Numb (NM_010949) Mouse Tagged ORF Clone
CAT#: MR225927
- TrueORF®
Numb (Myc-DDK-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 2
ORF Plasmid: tGFP
"NM_010949" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,496.00
CNY 5,320.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | m-num; Nb |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225927 representing NM_010949
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAACTACGGCAAAGCTTCAGGAGAAAGAAAGACGTTTATGTCCCAGAGGCCAGCCGTCCACATC AGTGGCAGACAGATGAAGAAGGAGTCCGCACTGGAAAGTGTAGCTTCCCAGTTAAGTACCTCGGCCACGT AGAAGTTGATGAGTCAAGAGGAATGCACATCTGTGAAGATGCCGTAAAGAGATTGAAAGCTGAAAGGAAG TTCTTCAAAGGCTTCTTTGGAAAAACGGGAAAGAAAGCAGTGAAGGCCGTTCTGTGGGTGTCAGCGGATG GGCTCAGAGTTGTGGACGAGAAAACTAAGGACCTCATAGTTGACCAGACAATAGAAAAAGTTTCCTTCTG TGCCCCAGATAGGAACTTTGACAGAGCCTTTTCTTACATATGTCGTGATGGCACCACTCGGCGATGGATC TGTCATTGTTTCATGGCTGTCAAAGACACGGGGGAAAGACTGAGCCACGCCGTGGGCTGTGCTTTTGCAG CCTGTTTAGAGCGTAAACAGAAGCGGGAGAAGGAGTGTGGCGTCACTGCTACTTTTGATGCCAGTAGAAC CACTTTCACAAGAGAAGGATCATTCCGTGTCACAACTGCCACTGAGCAAGCCGAAAGAGAGGAGATCATG AAACAGTTGCAAGATGCCAAGAAAGCTGAGACAGACAAGACAGTTGTTGGTCCATCAGTGGCTCCTGGCA ACACTGCTCCATCCCCATCCTCTCCCACCTCTCCCACTCCGGATGGCACTGCATCTTCAGAGATGAACAA TCCCCATGCTATCCCACGCCGGCATGCACCAATTGAACAGCTTGCTCGTCAAGGCTCTTTCCGGGGATTT CCTGCTCTTAGCCAGAAGATGTCACCCTTTAAACGCCAGCTGTCCCTACGCATCAATGAGTTGCCTTCCA CTATGCAGAGGAAGACCGATTTCCCAATAAAAAACACAGTGCCCGAGGTGGAAGGAGAGGCCGAGAGCAT CAGCTCCTTGTGTTCCCAGATCACCAGTGCCTTCAGCACGCCCTCTGAGGACCCCTTCTCCTCCGCCCCA ATGACCAAACCAGTGACATTGGTGGCACCACAGTCTCCTGTGTTACAAGGGACTGAGTGGGGTCAGTCTT CTGGTGCTGCCTCTCCAGGTCTCTTCCAGGCTGGTCACAGACGCACTCCCTCTGAAGCTGACCGCTGGTT AGAAGAAGTGTCAAAGAGTGTGCGGGCCCAGCAGCCTCAGGTCTCAGCTGCCCCTCTGCAGCCAGTTCTC CAGCCGCCTCCGCCCGCCGCCATTGCCCCTCCAGCACCTCCTTTCCAAGGACATGCCTTCCTCACGTCCC AGCCTGTGCCCGTGGGTGTGGTCCCACCCCTACAACCAGCCTTTGTCCCTACCCAGTCCTACCCTGTGGC CAACGGGATGCCCTACCCAGCCTCTAATGTGCCTGTAGTGGGCATCACCCCATCCCAGATGGTAGCCAAT GTGTTTGGCACTGCAGGCCACCCTCAGACAACTCATCCACATCAGTCGCCAAGCCTGGCCAAGCAGCAGA CATTCCCTCAATATGAGACAAGTAGTGCTACCACCAGTCCCTTCTTTAAGCCTCCTGCTCAGCACCTCAA TGGTTCTGCAGCTTTCAATGGTGTAGACAATGGTGGGCTAGCCTCAGGAAACAGGCATGCAGAAGTCCCT CCAGGCACCTGCCCAGTGGATCCTTTCGAAGCTCAGTGGGCCGCACTAGAAAGCAAGTCCAAGCAGCGTA CAAATCCTTCTCCTACCAACCCTTTCTCCAGTGACTTACAGAAAACATTTGAAATAGAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225927 representing NM_010949
Red=Cloning site Green=Tags(s) MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERK FFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWI CHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIM KQLQDAKKAETDKTVVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGF PALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAP MTKPVTLVAPQSPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVL QPPPPAAIAPPAPPFQGHAFLTSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVAN VFGTAGHPQTTHPHQSPSLAKQQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVP PGTCPVDPFEAQWAALESKSKQRTNPSPTNPFSSDLQKTFEIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_010949 |
ORF Size | 1812 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010949.2, NP_035079.1 |
RefSeq Size | 1815 bp |
RefSeq ORF | 1815 bp |
Locus ID | 18222 |
UniProt ID | Q9QZS3 |
MW | 66.2 kDa |
Gene Summary | This gene encodes a conserved protein that is distributed asymmetrically during cell division in the developing embryo. The encoded protein participates in cell fate decisions by interacting with the Notch receptor. Loss of function of this gene results in severe defects in neural development and loss of viability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219614 | Numb (untagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 2, (10ug) |
CNY 7,790.00 |
|
MG225927 | Numb (tGFP-tagged) - Mouse numb gene homolog (Drosophila) (Numb) transcript variant 2, (10ug) |
CNY 5,890.00 |
|
MR225927L3 | Lenti ORF clone of Numb (Myc-DDK-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 2 |
CNY 7,220.00 |
|
MR225927L4 | Lenti ORF clone of Numb (mGFP-tagged) - Mouse numb gene homolog (Drosophila) (Numb), transcript variant 2 |
CNY 7,220.00 |