Neu1 (NM_010893) Mouse Tagged ORF Clone
CAT#: MR226903
- TrueORF®
Neu1 (Myc-DDK-tagged) - Mouse neuraminidase 1 (Neu1)
ORF Plasmid: tGFP
"NM_010893" in other vectors (3)
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CNY 3,656.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AA407268; AA407316; Aglp; Apl; Bat-7; Bat7; G9; Map-2; Neu; Neu-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226903 representing NM_010893
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGGCAGACCCGACCAGACCCCGGGGACCGCTGAGCTATTGGGCGGGCCGTCGGGGTCAGGGGC TCGCAGCGATCTTCCTGCTCCTGGTGTCCGCGGCGGAATCCGAGGCCAGGGCAGAGGATGACTTCAGCCT GGTGCAGCCGCTGGTGACCATGGAGCAGCTGCTGTGGGTGAGCGGGAAGCAGATCGGCTCTGTAGACACT TTCCGCATCCCGCTCATCACAGCCACCCCTCGGGGCACGCTCCTGGCCTTCGCTGAGGCCAGGAAAAAAT CTGCATCCGATGAGGGGGCCAAGTTCATCGCCATGAGGAGGTCCACGGACCAGGGTAGCACGTGGTCCTC TACAGCCTTCATCGTAGACGATGGGGAGGCCTCCGATGGCCTGAACCTGGGCGCTGTGGTGAACGATGTA GACACAGGGATAGTGTTCCTTATCTATACCCTCTGTGCTCACAAGGTCAACTGCCAGGTGGCCTCTACCA TGTTGGTTTGGAGTAAGGACGACGGCATTTCCTGGAGCCCACCCCGGAATCTCTCTGTGGATATTGGCAC AGAGATGTTTGCCCCTGGACCTGGCTCAGGCATTCAGAAACAGCGGGAGCCTGGGAAGGGCCGGCTCATT GTGTGTGGACACGGGACGCTGGAGCGAGATGGGGTCTTCTGTCTCCTCAGTGATGACCACGGTGCCTCCT GGCACTACGGCACTGGAGTGAGCGGCATTCCCTTTGGCCAGCCCAAACACGATCACGATTTCAACCCCGA CGAGTGCCAGCCCTACGAGCTTCCAGATGGCTCGGTCATCATCAACGCCCGGAACCAGAATAACTACCAT TGCCGCTGCAGGATCGTCCTCCGCAGCTATGACGCCTGTGACACCCTCAGGCCCCGGGATGTGACCTTCG ACCCTGAGCTCGTGGACCCTGTGGTAGCTGCAGGAGCACTAGCCACCAGCTCCGGCATTGTCTTCTTCTC CAATCCAGCCCACCCTGAGTTCCGAGTGAACCTGACCCTGCGCTGGAGTTTCAGCAATGGTACATCCTGG CAGAAGGAGAGGGTCCAGGTGTGGCCGGGACCCAGCGGCTACTCGTCCCTGACAGCCCTGGAAAACAGCA CGGATGGAAAGAAGCAGCCCCCGCAGCTGTTCGTTCTGTACGAGAAAGGCCTGAACCGGTACACCGAGAG CATCTCCATGGTCAAAATCAGCGTCTACGGCACGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226903 representing NM_010893
Red=Cloning site Green=Tags(s) MVGADPTRPRGPLSYWAGRRGQGLAAIFLLLVSAAESEARAEDDFSLVQPLVTMEQLLWVSGKQIGSVDT FRIPLITATPRGTLLAFAEARKKSASDEGAKFIAMRRSTDQGSTWSSTAFIVDDGEASDGLNLGAVVNDV DTGIVFLIYTLCAHKVNCQVASTMLVWSKDDGISWSPPRNLSVDIGTEMFAPGPGSGIQKQREPGKGRLI VCGHGTLERDGVFCLLSDDHGASWHYGTGVSGIPFGQPKHDHDFNPDECQPYELPDGSVIINARNQNNYH CRCRIVLRSYDACDTLRPRDVTFDPELVDPVVAAGALATSSGIVFFSNPAHPEFRVNLTLRWSFSNGTSW QKERVQVWPGPSGYSSLTALENSTDGKKQPPQLFVLYEKGLNRYTESISMVKISVYGTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010893 |
ORF Size | 1227 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010893.3, NP_035023.3 |
RefSeq Size | 3850 bp |
RefSeq ORF | 1230 bp |
Locus ID | 18010 |
UniProt ID | O35657 |
MW | 45 kDa |
Gene Summary | Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Interplay Between Sialic Acids, Siglec-E, and Neu1 Regulates MyD88- and TRIF-Dependent Pathways for TLR4-Activation During Leishmania donovani Infection
,Karmakar, J;Mandal, C;,
Frontiers in immunology
,PubMed ID 33763070
[NEU1]
|
Modulation of TLR4 Sialylation Mediated by a Sialidase Neu1 and Impairment of Its Signaling in Leishmania donovani Infected Macrophages
,Karmakar, J;Roy, S;Mandal, C;,
Front Immunol
,PubMed ID 31649671
[NEU1]
|
Neu1 sialidase interacts with perilipin 1 on lipid droplets and inhibits lipolysis in 3T3-L1 adipocytes
,Natori, Y;Nasui, M;Kihara-Negishi, F;,
Genes Cells
,PubMed ID 28429532
[NEU1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226903 | Neu1 (tGFP-tagged) - Mouse neuraminidase 1 (Neu1), (10ug) |
CNY 5,256.00 |
|
MR226903L3 | Lenti ORF clone of Neu1 (Myc-DDK-tagged) - Mouse neuraminidase 1 (Neu1) |
CNY 4,750.00 |
|
MR226903L4 | Lenti ORF clone of Neu1 (mGFP-tagged) - Mouse neuraminidase 1 (Neu1) |
CNY 4,750.00 |