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Cdk6 (NM_009873) Mouse Tagged ORF Clone
CAT＃: MR225890
- TrueORF®
Cdk6 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 6 (Cdk6)
ORF Plasmid: tGFP
"NM_009873" in other vectors (4)
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CNY 3,600.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI504062; Crk; Crk2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225890 representing NM_009873
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGGACAGCCTGAGTCGCGCCGATCAGCAGTATGAGTGCGTGGCGGAGATCGGCGAAGGCGCCT ATGGGAAGGTGTTCAAGGCCCGCGACCTGAAGAACGGCGGCCGCTTCGTGGCTCTGAAGCGCGTGCGAGT GCAGACCAGTGAGGAGGGCATGCCGCTCTCCACCATCCGCGAGGTGGCGGTGCTGAGGCACCTGGAGACC TTCGAGCACCCCAACGTGGTCAGGTTGTTTGATGTGTGCACAGTGTCACGGACGGACAGAGAAACCAAGC TTACACTAGTGTTTGAGCATGTTGATCAAGACTTGACCACTTACTTGGATAAAGTTCCAGAGCCCGGCGT ACCCACAGAAACCATAAAGGATATGATGTTTCAGCTTCTCCGAGGTCTGGACTTTCTTCATTCTCACAGA GTAGTGCATCGTGATCTGAAACCGCAGAACATTCTGGTGACCAGCAGTGGACAGATAAAGCTGGCTGACT TTGGCCTTGCCCGCATCTATAGTTTTCAGATGGCCCTTACCTCGGTGGTCGTCACGCTGTGGTACCGAGC CCCAGAAGTCCTGCTCCAGTCCAGCTATGCCACCCCTGTGGACCTCTGGAGTGTCGGTTGCATCTTTGCA GAAATGTTTCGCAGAAAGCCTCTTTTTCGTGGAAGTTCAGACGTGGATCAACTAGGAAAAATCTTGGACA TCATTGGACTCCCAGGAGAGGAAGACTGGCCTAGGGACGTGGCCCTTCCCCGGCAGGCTTTTCATTCCAA ATCTGCTCAACCCATCGAGAAGTTTGTGACAGATATTGACGAACTAGGCAAAGACCTACTTCTGAAATGC CTGACGTTTAATCCAGCTAAAAGGATATCCGCCTACGGCGCCCTGAATCACCCGTACTTCCAAGATCTGG AGAGATACAAGGACAACCTGAACTCTCACCTGCCATCCAACCAGAGCACCTCGGAGCTGAACACAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225890 representing NM_009873
Red=Cloning site Green=Tags(s) MEKDSLSRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTSEEGMPLSTIREVAVLRHLET FEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHR VVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFA EMFRRKPLFRGSSDVDQLGKILDIIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKC LTFNPAKRISAYGALNHPYFQDLERYKDNLNSHLPSNQSTSELNTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009873 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009873.3, NP_034003.1 |
| RefSeq Size | 2470 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 12571 |
| UniProt ID | Q64261 |
| MW | 37.5 kDa |
| Gene Summary | This gene encodes a member of the cyclin dependent kinase family of proteins that play important roles in the progression and regulation of the cell cycle. The encoded protein binds to a D-type cyclin to form an active kinase complex to regulate progression through the G1 phase of the cell cycle. Mice lacking the encoded protein exhibit thymic and splenic hypoplasia, and hematopoietic defects such as reduced number of megakaryocytes and erythrocytes. A pseudogene of this gene has been defined on chromosome 4. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208271 | Cdk6 (untagged) - Mouse cyclin-dependent kinase 6 (Cdk6), (10ug) |
CNY 3,600.00 |
|
| MG225890 | Cdk6 (tGFP-tagged) - Mouse cyclin-dependent kinase 6 (Cdk6), (10ug) |
CNY 4,370.00 |
|
| MR225890L3 | Lenti ORF clone of Cdk6 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 6 (Cdk6) |
CNY 5,890.00 |
|
| MR225890L4 | Lenti ORF clone of Cdk6 (mGFP-tagged) - Mouse cyclin-dependent kinase 6 (Cdk6) |
CNY 5,890.00 |
