Spast (NM_001162870) Mouse Tagged ORF Clone
CAT#: MR225751
- TrueORF®
Spast (Myc-DDK-tagged) - Mouse spastin (Spast), transcript variant 1
ORF Plasmid: tGFP
"NM_001162870" in other vectors (4)
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CNY 6,856.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | mKIAA1083; Spg4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225751 representing NM_001162870
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCTCCGGCCGGACGACGGAAGAAGAAAGGCTCGGGCGGCGCGAGCCCGGCGCCCGCCAGGCCTC CGCCCCCCGCCGCGGTCCCCGCCCCTGCCGCCGGCCCGGCCCCTGCGGCCGGCTCGCCGCCTAAGCGGAA CCCGTCTTCTTTCTCGTCCCCGCTGGTCGTCGGCTTCGCCCTGCTGCGCCTGCTGGCCTGCCACCTGGGG CTCCTCTTCGCGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCCGCCAAGAGGAGCTCCGGGACCG CGCCGGCGCCCGCCTCGCCCTCGCCCCCAGAGCCCGGACCGGGTGGCGAGGCCGAGAGCGTCCGCGTCTT CCACAAGCAGGCCTTCGAGTACATCTCCATTGCCCTGCGCATCGACGAGGAAGAGAAAGCAGGACAGAAG GAACAAGCTGTGGAATGGTATAAGAAAGGTATCGAAGAACTGGAAAAAGGAATCGCTGTTATAGTTACGG GCCAAGGTGAACAGTATGAAAGAGCTAGACGTCTTCAAGCCAAAATGATGACTAATTTAGTTATGGCCAA GGACCGTTTACAACTTCTAGAGAAGCTGCAACCAGTTTTGCAATTTTCCAAGTCACAGACGGACGTCTAT AACGAGAGTACTAACCTGACATGCCGCAATGGACATCTCCAGTCAGAAAGTGGAGCAGTTCCGAAGAGGA AAGACCCCTTAACACATGCTAGTAATTCATTGCCTCGATCAAAAACTGTCCTGAAAAGTGGCTCCGCAGG GCTCTCCGGTCACCACAGGGCGCCTAGTTGCAGTGGTTTGTCCATGGTTTCTGGAGCAAGACCGGGACCT GGTCCTGCAGCTACCACACATAAGGGTACTCCAAAACCAAATAGAACCAACAAACCTTCTACTCCCACAA CTGCAGTTCGGAAAAAGAAAGACTTGAAAAATTTTAGGAATGTGGACAGCAATCTTGCTAACCTTATAAT GAATGAAATTGTTGACAATGGGACAGCTGTTAAGTTTGATGACATAGCCGGGCAGGAGCTGGCAAAGCAA GCGCTGCAGGAGATTGTCATCCTTCCTTCTCTGCGGCCTGAGTTGTTCACAGGGCTCAGAGCTCCTGCTA GAGGCTTGTTACTCTTCGGTCCGCCAGGAAACGGAAAAACAATGCTGGCTAAAGCAGTAGCTGCAGAGTC TAATGCGACCTTTTTCAACATAAGTGCTGCCAGTTTAACTTCAAAATATGTGGGAGAAGGAGAGAAATTG GTGAGAGCTCTCTTTGCTGTGGCTCGAGAACTTCAACCATCTATAATTTTTATAGATGAAGTTGACAGTC TTTTGTGTGAGAGACGGGAAGGGGAGCACGACGCTAGCAGACGGCTAAAGACGGAATTTTTAATAGAATT TGACGGGGTGCAATCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAACAGGCCCCAAGAGCTT GATGAAGCTGTTCTCAGGCGTTTCATTAAACGGGTATATGTGTCCTTACCAAATGAGGAGACAAGACTCC TTCTGCTTAAAAACCTGTTGTGTAAACAAGGAAGTCCACTGACCCAAAAAGAACTCGCACAGCTTGCTAG AATGACCGATGGATACTCTGGAAGTGATCTGACCGCTTTGGCCAAGGATGCAGCCCTGGGTCCTATCCGA GAACTGAAGCCAGAGCAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCA CAGAATCCTTAAAAAAGATAAAACGCAGTGTGAGTCCTCAGACCTTAGAAGCATACATACGCTGGAACAA GGATTTTGGAGACACCACTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225751 representing NM_001162870
Red=Cloning site Green=Tags(s) MSSPAGRRKKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLG LLFAWLCQRFSRALMAAKRSSGTAPAPASPSPPEPGPGGEAESVRVFHKQAFEYISIALRIDEEEKAGQK EQAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFSKSQTDVY NESTNLTCRNGHLQSESGAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGP GPAATTHKGTPKPNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQ ALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKL VRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQEL DEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIR ELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001162870 |
ORF Size | 1842 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001162870.1, NP_001156342.1 |
RefSeq Size | 4696 bp |
RefSeq ORF | 1845 bp |
Locus ID | 50850 |
UniProt ID | Q9QYY8 |
MW | 66.9 kDa |
Gene Summary | ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:19141076 PubMed:20530212). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (By similarity). Severing activity is not dependent on tubulin acetylation or detyrosination (By similarity). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation (By similarity). It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia (By similarity). SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (By similarity). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (By similarity). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (By similarity). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (By similarity). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (By similarity). Probably plays a role in axon growth and the formation of axonal branches (PubMed:18234839).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219723 | Spast (untagged) - Mouse spastin (Spast), transcript variant 1, (10ug) |
CNY 7,890.00 |
|
MG225751 | Spast (tGFP-tagged) - Mouse spastin (Spast) transcript variant 1, (10ug) |
CNY 6,650.00 |
|
MR225751L3 | Lenti ORF clone of Spast (Myc-DDK-tagged) - Mouse spastin (Spast), transcript variant 1 |
CNY 7,980.00 |
|
MR225751L4 | Lenti ORF clone of Spast (mGFP-tagged) - Mouse spastin (Spast), transcript variant 1 |
CNY 7,980.00 |