Tjp1 (NM_001163574) Mouse Tagged ORF Clone
CAT#: MR226867
- TrueORF®
Tjp1 (Myc-DDK-tagged) - Mouse tight junction protein 1 (Tjp1), transcript variant 2
ORF Plasmid: tGFP
"NM_001163574" in other vectors (2)
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CNY 16,820.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ZO1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226867 representing NM_001163574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGGTGTACAGGAAGGAGCCGTATTCTGAAGAAATGATGA GACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCCAGGGCAGAGGCCAGATAAAGAGCCAAATCT AGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCCAGCAGAGACCTTGAGCAGCCGTCATACAGG TATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACTATGACCATCGCCTACGGTTTGAAGATCGAA TCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAAACAGCCCTACCAACCTCGGCCTTTTGAGAA TCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAAGAGGCTTCAGAACGAGGTTATTTCCAGCGT TTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACACGCTATGAGCAGCTGCCTCGAACCTCTACTC TACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCACAACAGGTACAGGCCAGAGGCACAGCCCTA TTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTACTTTGACCAGTACCCGCGAAGTTATGAGCAA GTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACGAGCCTCTCCATGGTGCTGCAGTTGTCCCTC CTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCCCTCGGCTACCAAACCACAGCCTCCACCCCC AACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCACAGTCTGTGCTCACCAGAGTCAAAATGTTT GAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATGTGAATGACACTGCCAGCTTTAAGCCTCCGG AAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCCTAAACCTGTCCCTCAGAGTCAGTTTAGTGA GCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAACCTCAAGTGAAGCCACCCGAAGATATTGTT CGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATTACCGGAAACAGCTCTCCTACTTTGACCGAA GAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGGCCATCACTCAGAGCCTGCTAAGCCAGTCCA TTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAGGGAAAACCCGAAACTGATGCTGTGGATAGA TCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGCCTCCTCCTCCTCCGTTGCCCTCACAGTACA GCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACATACATTCCAAGGGCGCCCAGGGTGAAGGCAA CTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAACCAGACCCACCCCCATCTCAGAGTAAACCA GCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGACTTTCTATCCGCAGAAAAGTTTCCCAGACA AAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCATCACTCCGGTGTACAATCGATTCACACCAAA GCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTTGAAAGTCCGAAGTTCAACCATAATCTTCTG CCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAACTCCCACTTCCCCAAAAACTCTTATGAAAG CTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGTTGAAACTTTCTCTGTTCACACAGATAAGCC TAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCTGTCCCTGTGAGTCCTTCAGCTGTGGAAGAA GATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTCGTGGCATTTTTAACAGCAATGGTGGTGTGT TGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACAAGGAGCCATTCCTGAAGGAATTGAGCAAGA AATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCACCTTTAGATAAAGAGAAAGGTGAAACTCTG CTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGTTCCTGAAGCCCGTGGAGCTACGCTTGCCAC ACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATCCGACTCCTCGTCGGGTGACCC TAAAACCTGGCAAAACAAGTGTCTTCCTGGAGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTC CTGATTGACCACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226867 representing NM_001163574
Red=Cloning site Green=Tags(s) MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQ ENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPEPVSDNEDDSYDEEVHDPR AGRGALANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKE ISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHS ANASERDDISEIQSLASDHSGRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPG AISTPVKHVDDHPPKAVEEVTVEKNEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGALPNSAHEDGILRP SMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDL PKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAI RIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKF PAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQ DKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFT TTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHT SDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRI DSPGLKPASQQVYRKEPYSEEMMRQNHILKQPALGHPGQRPDKEPNLAYEPQLPYIEKQASRDLEQPSYR YEVSSYTDQFSRNYDHRLRFEDRIPTYEDQWSYYDDKQPYQPRPFENQHPRDLDSRQHPEEASERGYFQR FEEPAPLSYDSRTRYEQLPRTSTLRHEEQPAPAYEVHNRYRPEAQPYSSTGPKSSEPKQYFDQYPRSYEQ VPPPGFTSKTGHYEPLHGAAVVPPLIPSSQQKPEVLPSATKPQPPPPTLTEEEEDPAMKPQSVLTRVKMF ENKRSASLENKKDVNDTASFKPPEVASKPPGASLAGPKPVPQSQFSEHDKTLYRLPEPQKPQVKPPEDIV RSNHYDPEEDEEYYRKQLSYFDRRSFESKPSAHLPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAVDR SFSEKRYDPAQATPPPPPLPSQYSQPAPPLSSSSLHIHSKGAQGEGNSVSLDFQNSYMSKPDPPPSQSKP ATFRPPTREDPPQTFYPQKSFPDKAPVNGAEQTQKTITPVYNRFTPKPYTSSARPFERKFESPKFNHNLL PSETVHKPELSSKTPTSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQMNNISTMPKAVPVSPSAVEE DEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETL LSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSV LIDHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163574 |
ORF Size | 5055 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163574.1, NP_001157046.1 |
RefSeq Size | 6891 bp |
RefSeq ORF | 5058 bp |
Locus ID | 21872 |
MW | 189.3 kDa |
Gene Summary | Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
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