTTTACTGTGCAACTGGATGACTACCTGAACGGCCGG GCTGTACAGCACCGTGAGGTTCAGGGCTTTGAGTCGTCCACCTTCTCCGGCTACTTCAAGTCTGGACTTA AGTACAAGAAAGGAGGTGTGGCATCTGGATTCAAACACGTGGTACCCAATGAGGTGGTGGTCCAGAGGCT CTTCCAGGTCAAAGGACGCCGTGTAGTCCGTGCTACTGAGGTACCTGTGTCCTGGGACAGTTTCAACAAT GGCGACTGCTTCATTCTGGACCTGGGAAACAATATCTATCAGTGGTGTGGCTCTGGCAGCAACAAATTTG AAAGGCTGAAGGCCACACAGGTGTCCAAGGGCATCCGGGACAACGAGAGGAGTGGCCGTGCTCAAGTACA CGTGTCTGAAGAGGGAGGAGAGCCAGAAGCCATGCTGCAGGTGCTGGGCCCCAAGCCAGCTCTGCCTGAA GGTACCGAGGACACAGCCAAGGAAGATGCAGCCAACCGAAGGCTGGCCAAGCTCTACAAGGTCTCCAACG GTGCAGGTAGCATGTCAGTCTCCCTAGTGGCTGATGAGAACCCCTTCGCCCAGGGCGCCCTGAGATCTGA GGACTGCTTCATCCTGGACCATGGCAGAGATGGGAAAATCTTTGTTTGGAAAGGCAAGCAGGCCAACATG GAGGAGCGGAAGGCTGCCCTCAAAACAGCCTCTGACTTCATCTCCAAGATGCAGTACCCCAGGCAGACCC AGGTTTCAGTTCTCCCAGAGGGCGGTGAGACCCCTCTCTTTAAGCAGTTCTTCAAGAACTGGCGGGACCC AGACCAGACAGACGGCCCCGGCCTGGGCTACCTCTCCAGCCACATTGCCAACGTGGAGCGCGTACCTTTC GATGCTGCTACGCTGCACACCTCCACCGCCATGGCCGCTCAGCACGGCATGGATGATGATGGAACTGGCC AGAAACAGATCTGGAGAATTGAAGGTTCCAACAAGGTGCCAGTGGACCCTGCCACATACGGGCAGTTCTA TGGAGGCGACAGCTACATCATTCTGTACAACTACCGCCACGGTGGCCGCCAGGGACAGATCATCTACAAC TGGCAGGGTGCCCAGTCTACCCAGGATGAGGTTGCTGCTTCTGCCATCCTGACTGCCCAGCTGGATGAGG AGCTGGGAGGAACTCCTGTCCAGAGCCGAGTGGTCCAAGGCAAAGAGCCTGCACACCTCATGAGCTTGTT TGGCGGGAAGCCCATGATCATCTACAAGGGTGGCACCTCCCGTGATGGTGGGCAGACAGCTCCTGCCAGT ATCCGCCTCTTCCAAGTGCGAGCCAGCAGCTCTGGAGCCACCAGGGCTGTGGAGGTGATGCCTAAGTCTG GTGCTCTGAACTCCAACGATGCCTTTGTGCTGAAAACCCCCTCCGCTGCCTACCTGTGGGTGGGCGCAGG AGCCAGTGAGGCGGAGAAGACCGGCGCCCAGGAGCTTCTGAAGGTGCTTCGGTCCCAGCATGTGCAGGTG GAAGAAGGCAGTGAGCCAGATGCCTTCTGGGAGGCTCTGGGCGGGAAGACTGCCTACCGCACATCCCCCA GGCTTAAGGACAAGAAGATGGATGCCCATCCTCCTCGACTCTTTGCCTGCTCCAACAGGATCGGACGCTT TGTGATCGAAGAGGTTCCTGGCGAGCTTATGCAGGAAGACCTGGCTACTGATGACGTCATGCTCCTGGAC ACCTGGGACCAGGTCTTTGTCTGGGTTGGAAAAGACTCCCAGGAAGAGGAAAAGACGGAAGCCTTGACTT CTGCTAAGCGGTACATCGAGACAGATCCAGCAAATCGGGACAGGCGGACCCCCATCACAGTCGTTAGGCA GGGCTTTGAGCCTCCTTCCTTCGTGGGCTGGTTCCTCGGCTGGGATGACAACTACTGGTCGGTGGATCCT TTGGACCGGGCCTTGGCTGAGCTGGCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227060 representing NM_146120 Red=Cloning site Green=Tags(s) MAPYRSSLLCALLLLALCALSPSHAATTSRGRAQERAPQSRVSEARPSTMVVEHPEFLKAGKEPGLQIWR VEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGR AVQHREVQGFESSTFSGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWDSFNN GDCFILDLGNNIYQWCGSGSNKFERLKATQVSKGIRDNERSGRAQVHVSEEGGEPEAMLQVLGPKPALPE GTEDTAKEDAANRRLAKLYKVSNGAGSMSVSLVADENPFAQGALRSEDCFILDHGRDGKIFVWKGKQANM EERKAALKTASDFISKMQYPRQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGPGLGYLSSHIANVERVPF DAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYN WQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSRDGGQTAPAS IRLFQVRASSSGATRAVEVMPKSGALNSNDAFVLKTPSAAYLWVGAGASEAEKTGAQELLKVLRSQHVQV EEGSEPDAFWEALGGKTAYRTSPRLKDKKMDAHPPRLFACSNRIGRFVIEEVPGELMQEDLATDDVMLLD TWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVRQGFEPPSFVGWFLGWDDNYWSVDP LDRALAELAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_146120
ORF Size	2340 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_146120.4, NP_666232.2
RefSeq Size	2663 bp
RefSeq ORF	2343 bp
Locus ID	227753
UniProt ID	P13020
MW	86.4 kDa
Gene Summary	Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221593	Gsn (untagged) - Mouse gelsolin (Gsn), transcript variant 1, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,720.00
MG227060	Gsn (tGFP-tagged) - Mouse gelsolin (Gsn), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,510.00
MR227060L3	Lenti ORF clone of Gsn (Myc-DDK-tagged) - Mouse gelsolin (Gsn), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,740.00
MR227060L4	Lenti ORF clone of Gsn (mGFP-tagged) - Mouse gelsolin (Gsn), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,740.00

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