Lct (NM_001081078) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gm100; LAC; LPH; Lphl
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225984 representing NM_001081078 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCCTTGGACAGCACTCTTCCTCAGTACCTTCCTCCTGGGTCTTTCATGCCAGGGGAATGACT GGGAATCTGACAGAAATTTTATTTCAGCTGCTGGTCCTCTAACAAATGACTTAGTGCACAATCTGAACCA TCCGCTGGGAAAGCAGGGCTCTGATTTGGTAGCTGGTGATACTGACATCTATGTCTGCCAGCAGCCACTG CCCTCCTTCTTGCCTCAGTACTTCAGCAGTCTTCGGGCCAGCCAGGTTACCCATTACAAAGTACTTCTGT CATGGGCACAACTTCTCCCAAAAGGAAGCTCCAAGAATCCAGACCAGGAAGCAGTGCAGTGCTACCGACA ACTCCTCCAGTCCCTGAAGGATGCCCAGCTTCAGCCCATGGTGGTCCTGTTCCACCAGATGCCACCTACC AGCACCATCCAGAGAGACGGGGGCTTTGCTGACCTCTTTGCTGATTATGCCACATTGGCTTTTCAGTCCT TTGGGGACCTTGTTGAGATCTGGTTCACCTTCAGTGACTTGGAGAAAGTGATCATGGGTCTTCCCCACCA GCATTTGAAAGCTTCTGGTCTCCAAACCCTCAGCGATGCCCACAGGAAAGCCTTTGACGTTTACCACAGA AAGTACTCTTCTCAGGGAGGAAAGCTGTCTGTGGTCCTGAAGGCTGAAGACCTCCCCAAGCTTCTGCCAG ACCCATCCTCAGCGGCTCTCGTCAAGGGCTCCGTGGATTTCCTCTCTCTTGATTTGTCTTATGACTGCCA AAGTATATCGACTCTACCACAGAAGCTGAGTGAATTGCAGAACCTTGAGCCCAAAGTGAAAGTTTTCGTC TACACCCTAAAACCTCAGGACTGCCCATCGACTGGAATGAGCCCCGCCTCACTGCTCTTCAGACTCCTGG AAGCCATAAATAAAGACCAAGTCCAGACTGTTGGGTTTGATGTCAGTGCCTTCCTGAGCTGTACGTCCAG TTCTGAGGAGAGCCCATCTTGTTCTTTAACCTACAGTAGCCTGGCCCTTCAGAGCGAGCAGCAGCATGAG ACAGTTGTGTCCTCATTCCCTGGGTCGGCCTACCAGAGAGTCTGGGCAGCCTTGGCTAACCAGTCTAGGG AGGAAAGGGACGCTTTCCTGCAAGATGTATTTCCAGAAGGGTTCCTCTGGGGCGTATCCACGGGAGCCTT TAACGTGGAAGGAGGCTGGGCCGAAGGTGGCAGAGGGCCAAGCATCTGGGACCACTACAGTAACCTGAAT GCTGCGGAGAGCCAAGCGACAGCAAAGGTGGCCAGTGACAGTTACCACAAACCAGTTTCTGATGTGGCCC TCCTCCGAGGCCTCAGGGCTGACGTGTACAAATTCTCCATCTCCTGGTCCCGCATCTTCCCTTTCGGGCA GAGGACCAGCCCCAACCTCCAAGGTGTTGCCTACTACAACAAACTGATTGACAGCCTGCTGGACTCCCAG GTGGAGCCCATGGCTACGCTGTTCCACTGGGACCTCCCCCAGGCCCTGCAGGAGCAAGGCGGGTGGCAGA ACGAGAGTGTGGTGGATGCCTTCCTGGACTACGCCGCCTTCTGCTTCTCCACCTTTGGGGACCGTGTGAA GCTGTGGGTGACCTTCCATGAGCCCTGGGTGATAAGCTATGCAGGCTACGGCACCGGGCAGCATGCGCCA GCCATCTCTGACCCAGGAGTGGCTTCCTTTAAGGTGGCTCACCTGATCCTCAAGGCGCATGCCAGAACTT GGCATCACTACAACTATCACCATCGCCAAAAGCAGCAGGGACGCGTGGGCATTGTGCTCAATTCAGACTG GGCGGAGCCCCTGGACGGGAAGAGCCCGCAGGACCTTGCTGCTGCCGAGCGCTACCTGCACTTCATGCTA GGCTGGTTTGCACACCCCATCTTCATAGATGGGGACTACCCTGCTGCCCTTCGGGCCCAGATCCAGCACA CAAACCAGCAGTGTGGCCGTCCTCTGGCCCAACTCCCAGAGTTCACTGAAGCAGAGAAGCGGCTCCTGAA AGGCTCTGCGGATTTCCTAGGTCTGTCTCATTACACATCCCGCCTCATCAGCAAGGCCGGGCAGCAGACC TGCATCCCCAGCTATGATAACATCGGAGGCTTCTCCCAGCACGTAGATCCCAAGTGGCCCCAGACCGCAT CGCCCTGGATCCGAGTGGTGCCCTGGGGCATAAGGAGGCTGCTTCGGTTCGCTTCCCTGGAATACACGAA AGGAAAACTTCCAATCTTCTTGGCTGGAAATGGCATGCCAATAGGGGAAGGGTCGGATCTGTTTGATGAC TCAATGAGAGTAAACTACTTAAACTTGTACATCAACGAGGTGCTCAAGGCTGTCAAAGAGGATTCAGTGG ATGTCCGTTCATACATTGCTCGTTCCCTCATTGATGGCTATGAAGGACCCCTGGGCTATAGCCAGAGGTT TGGGCTATACCATGTCAACTTCAATGACAGCAGCAGGCCACGAACTCCCAGGAAATCTGCCTACTTCTTC ACCAGCATCATTGAAAAGAACGGTTTTCCTGCCAAGAAAGTCAAAAGAAACCCACTACCCTTGAAAGCAG ACTTCAGCTCCAGAGCCAGGGCTGCCTTCAGCTTTCCATCGGAGGTGCCCTCCAAGGCTAAAGTAGTGTG GGAGAAGTTCTCCAGGCAGCCCAAGTTTGAAAGAGACTTATTCTATCATGGCACATTCCGGGATGACTTC CTGTGGGGCGTATCCTCTTCAGCCTATCAAATCGAAGGAGGTTGGGATGCTGATGGCAAAGGCCCCAGCA TCTGGGATAACTTCACGCACACCCCAGGGAACGGTGTGAAAGACAATGCCACGGGAGACATAGCGTGTGA CAGCTATCATCAACTGGACGCTGACATAAACATCCTTCGAACTCTGAAGGTCAAGTCCTATCGATTTTCC ATCTCCTGGCCTCGAATTTTCCCAACGGGAAGAAACAGTTCCATCAACAAACAAGGTGTTGATTATTACA ACAAACTGATTGACAGATTGTTGGAGAGCAACATCTTCCCCATGGTGACACTCTTTCACTGGGACCTGCC CCAGGCCCTCCAGGATATTGGAGGTTGGGAGAACCCTTCTTTGATTGAGTTGTTCGATAGCTATGCGGAC TTCTGTTTTAAGACCTTTGGTGACAGAGTCAAGTTCTGGATGACCTTTAATGAGCCTTGGTGTTCTGCAG TGTTAGGCTACAGTTCAGGGATATTCCCCCCAAATGTGCAGGACCCGGGCTCACTATCTTACAAGGTTAG CCATGTAATCATTAAGGCACACGCCAGAGTCTACCATACTTATGATGAGAAGTACAGGCAGGAGCAGAAG GGGGTCATCTCTCTGAGCCTCAACACTCACTGGGTCGAACCCAAGGACCCGGGGGTCCAAAGGGACGTGG AAGCCGCTGACCGGATGCTGCAGTTTAACCTGGGCTGGTTTGCCCACCCTATTTTTAAAAACGGGGACTA TCCAGATGTCATGAAATGGAATGTGGGGAACAGGAGTGAGCTGCAGCACCTGGCCAGCTCCCGCCTGCCC AGCTTCACAGAAGAGGAGAAGAATTACATCAGAGGCACCGCCGATGTCTTCTGCATCAACACCTACACCT CTGTATTTGCACAGCATGTCACTCCTAGACTGAACCCACCCAGCTATGATAATGACATGGAGTTAAAGGC CAGTGACATGAACTCTTCAGCCTTGATCTCCATGATGCACCAAGACGTGCCCTGGGGGATGAGGAGGTTG CTGAACTGGATCAAGGAGGAGTATGGCAACATTCCCATTTACATCACTGAGAATGGACAGGGGCTGACAA ACCCCACGCTGGATGACACGGAAAGGATATTCTACCACAAAACCTATATTAATGAGGCTCTGAAAGCCTA CAGGCTGGATGGTGTTGACCTTCGAGGCTACTCTGCCTGGGCGCTGATGGACAACTTCGAGTGGCTGCAC GGCTACACCATGAGATTTGGATTGTATCACGTCGACTTTGATCATGTGAACAGACCTCGAACAGCAAGAG CCTCAGCCAGATACTATACAGAGGTCATTACCAACAATGGCATGCCCCTAGCCAAGGAGGATGAGTTCCT GTATGGAGAGTTTCCCAAGGGCTTCATCTGGAGTGCAGCGTCTGCTTCCTATCAGGTTGAAGGTGCGTGG AGAGCAGATGGCAAAGGACTTAGTATCTGGGACACATTTTCCCACACTCCACTGAAGATTGGGAATGACG ACAACGGGGACGTGGCATGTGACAGTTACCACAAGATTGCTGAGGATGTGGTGGCCCTTCAGAACCTGGG CGTGTCCCACTACCGCTTCTCCATCTCCTGGCCTAGAATCCTCCCCGACGGAACCACCAAGTTCATCAAC GAAGCAGGCCTCAACTACTATGTTCGATTCATAGATGCGCTGCTGGCTGCTGGCATCACACCACAGGTGA CCATGTATCACTGGGACCTGCCCCAGGCACTGCAGGACGTTGGGGGCTGGGAGAACGAGACAGTTGTACA GCGGTTTAAGGACTATGCCGATGTGCTCTTCCGGAGGTTGGGGGACAAGGTCAAGTTCTGGATCACACTG AATGAGCCCTTTGTCATTGCGGCCCACGGCTACGGCAGTGGGGTGTCTGCTCCAGGGATCTCTTTCAGAC CTGGCACAGCTCCGTACACTGCTGGACACAACCTAATAAAGGCTCACGCTGAGGCTTGGCATCTCTACAA CAGCACATACCGTAACAGCCAGGGAGGTGTGATCTCTATCACCATCAGCAGCGACTGGGCCGAGCCCAGA GATCCTTCCAACCAGGAGGATGTGGAGGCGGCCAGGAGATACGTACAGTTTATGGGGGGCTGGTTTGCAC ACCCCATCTTCAAAAACGGAGACTACCCTGAGGTGATGAAGACACGGATTCGTGACAGAAGCCTGGCGGC AGGCCTCAACAAGTCCAGGCTCCCTGAATTTACAGAGAGCGAGAAGAAGAAGATCCAGGGCACCTTTGAC TTTTTTGGGTTCAACCACTACACCACTGTCCTAGCCTACAACCTCAACTACGCCGCTGCTGTTTCGTCTT TTGATGCCGATAGGGGAGTTGCTTCCATTACAGACCGCTCCTGGCCAGACTCTGGCTCCTTCTGGCTGAA GATGACCCCTTTTGGCTTCCGGAGGATCTTGAACTGGTTGAAGGAGGAGTACAACAACCCTCTAATTTAT GTCACAGAGAATGGAGTGTCCCGACGAGGAGACCCAGAACTCAATGACACCGACAGGATCTACTACCTCC GCAGCTACATTAATGAAGCCCTCAAAGCTGTACGAGATAAGGTGGACCTTCGAGGGTACACGGTCTGGAG CATCATGGACAACTTTGAATGGGCCACAGGCTTCGCAGAGAGGTTCGGCGTGCACTTTGTGAACCGCTCT GACCCTTCTTTGCCAAGGATCCCCAAGGCGTCAGCCAAGGTCTACGCCTCCATAGTCCGCTGCAATGGCT TTCCTGACCCTGCACAAGGACCTCATCCTTGTCTCCAGCAACCAGAAGATGCTGGGCCCACTGCCAGCCC TGTGAAGTCAGAGGTGCCCTTCCTGGGGCTGATGCTGGGCACGGCAGAAGCACAGACGGCTCTGTATGTC CTCTTCGCTCTTGTGCTGCTTGGAGTTTGCAGCGTGGCATTTCTGTTATACAAATACTGCAAGCGCTCCA AGCAAGGGACAACACAGCCAGGCCACCACGGTCTGAGTCAAATTTCTTCCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225984 representing NM_001081078 Red=Cloning site Green=Tags(s) MELPWTALFLSTFLLGLSCQGNDWESDRNFISAAGPLTNDLVHNLNHPLGKQGSDLVAGDTDIYVCQQPL PSFLPQYFSSLRASQVTHYKVLLSWAQLLPKGSSKNPDQEAVQCYRQLLQSLKDAQLQPMVVLFHQMPPT STIQRDGGFADLFADYATLAFQSFGDLVEIWFTFSDLEKVIMGLPHQHLKASGLQTLSDAHRKAFDVYHR KYSSQGGKLSVVLKAEDLPKLLPDPSSAALVKGSVDFLSLDLSYDCQSISTLPQKLSELQNLEPKVKVFV YTLKPQDCPSTGMSPASLLFRLLEAINKDQVQTVGFDVSAFLSCTSSSEESPSCSLTYSSLALQSEQQHE TVVSSFPGSAYQRVWAALANQSREERDAFLQDVFPEGFLWGVSTGAFNVEGGWAEGGRGPSIWDHYSNLN AAESQATAKVASDSYHKPVSDVALLRGLRADVYKFSISWSRIFPFGQRTSPNLQGVAYYNKLIDSLLDSQ VEPMATLFHWDLPQALQEQGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVISYAGYGTGQHAP AISDPGVASFKVAHLILKAHARTWHHYNYHHRQKQQGRVGIVLNSDWAEPLDGKSPQDLAAAERYLHFML GWFAHPIFIDGDYPAALRAQIQHTNQQCGRPLAQLPEFTEAEKRLLKGSADFLGLSHYTSRLISKAGQQT CIPSYDNIGGFSQHVDPKWPQTASPWIRVVPWGIRRLLRFASLEYTKGKLPIFLAGNGMPIGEGSDLFDD SMRVNYLNLYINEVLKAVKEDSVDVRSYIARSLIDGYEGPLGYSQRFGLYHVNFNDSSRPRTPRKSAYFF TSIIEKNGFPAKKVKRNPLPLKADFSSRARAAFSFPSEVPSKAKVVWEKFSRQPKFERDLFYHGTFRDDF LWGVSSSAYQIEGGWDADGKGPSIWDNFTHTPGNGVKDNATGDIACDSYHQLDADINILRTLKVKSYRFS ISWPRIFPTGRNSSINKQGVDYYNKLIDRLLESNIFPMVTLFHWDLPQALQDIGGWENPSLIELFDSYAD FCFKTFGDRVKFWMTFNEPWCSAVLGYSSGIFPPNVQDPGSLSYKVSHVIIKAHARVYHTYDEKYRQEQK GVISLSLNTHWVEPKDPGVQRDVEAADRMLQFNLGWFAHPIFKNGDYPDVMKWNVGNRSELQHLASSRLP SFTEEEKNYIRGTADVFCINTYTSVFAQHVTPRLNPPSYDNDMELKASDMNSSALISMMHQDVPWGMRRL LNWIKEEYGNIPIYITENGQGLTNPTLDDTERIFYHKTYINEALKAYRLDGVDLRGYSAWALMDNFEWLH GYTMRFGLYHVDFDHVNRPRTARASARYYTEVITNNGMPLAKEDEFLYGEFPKGFIWSAASASYQVEGAW RADGKGLSIWDTFSHTPLKIGNDDNGDVACDSYHKIAEDVVALQNLGVSHYRFSISWPRILPDGTTKFIN EAGLNYYVRFIDALLAAGITPQVTMYHWDLPQALQDVGGWENETVVQRFKDYADVLFRRLGDKVKFWITL NEPFVIAAHGYGSGVSAPGISFRPGTAPYTAGHNLIKAHAEAWHLYNSTYRNSQGGVISITISSDWAEPR DPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYPEVMKTRIRDRSLAAGLNKSRLPEFTESEKKKIQGTFD FFGFNHYTTVLAYNLNYAAAVSSFDADRGVASITDRSWPDSGSFWLKMTPFGFRRILNWLKEEYNNPLIY VTENGVSRRGDPELNDTDRIYYLRSYINEALKAVRDKVDLRGYTVWSIMDNFEWATGFAERFGVHFVNRS DPSLPRIPKASAKVYASIVRCNGFPDPAQGPHPCLQQPEDAGPTASPVKSEVPFLGLMLGTAEAQTALYV LFALVLLGVCSVAFLLYKYCKRSKQGTTQPGHHGLSQISSF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081078
ORF Size	5793 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081078.2, NP_001074547.1
RefSeq Size	6166 bp
RefSeq ORF	5796 bp
Locus ID	226413
MW	218.3 kDa

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