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Map3k5 (NM_008580) Mouse Tagged ORF Clone
CAT＃: MR227032
- TrueORF®
Map3k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5)
ORF Plasmid: tGFP
"NM_008580" in other vectors (4)
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CNY 9,448.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 7420452D20Rik; ASK; ASK1; MAPKKK5; Mekk5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGAAGCCGGCGAGGGCATCACTTTCTCCGTGCCGCCCTTTGCGTCCGTGGGCTTTTGCACCA TCCCGGAGGGCGGCAGCTGCCGGAGGGGAGGAGGAGCGGCGACGGCGGCGGAGGGAGAGCCGAGCCTGCA GCCCTTGCTTGTGCCGCCGCCGCCGCCACCGCCGGGCAGTTTCTGGAACGTGGAGAGCGCGGCGGCTCCC GGCACCAGCTGCCCTACCACCGCCCCCGGGAGCAGCGCCACCCGAGGCCGGGGCAACTCTGGCAGCGGGG GCGGCCGGCGTACCACCGTGGCGTATGTGATCAATGAGGCGAGCCAGGGGCAGCTGGTGGTGGCGGAGAG CGAGGCCCTGCAGAGCCTGCGGGAGGCGTGCGAGGCGGTGGGCGCCACCCTGGAGACCCTGCATTTCGGG AAGCTGGACTTCGGGGAAACCGCCGTGCTGGACCGTTTTTACAACGCAGATATTGCTGTGGTGGAGATGA GCGACGCCTTCCGGCAGCCTTCCTTGTTTTACCATCTTGGAGTGCGAGAAAGCTTCAGTATGGCCAACAA CATCATCCTCTACTGCGATACTAATTCCGATTCACTCCAGTCCCTGAAGGAAATTATTTGCCAGAAGAAT ACTGTGTGCACCGGGAACTACACCTTCATCCCCTACATGGTGACACCACACAACAAGGTCTACTGCTGTG ACAGCAGCTTCATGAAGGGCCTGACTGAGCTCATGCAGCCTAACTTCGAGCTCCTGCTGGGGCCCATCTG CTTACCCCTCGTGGACCGTTTTGTTCAGCTTTTGAAGGTGGCGCAAGCGAGTTCCAGCCAGTACTTCCGG GAATCCATACTCAGCGATATCAGGAAAGCACGGAACCTGTACACTGGTAAAGAATTGGCAGCTGAATTGG CGAGGATCCGGCAACGGGTAGACAATATTGAAGTTTTGACAGCAGACATTGTTATCAACCTGTTGCTTTC CTACAGAGACATCCAGGACTATGACTCCATAGTGAAGCTGGTAGAGACGTTAGAAAAACTTCCAACCTTC GACTTGGCCTCGCACCACCACGTGAAGTTTCATTACGCATTTGCACTGAACAGGAGAAATCTCCCTGGAG ACAGAGCAAAAGCTCTTGACATCATGATCCCCATGGTACAAAGTGAAGAGCAAGTTGCTTCGGATATGTA TTGTCTCGTTGGCCGAATCTACAAAGATATGTTTTTGGATTCAAATTTCACTGACACGGAAAGCAGGGAC CATGGAGCATCTTGGTTCAAAAAGGCATTTGAGTCTGAGCCAACACTACAGTCAGGAATTAATTATGCAG TTCTCTTGCTGGCGGCTGGGCACCAGTTTGAATCTTCTTTTGAGCTTCGGAAAGTTGGAGTGAAGCTGAG TAGCCTTCTGGGTAAAAAGGGGAACTTGGAAAAACTCCAGAGCTACTGGGAAGTCGGATTTTTTCTGGGA GCCAGTGTCCTGGCCAACGACCACCTCAGGGTCATTCAGGCATCCGAGAAGCTGTTCAGACTGAAGACAC CAGCGTGGTACCTCAAGTCTATCGTGGAGACCATTCTGATATATAAACACTTTGTGAAACTGACCACAGA GCAGCCCTCGGCTAAGCAGGAACTCGTGGACTTCTGGATGGATTTCCTGGTCGAGGCCACAAAAACAGAT GTTACTGTTGTCAGGTTCCCAGTATTAATATTAGAGCCAACCAAAATCTATCAGCCTTCTTACCTGTCTA TCAATAATGAAGTTGAGGAGAAGACAATATCTATTTGGCATGTTCTCCCTGATGACAAGAAAGGTATACA CGAGTGGAACTTCGGTGCTTCCTCTGTCAGGGGAGTGAGCATCTCTAAGTTTGAAGAACGATGCTGTTTC CTTTATGTGCTTCACAATTCTGATGACTTCCAAATCTACTTCTGCACAGAACTTCACTGTAAAAGGTTTT TTGAAATGGTGAATACCATCACGGAAGAGAAGGGGAGAGGCGCGGAGGACGGAGACTGTGAGGGTGACTC CCTGGAGTATGACTACGAATACGATGAAAATGGAGACAGAGTTGTGTTAGGGAAGGGCACTTATGGGATC GTCTATGCAGGACGAGACCTGAGCAACCAGGTCAGAATTGCTATTAAGGAAATCCCAGAGAGAGACAGCA GATACTCTCAGCCCCTGCATGAAGAAATCGCCCTGCACAAGCATCTGAAACACAAAAACATTGTCCAGTA TCTGGGCTCCTTCAGTGAGAACGGCTTCATCAAAATCTTCATGGAGCAAGTCCCAGGAGGAAGCCTCTCT GCTCTCCTTCGTTCAAAATGGGGCCCATTAAAGGACAACGAACAGACGATTGGCTTCTATACGAAGCAGA TACTGGAAGGATTAAAATACCTTCACGACAATCAGATAGTCCACCGGGATATAAAGGGTGACAATGTATT GATTAATACCTACAGTGGTGTGCTCAAGATCTCTGACTTCGGGACATCCAAGAGGCTTGCTGGCATAAAC CCATGTACCGAAACCTTTACAGGTACCCTTCAGTATATGGCGCCAGAAATAATCGATAAAGGACCACGAG GCTATGGAAAGGCCGCCGACATTTGGTCACTGGGCTGCACAATCATCGAAATGGCTACTGGAAAACCACC ATTTTATGAACTAGGAGAGCCACAGGCAGCCATGTTCAAGGTGGGGATGTTTAAGGTCCACCCGGAGATC CCAGAGTCCATGTCGGCGGAGGCCAAGGCGTTCATACTGAAGTGTTTTGAACCAGACCCTGACAAGAGAG CCTGTGCTAATGACTTGCTTATTGATGAGTTCTTAAAAGTGTCCAGCAAAAAGAAAAAGACACAACCCAA ACTTTCAGCTCTCTCGACTGGATCGAATGAGTATCTTAGAAGCATCTCCCTGCCGGTCCCTGTCCTGGTA GAAGACACCAGCAGCAGTAGCGAGTATGGCTCTGTTTCTCCTGATACAGAGCTGAAGGCGGACCCCTTCT CCTTCAAAGCCAGAGCTAAGTCCTGTGGAGAAAAGGACGGGAAAGGGATACGGACATTGTTCCTGGGCAT TCCAGATGAAAATTTTGAAGATCACAGTGCGCCCCCATCTCCTGAAGAGAAAGACTCGGGCTTTTTTATG CTGAGAAAGGATAGTGAGAGGCGAGCTACCCTTCACAGAATCCTGACCGAAGACCAGGACAAGGTTGTAA GGAACCTAATGGAATCTCTGGCCCAGGGTGCTGAAGAGCCTAAACTAAAGTGGGAACACATCACAACCCT CATCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGAAAAATCATAGCCACTACATTGTCCAAGCTC AAGCTGGAGCTGGACTTCGACAGCCACGGCATCAGCCAGGTGCAGGTGGTCCTCTTTGGCTTCCAGGATG CGGTCAATAAAGTTCTTCGGAATCATAACATCAAGCCACACTGGATGTTTGCCCTGGACAGCATCATCCG AAAGGCTGTGCAGACTGCAATTACCATTCTGGTTCCAGAGTTGAGGCCACATTTTAGCCTTGCATCAGAG AGCGACACAGCTGATCCGGAAGACCTGGATGTCGAAGATGAACACGAGGAATTGTCTTCAAATCAGACAG TCCGACGGCCTCAGGCCATCACTGAGGACGCTGTGGCTACCTCGGGGGTGAGCACGCTAAGCTCCACTGT GTCCCATGACTCCCAGAATGCACACCGGTCGCTGAACGTGCAGCTTGGAAGGATGAAGATTGAAACTAAT AGGTTACTTGAAGAGCTGGTTCGGAAAGAGAGAGAGTTACAGGCACTCCTCCATCAAGCCATTGAGGAAA AAGACCAAGAAATTAGGCACCTGAAGCTCAAGTCCCAGCCCATAGATATCCCTGGGTTTCCTGTGTGCCA CCTGAACTCTCCTGGCACGACCACGGAAGATTCTGAACTTCCTGGCTGGCTGAGAGAAAATGGAGCTGAT GAAGACACTATAAGTCGGTTTCTGGCTGAGGATTACACGCTAGTGGATGTTCTTTACTACGTCACACGTG ATGATCTGAAGTGCCTCAGACTAAGGGGCGGGATGCTGTGCACGCTGTGGAAGGCCATCATTGACTTTCG GAACAAATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227032 protein sequence
Red=Cloning site Green=Tags(s) MGTEAGEGITFSVPPFASVGFCTIPEGGSCRRGGGAATAAEGEPSLQPLLVPPPPPPPGSFWNVESAAAP GTSCPTTAPGSSATRGRGNSGSGGGRRTTVAYVINEASQGQLVVAESEALQSLREACEAVGATLETLHFG KLDFGETAVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKN TVCTGNYTFIPYMVTPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFVQLLKVAQASSSQYFR ESILSDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTF DLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEEQVASDMYCLVGRIYKDMFLDSNFTDTESRD HGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLG ASVLANDHLRVIQASEKLFRLKTPAWYLKSIVETILIYKHFVKLTTEQPSAKQELVDFWMDFLVEATKTD VTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFGASSVRGVSISKFEERCCF LYVLHNSDDFQIYFCTELHCKRFFEMVNTITEEKGRGAEDGDCEGDSLEYDYEYDENGDRVVLGKGTYGI VYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLS ALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGIN PCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEI PESMSAEAKAFILKCFEPDPDKRACANDLLIDEFLKVSSKKKKTQPKLSALSTGSNEYLRSISLPVPVLV EDTSSSSEYGSVSPDTELKADPFSFKARAKSCGEKDGKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFM LRKDSERRATLHRILTEDQDKVVRNLMESLAQGAEEPKLKWEHITTLISSLREFVRSTDRKIIATTLSKL KLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASE SDTADPEDLDVEDEHEELSSNQTVRRPQAITEDAVATSGVSTLSSTVSHDSQNAHRSLNVQLGRMKIETN RLLEELVRKERELQALLHQAIEEKDQEIRHLKLKSQPIDIPGFPVCHLNSPGTTTEDSELPGWLRENGAD EDTISRFLAEDYTLVDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008580 |
| ORF Size | 4143 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_008580.3, NM_008580.4, NP_032606.4 |
| RefSeq Size | 5393 bp |
| RefSeq ORF | 4143 bp |
| Locus ID | 26408 |
| UniProt ID | O35099 |
| MW | 154.5 kDa |
| Gene Summary | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224370 | Map3k5 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5), (10ug) |
CNY 17,670.00 |
|
| MG227032 | Map3k5 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5), (10ug) |
CNY 11,880.00 |
|
| MR227032L3 | Lenti ORF clone of Map3k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5) |
CNY 12,730.00 |
|
| MR227032L4 | Lenti ORF clone of Map3k5 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 5 (Map3k5) |
CNY 12,730.00 |
