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Trpc4 (NM_016984) Mouse Tagged ORF Clone
CAT＃: MR226912
- TrueORF®
Trpc4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4)
ORF Plasmid: tGFP
"NM_016984" in other vectors (4)
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CNY 10,696.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCE1; STRPC4; Trp4; Trrp4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226912 representing NM_016984
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTTTTATATGGGGAGAGATTAAACAG ATGTGGGATGGCGGACTCCAGGATTACATCCATGACTGGTGGAATCTAATGGACTTTGTGATGAACTCCT TGTATCTGGCAACAATCTCCTTGAAGATTGTCGCGTTTGTAAAGTACAGTGCTCTGAACCCACGGGAATC ATGGGACATGTGGCACCCCACCCTGGTGGCAGAGGCATTATTTGCTATTGCAAACATCTTCAGTTCCCTC CGCCTGATCTCTCTGTTCACTGCCAATTCTCACCTGGGGCCTCTGCAGATATCTCTGGGAAGGATGCTTC TGGACATCCTGAAGTTCTTGTTCATCTACTGCCTCGTGCTGCTAGCTTTTGCAAATGGCCTAAATCAGCT GTACTTTTACTATGAAGAAACAAAGGGGCTAAGCTGCAAAGGCATCCGGTGCGAGAAACAGAACAACGCG TTTTCCACGTTATTCGAGACACTACAGTCCCTGTTTTGGTCAATATTTGGACTCATCAATCTCTATGTTA CCAATGTCAAAGCCCAGCACGAGTTCACTGAGTTTGTTGGGGCCACCATGTTTGGCACATATAATGTCAT CTCTCTGGTTGTCCTGCTGAACATGTTAATTGCTATGATGAATAATTCTTACCAACTAATTGCCGACCAT GCAGATATAGAATGGAAATTTGCTCGAACAAAGCTTTGGATGAGCTACTTTGAAGAAGGAGGTACCCTGC CTACACCTTTCAATGTCATCCCAAGCCCCAAGTCCCTGTGGTACCTGGTCAAGTGGATATGGACACACTT ATGTAAGAAAAAAATGAGAAGGAAGCCAGAAAGCTTCGGGACAATTGGGCGGCGTGCTGCTGATAACTTG AGAAGACATCACCAATACCAAGAGGTGATGAGGAACCTGGTGAAGCGGTACGTGGCAGCCATGATCAGAG AGGCAAAAACCGAAGAAGGCTTGACGGAGGAGAATGTTAAGGAACTAAAGCAAGACATTTCTAGCTTCCG CTTCGAAGTTCTGGGATTGCTCAGAGGAAGCAAGCTCTCTACAATACAGTCAGCCAACGCGGCGAGTTCA GCGGACTCCGACGAGAAGAGCCAGAGCGAAGGTAATGGCAAGGACAAGAGAAAGAATCTCAGCCTCTTTG ATTTAACCACTCTGATCCACCCGCGGTCGGCAGCCATTGCCTCCGAGAGACATAACCTAAGCAATGGTTC CGCCCTGGTGGTGCAGGAGCCGCCCAGGGAGAAGCAGAGGAAAGTGAATTTTGTGGCTGATATCAAAAAC TTCGGGTTATTTCATAGACGGTCAAAACAAAATGCTGCTGAGCAAAACGCAAACCAAATCTTCTCTGTTT CAGAAGAAATTACTCGTCAACAGGCGGCAGGAGCACTTGAGCGAAATATCGAACTGGAATCCAAAGGATT AGCTTCACGGGGTGACCGCAGCATTCCTGGTCTCAATGAACAGTGTGTGCTAGTAGACCATAGAGAAAGG AATACGGACACTTTGGGTTTACAGGTAGGCAAGAGAGTGTGCTCCACCTTCAAGTCGGAGAAGGTGGTGG TGGAAGACACCGTCCCTATTATACCAAAGGAGAAACACGCCCACGAGGAGGACTCGAGCATAGACTATGA CTTAAGCCCCACGGACACAGCTGCCCACGAAGATTATGTGACCACAAGATTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR226912 representing NM_016984
Red=Cloning site Green=Tags(s) MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLG RTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFT PDITPIILAAHTNNYEIIKLLVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIA LSSEDPFLTAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNSLIEE QSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIA PKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQ MWDGGLQDYIHDWWNLMDFVMNSLYLATISLKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSL RLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLSCKGIRCEKQNNA FSTLFETLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADH ADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLVKWIWTHLCKKKMRRKPESFGTIGRRAADNL RRHHQYQEVMRNLVKRYVAAMIREAKTEEGLTEENVKELKQDISSFRFEVLGLLRGSKLSTIQSANAASS ADSDEKSQSEGNGKDKRKNLSLFDLTTLIHPRSAAIASERHNLSNGSALVVQEPPREKQRKVNFVADIKN FGLFHRRSKQNAAEQNANQIFSVSEEITRQQAAGALERNIELESKGLASRGDRSIPGLNEQCVLVDHRER NTDTLGLQVGKRVCSTFKSEKVVVEDTVPIIPKEKHAHEEDSSIDYDLSPTDTAAHEDYVTTRL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016984 |
| ORF Size | 2922 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016984.3, NP_058680.1 |
| RefSeq Size | 3490 bp |
| RefSeq ORF | 2925 bp |
| Locus ID | 22066 |
| UniProt ID | Q9QUQ5 |
| MW | 112 kDa |
| Gene Summary | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222901 | Trpc4 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4), (10ug) |
CNY 10,712.00 |
|
| MG226912 | Trpc4 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 4 (Trpc4), (10ug) |
CNY 12,296.00 |
|
| MR226912L3 | Lenti ORF clone of Trpc4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4) |
CNY 11,590.00 |
|
| MR226912L4 | Lenti ORF clone of Trpc4 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 (Trpc4) |
CNY 11,590.00 |
