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Dmrt2 (NM_145831) Mouse Tagged ORF Clone
CAT＃: MR225591
- TrueORF®
Dmrt2 (Myc-DDK-tagged) - Mouse doublesex and mab-3 related transcription factor 2 (Dmrt2)
ORF Plasmid: tGFP
"NM_145831" in other vectors (4)
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CNY 6,264.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Terra |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225591 representing NM_145831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAAGGGCAGGCAGTCCCGGGAGTCGGGGACTGGGAGATCGACGTGGAAAGCCTAGACCTGGAGG AAGATAGCTGTGGGACTCCACTGCGGGCCACGCCGCCGCAGGAACCCAGTCCGGCGGCGGCCGATGGGGA GGAGGACGAGGACGAAGAAGAGGAGGATGAAGATGTGGAGGACGAGGGTGACGGCGAGGAGCCCGGCGTG TCCTCGGAGGTGCCAGGCCGGCCAGAGCAGCCCGGGGGACTGGCACCCAGGCCACCGCCTGCTGCTCAGG CGCTGCCAGCCGCCGCCGCCGCCCCTGAGCGCGGTGCCACCGCCGGTGGCGGCGCAGAGCCGCGCAAACT GAGCCGCACACCCAAGTGCGCACGCTGCCGCAACCACGGCGTGGTATCCTGTCTCAAGGGCCACAAGCGA TTCTGTCGCTGGCGGGACTGCCAGTGCGCCAACTGCCTGCTGGTGGTGGAGCGCCAGCGTGTCATGGCCG CCCAGGTGGCGCTCCGGAGGCAGCAGGCCACAGAGGACAAGAAGGGGCTTTCTGGGAAACAGAACAATTT CGATCGGAAAGCAGTGTACCAGAGGCAAGTCAGGGCGCCGAGTTTGCTGGCCAAAAGCATTTTAGAAGGT TATCGCCCAATGACGGCAGAGACTTACCTAGGAGGGACCCTTCCTCTACCTCCGCCTGTCAGTGACAGGA TGAGGAAGAGAAGAGCCTTTGCTGACAAGGAGTTGGAGAACATTATGCTGGAGAGAGAATATAAAGAGAG GGAGATGTTAGAAACATCTCAAGCTGCTGCCTTATTCCTGCCCAACCGTATGGTGCCTGGACCCGAGTAC AGTTCCTACAAAGGTACCTACAGCCCCACTGCAGGAGAGCTGCCGAGCAAGGACTTCTGTAACTTTTTAC CTACCTGCCTTGATCTCACCATGCAGTATTCAGGGTCTGGGAATATGGAACTTATTTCTTCTAACGTCAG TGTGGCCACAACTTACAGACAATATCCCCTGTCCTCACGATTTTTAGTTTGGCCCAAGTGCGGTCCCATT AGTGACACCCTTCTCTACCAGCAATATCTGTTAAATGCTACCACTTCTGTCCAAGCTCTGAAGCCGGGGA CCGGCTGGGACTTGAAAGGAACCCGAGTTCAGGATGGGCTTAGCGCAGAGCAAGACATGATGCCACCCAA ACTGGAAGGCTCTCTGGTGCTGCCCCATCTGCCAGAGGTCCCAGCCTCGAGAACAGACCTTCAGGTTCAC CAGGTGGTTCCAGAGAGGTCTGCCTTCTCCCCACCCGGTCGGAATTTCTCTCCCATTGTTGATATGGACT GCCTGGCAGCTCAAGGGCACGTCTTAACCAAGCTCAGCAAAGAAAACACTAGACCTTCTCTGCCACTTAA AACTAATCCATTCCACTCAGTATTCCAGCAGACGCTCAGCGACAAATCAGGCCCTGAGTTGAACGCACCA TTTGTCAAAGAAGCCTTTGAAGAGACCCCAAAGAAACACAGAGAGTGTTTGGTCAAGGAGAGCCAGAAGT ACACATTTACAATAGACAGATGCGCAAAAGACCTCTTTGTAGCCAAACAAGTTGGAACGAAACTTTCGGC GAATGAGCCACTGTCGTTCTCTGTCGAATCTATTCTTAAGAGGCCTTCATCTGCCGTCACTCACGTCTCT CAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225591 representing NM_145831
Red=Cloning site Green=Tags(s) MTEGQAVPGVGDWEIDVESLDLEEDSCGTPLRATPPQEPSPAAADGEEDEDEEEEDEDVEDEGDGEEPGV SSEVPGRPEQPGGLAPRPPPAAQALPAAAAAPERGATAGGGAEPRKLSRTPKCARCRNHGVVSCLKGHKR FCRWRDCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFDRKAVYQRQVRAPSLLAKSILEG YRPMTAETYLGGTLPLPPPVSDRMRKRRAFADKELENIMLEREYKEREMLETSQAAALFLPNRMVPGPEY SSYKGTYSPTAGELPSKDFCNFLPTCLDLTMQYSGSGNMELISSNVSVATTYRQYPLSSRFLVWPKCGPI SDTLLYQQYLLNATTSVQALKPGTGWDLKGTRVQDGLSAEQDMMPPKLEGSLVLPHLPEVPASRTDLQVH QVVPERSAFSPPGRNFSPIVDMDCLAAQGHVLTKLSKENTRPSLPLKTNPFHSVFQQTLSDKSGPELNAP FVKEAFEETPKKHRECLVKESQKYTFTIDRCAKDLFVAKQVGTKLSANEPLSFSVESILKRPSSAVTHVS Q TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145831 |
| ORF Size | 1683 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145831.3, NP_665830.1 |
| RefSeq Size | 2337 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 226049 |
| UniProt ID | Q8BG36 |
| MW | 62.1 kDa |
| Gene Summary | Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome. Is not required for sex determination and/or differentiation in embryonic development. Also not involved in symmetric somite formation and hence does not regulate the laterality pathway that controls left-right asymmetric organ positioning.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219010 | Dmrt2 (untagged) - Mouse doublesex and mab-3 related transcription factor 2 (Dmrt2), (10ug) |
CNY 6,696.00 |
|
| MG225591 | Dmrt2 (tGFP-tagged) - Mouse doublesex and mab-3 related transcription factor 2 (Dmrt2), (10ug) |
CNY 6,180.00 |
|
| MR225591L3 | Lenti ORF clone of Dmrt2 (Myc-DDK-tagged) - Mouse doublesex and mab-3 related transcription factor 2 (Dmrt2) |
CNY 7,510.00 |
|
| MR225591L4 | Lenti ORF clone of Dmrt2 (mGFP-tagged) - Mouse doublesex and mab-3 related transcription factor 2 (Dmrt2) |
CNY 7,510.00 |
