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Usp36 (NM_001033528) Mouse Tagged ORF Clone
CAT＃: MR226452
- TrueORF®
Usp36 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 36 (Usp36)
ORF Plasmid: tGFP
"NM_001033528" in other vectors (4)
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CNY 7,648.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700002L06Rik; mKIAA1453 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226452 representing NM_001033528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTATAGTCGATAAGCTGAAGGAGGCTCTCAAGCCTGGCCGCAAGGACTCGGCCGAGGATGGGGACC TGGGCAGGCTGCTGGCAGCCTCTGCCAAGAAGGTGCTTCTGCAGAGGATTGAGTTTGAGCCGGCCAGCAA GAGCTTCTCCTACCAGCTGGAGTCCTTGAAGAGCAAATACGTACTGTTGAGCGCCAGGGCTGAGGGAGCC AGTCGCCACAGGAGCGGAGATGAACTCCAGGCCAGGAAACCAGGCACCGAGCGTGTGTCTGGAAGTGGAG GCGACGGAGTCCCTGCCCCGCAGAAAGTGCTCTTCCCTGTGGAGCGTCTGTCCCTGAGGTGGGAACGTGT TTTCCGAGTTGGCGCAGGGCTGCACAACCTGGGTAACACCTGCTTCCTCAACTCCACCATCCAGTGCTTG ACCTACACACCACCTCTGGCCAACTACTTGCTCTCCAAGGAGCACGCACGGAGCTGTCACCAAGGGGGCT TCTGCATGCTGTGTCTCATGCAAAACCACATGGTCCAGGCCTTTGCCAACAGCGGCAATGCTATCAAGCC CGTCTCCTTCATCAGAGACCTGAAAAAGATTGCCCGGCACTTCCGGTTCGGGAACCAAGAGGATGCACAC GAATTCCTGCGGTACACCATTGATGCGATGCAGAAGGCCTGCTTGAATGGCTACGCTAAGTTGGATCGGC AGACACAGGCTACTACCCTGGTGCATCAGATCTTTGGAGGCTATCTCAGGTCCCGAGTGAAGTGCTCTGT GTGCAAGAGTGTCTCAGACACATACGATCCCTACTTGGACATAGCACTGGAGATCCGGCAAGCTGCAAAT ATTGTGCGCGCTCTGGAACTTTTTGTGAAGTCAGATGTCCTGAGTGGAGAGAACGCCTATATGTGTGCTA AGTGTAAGAAGAAGGTTCCAGCCAGCAAGCGCTTCACCATCCATAGAACATCCAATGTCCTGACTCTGTC CCTCAAGCGCTTCGCCAACTTTAGTGGGGGGAAGATCACCAAGGATGTTGGCTATCCAGAGTTCCTGAAC ATCCGTCCATACATGTCGCAGAGCAGCGGTGATCCTGTCATGTATGGGCTCTATGCTGTCCTGGTGCACT CAGGCTACAGCTGTCACGCTGGGCACTACTACTGCTATGTGAAGGCCAGCAATGGACAGTGGTACCAGAT GAACGACTCCTTGGTCCATTCCAGCAATGTCAAGGTGGTTCTGAACCAGCAGGCCTACGTGCTCTTCTAT CTGCGGATTCCAGGCTCTAAGAAAAGTCCTGAGGGCCCTGTCTCCAGAGTGGGCGCCACTCTCCCTAGCC GTCCCAAAGTTGTTCCAGAGCACTCCAAGAAGAGCCCTGGCAATGGGGTTGTTCCCTCCCCGCTGATGGC AAAGCGACAAGACTCTGTAATGATGAGAAAGCTCCCAGCCCCTGAGGAGGTTGGCGTACCTGTGTCCAGA AATGGCTCCCTTCCTGGCCTGAAGTTACAGAACGGATGTGCTCCTGCCAAGACACCTGCCGGGTCCCCGT CCCCAAGACTCACGCCCACGCCCACTCATATGCCCACCATTCTGGATGAGCCCGGAAAGAAGGTGAAGAA GTCAGCCCCACTGCAGTCTCTCACCACATCTCCAACCACTTCTCAGGGCTCTCCTGGGACCGGCGAGTCC CGGAGCCAGCGGCCCGGCTCCTGGGCAAGCAGAGACACCATCTTCTCTACCTCCCCGAAGCTCCTGGCCA GAGCCATTACTAACGGGCACAGGCTGAAGGGCGAAGGCAGTGGTGTGGACCTGGAGAAGGGGGATTCAAG CAGCTCCAGCCCCGAGCACTCTGCCAGCAGTGACCCTGCCAAGGCCCCGCAGACCGCAGAGAGCAGAGCT GCGCATGCCTGTGATTCTCAGGGAACAAACTGTCCCACCGCTGGCCATCCCAAAGCGCTGTTGAATGGAG TAGATGCCAAGATGGTGAAGTTGAAGTCCCCGGCCCTGAGCAGCACCACAACCGAGCCCACAAGCCTCAT GTCTCCTCCACCAGCCAAAAAACTGGCCCTGTCAGCCAAGAAGGCCAGCACCCTGCGGAGGGCGACCGGC AATGACATCGGTTCACCTTCCCCCTCAGCATTCTGCGACCTCACCTCCCCCATGAAAGCCACCCACCCCG TCGTTGCCTCCACTGGGCCTGTCAGTAAGACCAGGACTGCTGCACCTGCCCCCCGACCGTCCACCCACCC CCACTCTGCATCTCTGTCCAGTAGTAGTGCCAAGCCCTTAGGGACATCAGAGCCACAGAGCTGCCGCCCC TCTGCCTGGACACCCCTGCCTCAGGTCAATGGACACTTCACGTCCCACTTACACCAACTGCCAGAGGCCA GCGAGGCCCTACACAGCCCCTCCAAAAAGAGGAAAAAGACCCCTAATGGAGATCCCCAGAGACTGGGCAT CGACACGCTCCTCCCACAGTGCCTCAGGGGAGCACCTGCAGCAGCACGCAGGAAGAGAAAGAAGAGGTGC TCGGAGGGTGAGGGTGCCACAGCTCCCAAGCAGGAAGGCCAATTTCAGGACCAGTCTTGGAGTTCCGGGA GCCAGAAGGAAGAGGGTACGCAGCCCCAGGTAAATGGCCATCAAGTGAGTCACATTCTGGACAGTTACCA TGTGAGCAGCAGGAAAAGGAGGAAGAGGAAAAGATCGGAAGGACTCAGCCAGGAAGCCACCCCGTCGCAG GACCTAATTCAGCACAGCTGCTCCCCTGTAGACCACAGTGAGCCTGAGGCCAGGACAGAGTTGCAGAAGA AGAAGAAGAAGAAAAGAAGGAAAAGAAAGCCCGAGCCACAGCAGGATGAAGAGAGCAAGCACCCAGGAGA CCAGAGGAGCCCAAGGCCCAGTGTCACCCCAGTCCCTGCGTTGAGTGTGAATGGCCATCTTCCTAGTGAC TGCTTGGGGCTGGGACAAGCACCTCTCGTTACCTGGAACAGAGATCAGGAACCCGATGTGGTCCAGGCGT TGCTTCAGGACTCATCTGATAAGGCTTATGGGAAAAAAGTTTTGACCTGGGATGGCGAGCCTTCAGCCAT CAGTCAGGACGCCATCAAAGACAGCAGACTAGCCCGGACCCAGACCGTGGTTGACGACTGGGATGAAGAG TTTGATCGAGGGAAGGAAAAGAAAATTAAAAAGTTCAAGAGAGAAAAGAAGAGAAACTTCAATGCCTTCC AGAAGCTTCAGAGTAGGCGCAACTTTTGGTCTGTGACGCATCCTGCTAAGGTGGCCAGCCTCAGCTATCG CCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226452 representing NM_001033528
Red=Cloning site Green=Tags(s) MPIVDKLKEALKPGRKDSAEDGDLGRLLAASAKKVLLQRIEFEPASKSFSYQLESLKSKYVLLSARAEGA SRHRSGDELQARKPGTERVSGSGGDGVPAPQKVLFPVERLSLRWERVFRVGAGLHNLGNTCFLNSTIQCL TYTPPLANYLLSKEHARSCHQGGFCMLCLMQNHMVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAH EFLRYTIDAMQKACLNGYAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDIALEIRQAAN IVRALELFVKSDVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLN IRPYMSQSSGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFY LRIPGSKKSPEGPVSRVGATLPSRPKVVPEHSKKSPGNGVVPSPLMAKRQDSVMMRKLPAPEEVGVPVSR NGSLPGLKLQNGCAPAKTPAGSPSPRLTPTPTHMPTILDEPGKKVKKSAPLQSLTTSPTTSQGSPGTGES RSQRPGSWASRDTIFSTSPKLLARAITNGHRLKGEGSGVDLEKGDSSSSSPEHSASSDPAKAPQTAESRA AHACDSQGTNCPTAGHPKALLNGVDAKMVKLKSPALSSTTTEPTSLMSPPPAKKLALSAKKASTLRRATG NDIGSPSPSAFCDLTSPMKATHPVVASTGPVSKTRTAAPAPRPSTHPHSASLSSSSAKPLGTSEPQSCRP SAWTPLPQVNGHFTSHLHQLPEASEALHSPSKKRKKTPNGDPQRLGIDTLLPQCLRGAPAAARRKRKKRC SEGEGATAPKQEGQFQDQSWSSGSQKEEGTQPQVNGHQVSHILDSYHVSSRKRRKRKRSEGLSQEATPSQ DLIQHSCSPVDHSEPEARTELQKKKKKKRRKRKPEPQQDEESKHPGDQRSPRPSVTPVPALSVNGHLPSD CLGLGQAPLVTWNRDQEPDVVQALLQDSSDKAYGKKVLTWDGEPSAISQDAIKDSRLARTQTVVDDWDEE FDRGKEKKIKKFKREKKRNFNAFQKLQSRRNFWSVTHPAKVASLSYRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033528 |
| ORF Size | 3294 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033528.1, NP_001028700.1 |
| RefSeq Size | 5609 bp |
| RefSeq ORF | 3297 bp |
| Locus ID | 72344 |
| UniProt ID | B1AQJ2 |
| MW | 120.4 kDa |
| Gene Summary | Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634). Function as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223559 | Usp36 (untagged) - Mouse ubiquitin specific peptidase 36 (Usp36), (10ug) |
CNY 14,060.00 |
|
| MG226452 | Usp36 (tGFP-tagged) - Mouse ubiquitin specific peptidase 36 (Usp36), (10ug) |
CNY 9,220.00 |
|
| MR226452L3 | Lenti ORF clone of Usp36 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 36 (Usp36) |
CNY 10,260.00 |
|
| MR226452L4 | Lenti ORF clone of Usp36 (mGFP-tagged) - Mouse ubiquitin specific peptidase 36 (Usp36) |
CNY 10,260.00 |
