Tjp1 (NM_001163574) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ZO1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224825 representing NM_001163574 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGCCAGGGCCGCGGCCGCTAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAGCACACAG TGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAATTGCAATCTCTGGTGGAAGAGATAATCCTCATTT TCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGTGTTAAAAGGAGGGCCAGCTGAAGGACAGCTACAG GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAACGTTGAACATGCTTTTGCTGTTCAGC AGCTAAGGAAGAGTGGGAAAAACGCAAAAATTACTATCCGAAGGAAGAAGAAAGTTCAGATCCCTGTAAG TCACCCAGATCCTGAGCCGGTGTCTGATAATGAAGACGATAGTTATGACGAAGAAGTGCATGACCCAAGA GCTGGCCGCGGTGCTTTAGCGAACAGAAGGAGCGAGAAGAGCTGGGCAAGGGATAGGAGTGCAAGCAGGG AGAGGAGCCTGTCCCCTCGCTCGGACAGGCGGTCCGTGGCCTCCAGTCAGCCCGCAAAGCCCACCAAGGT CACACTGGTGAAGTCTCGGAAAAATGAAGAATATGGTCTTCGATTGGCCAGCCATATATTTGTAAAGGAA ATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAACATTCAAGAAGGGGATGTTGTCTTGAAGATAAATG GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAAACACTGATAGAAAGGTCTAAAGGCAAGTTAAA AATGGTAGTGCAAAGAGATGAGCGGGCTACCTTACTGAACGTCCCTGACCTTTCGGATAGTATCCATTCT GCTAATGCCTCGGAAAGAGATGACATTTCAGAAATTCAGTCACTAGCATCAGACCATTCAGGTCGCTCGC ATGACAGGCCACCCCGCCGCAGCCAGTCACGATCTCCTGACCAACGTTCAGAGCCCTCCGATCATTCCAC GCAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCGGAGCAGAGAGGAAGAGCGAATGTCTAAACCTGGG GCCATCTCAACTCCTGTAAAACATGTAGACGATCATCCACCCAAAGCAGTGGAAGAAGTTACAGTTGAGA AAAATGAGAAGCAGACGCCCACTCTTCCAGAACCGAAACCTGTGTATGCTCAAGTTGGACAACCAGATGT GGATTTACCCGTCAGCCCTTCTGATGGTGCTCTGCCTAATTCAGCTCATGAAGACGGGATACTTAGGCCC AGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGACTAGCTGGTGGAAATGATGTCG GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAAT TCTCAGGGTGAACAATGTAGATTTCACAAATATCATAAGGGAAGAGGCCGTCCTTTTCCTCCTTGACCTC CCTAAAGGTGAAGAAGTGACCATACTGGCTCAGAAGAAGAAGGACGTTTATCGCCGCATTGTAGAATCAG ATGTAGGAGATTCATTCTATATTAGAACGCATTTTGAATATGAAAAAGAATCTCCTTACGGACTTAGTTT TAACAAAGGAGAGGTGTTCCGGGTCGTGGATACTTTATACAATGGAAAGCTGGGCTCTTGGCTTGCCATT CGAATTGGCAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAACAGTTAG CCAGTGTACAGTACACACTTCCAAAGACAGCGGGTGGTGATCGGGCAGACTTCTGGAGGTTTCGAGGTCT TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGACTTGTCAGCTCAGCCAGTTCAAACTAAGTTC CCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGATTCCTAAGACCTGTAACCATCTTTGGACCAATAG CTGATGTTGCCAGAGAAAAGTTGGCAAGAGAGGAGCCAGATATCTATCAGATTGCAAAAAGTGAACCACG AGATGCTGGGACTGACCATCGTAGCTCTGGCATCATTCGCCTTCATACAATAAAGCAAATCATAGATCAG GATAAACATGCTTTATTAGATGTAACGCCAAATGCGGTTGATCGTCTTAATTATGCGCAGTGGTATCCAA TTGTTGTGTTCCTTAACCCTGACTCTAAGCAAGGTGTAAAAACAATGAGGATGAGGTTGTGTCCGGAGTC TCGGAAAAGCGCCAGGAAGCTATATGAACGCTCTCATAAGCTTCGTAAGAACAATCACCATCTCTTCACA ACTACAATTAACTTAAACTCAATGAATGATGGTTGGTACGGTGCCCTGAAAGAAGCGATTCAGCAGCAAC AGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA CGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGAGTACTCAATGTATAGCACGGACAGTAGACACACT TCTGACTATGAAGACACAGATACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG ATGAGGTGGGGACTCCCCCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGG AATGCATCATGAAAACCAGACATACCCTCCTTACTCACCACAAGCGCAGCCACAAGCTATTCATAGAATA GACTCCCCTGGACTTAAGCCAGCCTCTCAACAGGTGTACAGGAAGGAGCCGTATTCTGAAGAAATGATGA GACAAAACCATATTTTAAAACAACCAGCTCTTGGTCACCCAGGGCAGAGGCCAGATAAAGAGCCAAATCT AGCCTATGAACCCCAACTTCCATATATAGAAAAACAAGCCAGCAGAGACCTTGAGCAGCCGTCATACAGG TATGAGGTCTCAAGCTACACAGACCAGTTTTCTCGGAACTATGACCATCGCCTACGGTTTGAAGATCGAA TCCCTACCTATGAAGACCAGTGGTCATATTATGATGACAAACAGCCCTACCAACCTCGGCCTTTTGAGAA TCAGCATCCCCGAGACCTGGACTCCAGACAACATCCCGAAGAGGCTTCAGAACGAGGTTATTTCCAGCGT TTTGAAGAGCCAGCCCCTCTGTCGTACGACAGTAGAACACGCTATGAGCAGCTGCCTCGAACCTCTACTC TACGACATGAAGAGCAGCCAGCCCCTGCATATGAGGTGCACAACAGGTACAGGCCAGAGGCACAGCCCTA TTCTTCAACAGGCCCTAAGTCATCTGAGCCCAAGCAGTACTTTGACCAGTACCCGCGAAGTTATGAGCAA GTTCCACCACCAGGCTTTACCTCCAAAACAGGCCATTACGAGCCTCTCCATGGTGCTGCAGTTGTCCCTC CTCTGATACCTTCCTCTCAACAAAAGCCAGAAGTCCTGCCCTCGGCTACCAAACCACAGCCTCCACCCCC AACCCTAACTGAGGAGGAGGAGGATCCAGCAATGAAACCACAGTCTGTGCTCACCAGAGTCAAAATGTTT GAAAACAAAAGATCTGCGTCTTTGGAGAACAAGAAAGATGTGAATGACACTGCCAGCTTTAAGCCTCCGG AAGTAGCATCTAAACCTCCAGGTGCTTCTCTTGCTGGCCCTAAACCTGTCCCTCAGAGTCAGTTTAGTGA GCACGACAAAACGCTCTACAGGCTCCCAGAGCCTCAGAAACCTCAAGTGAAGCCACCCGAAGATATTGTT CGATCAAATCATTACGACCCTGAAGAGGATGAAGAATATTACCGGAAACAGCTCTCCTACTTTGACCGAA GAAGTTTTGAGAGCAAGCCTTCTGCACATCTTCCTGCTGGCCATCACTCAGAGCCTGCTAAGCCAGTCCA TTCTCAGAGCCAGCCCAACTTCTCTAGTTACTCTTCAAAGGGAAAACCCGAAACTGATGCTGTGGATAGA TCATTCAGTGAGAAACGTTATGATCCAGCCCAGGCCACGCCTCCTCCTCCTCCGTTGCCCTCACAGTACA GCCAGCCAGCTCCACCTCTGTCCAGCTCTTCTCTCCACATACATTCCAAGGGCGCCCAGGGTGAAGGCAA CTCAGTATCATTGGATTTTCAGAACTCATATATGTCCAAACCAGACCCACCCCCATCTCAGAGTAAACCA GCAACTTTCAGACCACCAACTCGAGAAGACCCCCCTCAGACTTTCTATCCGCAGAAAAGTTTCCCAGACA AAGCTCCAGTTAACGGAGCTGAGCAGACTCAGAAAACCATCACTCCGGTGTACAATCGATTCACACCAAA GCCGTACACAAGTTCTGCCCGGCCATTTGAACGCAAATTTGAAAGTCCGAAGTTCAACCATAATCTTCTG CCAAGTGAAACTGTACATAAACCTGAATTGTCTTCAAAAACTCCCACTTCCCCAAAAACTCTTATGAAAG CTCATAGTTCAACACAGCCTCCAGAGTTTGACAGTGGAGTTGAAACTTTCTCTGTTCACACAGATAAGCC TAAATATCAAATGAATAATATCAGCACCATGCCTAAAGCTGTCCCTGTGAGTCCTTCAGCTGTGGAAGAA GATGAAGATGAGGATGGTCATACTGTAGTGGCTACAGCTCGTGGCATTTTTAACAGCAATGGTGGTGTGT TGAGTTCCATAGAAACCGGTGTTAGTATAATTATCCCACAAGGAGCCATTCCTGAAGGAATTGAGCAAGA AATCTATTTCAAAGTCTGCAGAGACAATAGCATTCTCCCACCTTTAGATAAAGAGAAAGGTGAAACTCTG CTGAGCCCCCTAGTGATGTGTGGGCCCCATGGCCTCAAGTTCCTGAAGCCCGTGGAGCTACGCTTGCCAC ACTGTGCGTCCATGACTCCTGACGGTTGGTCTTTTGCTCTAAAATCATCCGACTCCTCGTCGGGTGACCC TAAAACCTGGCAAAACAAGTGTCTTCCTGGAGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTCTGTC CTGATTGACCACTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163574
Insert Size	5058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163574.1, NP_001157046.1
RefSeq Size	6891 bp
RefSeq ORF	5058 bp
Locus ID	21872
Gene Summary	Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame coding exon in the middle region but has an additional in-frame segment in the 3' coding region, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1.

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