Araf (NM_001159645) Mouse Tagged ORF Clone
CAT#: MR226088
- TrueORF®
Araf (Myc-DDK-tagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 2
ORF Plasmid: tGFP
"NM_001159645" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1200013E08Rik; A-Raf; Araf1; AW495444 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR226088 representing NM_001159645
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCACCACGAGGCCCTCCTGTTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGT ACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCCGGGAAGGCATGAGTGTCTATGACTCTTTGGACAA GGCCCTGAAGGTGCGGGGTCTCAACCAGGACTGCTGTGTGGTCTACAGGCTCATCAAAGGAAGAAAGACA GTCACTGCCTGGGACACAGCCATTGCGCCTCTGGATGGTGAGGAGCTCATTGTGGAGGTCCTAGAAGATG TGCCACTGACCATGCACAATTTTGTACGGAAGACATTCTTCAGCCTGGCCTTCTGTGACTTCTGCCTTAA GTTTCTGTTCCATGGATTCCGCTGCCAGACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC CCCACCGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATCTGTCTGGAG GCTCCAGGCAGCAGGAGGCTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226088 representing NM_001159645
Red=Cloning site Green=Tags(s) MEPPRGPPVSGAEPSRAVGTVKVYLPNKQRTVVTVREGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV PTVCVDMSTNRRQFYHSIQDLSGGSRQQEAPSNLSVNELLTPQGPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001159645 |
ORF Size | 558 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159645.1, NP_001153117.1 |
RefSeq Size | 1571 bp |
RefSeq ORF | 561 bp |
Locus ID | 11836 |
UniProt ID | P04627 |
MW | 21.3 kDa |
Gene Summary | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208144 | Araf (untagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
MG226088 | Araf (tGFP-tagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
MR226088L3 | Lenti ORF clone of Araf (Myc-DDK-tagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 2 |
CNY 4,750.00 |
|
MR226088L4 | Lenti ORF clone of Araf (mGFP-tagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 2 |
CNY 4,750.00 |