United States
Germany
Japan
United Kingdom
China
Aloxe3 (NM_011786) Mouse Tagged ORF Clone
CAT＃: MR225887
- TrueORF®
Aloxe3 (Myc-DDK-tagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3)
ORF Plasmid: tGFP
"NM_011786" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,216.00
CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | e-LOX-3; eLOX-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225887 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTATATCGGCTGTGTGTGACCACTGGTTCCTACCTGAAGGCTGGCACATTGGATAACATCTATG CTACATTGGTAGGCACCTGTGGTGAAAGCCCTAAGCAGAAGCTGGATCGCGTAGGCAGGGACTTCGCCTC TGGATCGGTTCAGAAGTACAAGGTGCGCTGTGAAGCAGAGCTGGGTGAGATCTTGCTGTTGCGCTTACAC AAGGAGCGCTTTGCTTTCTTCTGCAAGGACCCTTGGTACTGCAGTCGCATCTGTGTCACTGCCCCCGATG GCTCTGCTGTCCACTTTCCCTGCTACCAGTGGATCGATGGCTACTGTACTGTGGAGCTGCGGCCAGGAAC AGCAAGAACCATCTGTCAGGATTCTCTTCCCCTCCTTCTGGACCACAGGAAACGGGAACTCCGAGCCCGC CAAGAATGTTATCGCTGGAAGATCTTTGCCCCTGGCTTCCCTCGGATGGTGGATGTCAGCAGCTTTCAGG AGATGGAGTCAGATAAGAAATTTGCCTTGACCAAGACAGTACCTTGTGCAGAACAGGATGACAACTCTGG GAACCGGTACCTGCCTGGCTTTCCTATGAAGATTGACATCCCATCTCTGCTGCACATGGAACCCAACATT CGATACTCAGCCACCAAGACGGCCTCACTGATCTTCAACGCCCTTCCTGCATCCTTTGGCATGAAGATTC GAGGTCTCCTGGACCGCAAGGGCTCCTGGAAGAGGCTGGACGACATCCGGAACATCTTCTGGTGCCATAA GACCTTCACTTCAGAGTACGTCACCGAGCATTGGTGTGAGGACAGCTTCTTTGGGTACCAGTACCTGAAT GGTGTCAACCCTGTCATGCTTCATTGCCTCTCCAGCTTGCCCAGCAAGCTGCCTGTCACCAATGACATGG TGGCACCTTTGCTGGGACCAGGCACCTGCCTACAAACAGAGCTAGAGAGGGGGCACATCTTCCTGGCGGA CTACTGGATCCTGGCGGAGGCCCCAGTCCATTGCATAAACAGTCTCCAACAGTACGTAACAGCGCCCCTC TGCCTGTTGTGGCTCAACCCACAGGGGGTGCTGCTGCCATTGGCAATCCAGCTCAGCCAGACACCAGGGC CAGAGAGCCCCATCTTTCTGCCCACTGATTGCGAGTTGGACTGGCTGCTGGCCAAGACGTGGGTGCGCAA CTCTGAGTTTTTGGTGCACGAGAACAACACGCATTTTCTGTGCACGCATTTGCTATGCGAGGCCTTCTCC ATGGCTACACTGCGTCAGCTGCCGCTCTGTCATCCAGTCTACAAGCTCCTGCTTCCTCATACTCGCTACA CGCTGCAAGTGAACACCATCGCAAGAGCCACGCTGCTCAACCCAGACGGCCTCGTGGACAAGGTCACGTC CATCGGTAGGCAGGGCCTCATCTACCTCATGAGCACCGGGCTGGCCCACTTCACCTACACGGATTTCTGC CTACCGGATAGCATACGGGCTCGTGGCGTCCTGACCATTCCCAACTACCACTACCGAGACGACGGTCTGA AGATCTGGGCGGCTATTGAGAGGTTTGTCTCAGAGATTGTGAGTTACTATTATCCCAGCGATGCGTCTGT GCAGCAGGACTGTGAACTGCAGGCCTGGGTGGGTGAGATTTTTGCTCAGGCGTTCCTCGGACGGGAAAGT TCAGGCTTCCCAAGCCGGCTGTGCACCCCAGGAGAGCTCGTGAAGTATCTCACCGCAATCATCTTTAATT GCTCCGCCCAGCACGCTGCGGTCAATAGTGGGCAGCATGACTTTGGGGCCTGGATGCCCAACGCCCCATC ATCCATGAGGCAGCCTCCGCCTCAGACCAAGGGCGACACGACAATGAAGAGTTACCTAGACACCCTCCCA GAGGTGAACACCACCTGTAGAAACCTTCTCCTCTTCTGGCTGGTCAGCCAAGAGCCAAAGGACCAGAGGC CTCTGGGCACCTACCCAGATGAACACTTCACAGAGGAGGCCCCACGGCAGAGCATCGCAGCCTTCCAGAA CTGCCTGGCCCAGATCTCAAAGGACATCAGGGAGCGCAACCAGAGCCTGGCACTGCCCTATGCCTACTTG GATCCTCCACTCATTGAGAACAGTGTTTCCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225887 protein sequence
Red=Cloning site Green=Tags(s) MAVYRLCVTTGSYLKAGTLDNIYATLVGTCGESPKQKLDRVGRDFASGSVQKYKVRCEAELGEILLLRLH KERFAFFCKDPWYCSRICVTAPDGSAVHFPCYQWIDGYCTVELRPGTARTICQDSLPLLLDHRKRELRAR QECYRWKIFAPGFPRMVDVSSFQEMESDKKFALTKTVPCAEQDDNSGNRYLPGFPMKIDIPSLLHMEPNI RYSATKTASLIFNALPASFGMKIRGLLDRKGSWKRLDDIRNIFWCHKTFTSEYVTEHWCEDSFFGYQYLN GVNPVMLHCLSSLPSKLPVTNDMVAPLLGPGTCLQTELERGHIFLADYWILAEAPVHCINSLQQYVTAPL CLLWLNPQGVLLPLAIQLSQTPGPESPIFLPTDCELDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFS MATLRQLPLCHPVYKLLLPHTRYTLQVNTIARATLLNPDGLVDKVTSIGRQGLIYLMSTGLAHFTYTDFC LPDSIRARGVLTIPNYHYRDDGLKIWAAIERFVSEIVSYYYPSDASVQQDCELQAWVGEIFAQAFLGRES SGFPSRLCTPGELVKYLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGDTTMKSYLDTLP EVNTTCRNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRQSIAAFQNCLAQISKDIRERNQSLALPYAYL DPPLIENSVSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011786 |
| ORF Size | 2136 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011786.1, NM_011786.2, NP_035916.2 |
| RefSeq Size | 2538 bp |
| RefSeq ORF | 2136 bp |
| Locus ID | 23801 |
| UniProt ID | Q9WV07 |
| MW | 80.5 kDa |
| Gene Summary | Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220771 | Aloxe3 (untagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3), (10ug) |
CNY 9,120.00 |
|
| MG225887 | Aloxe3 (tGFP-tagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3), (10ug) |
CNY 6,940.00 |
|
| MR225887L3 | Lenti ORF clone of Aloxe3 (Myc-DDK-tagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3) |
CNY 8,170.00 |
|
| MR225887L4 | Lenti ORF clone of Aloxe3 (mGFP-tagged) - Mouse arachidonate lipoxygenase 3 (Aloxe3) |
CNY 8,170.00 |
