United States
Germany
Japan
United Kingdom
China
Crbn (NM_175357) Mouse Tagged Lenti ORF Clone
CAT＃: MR225531L3
- LentiORF®
Lenti ORF clone of Crbn (Myc-DDK-tagged) - Mouse cereblon (Crbn), transcript variant 2
"NM_175357" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR225531).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175357 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175357.2 |
| RefSeq Size | 4054 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 58799 |
| UniProt ID | Q8C7D2 |
| Gene Summary | This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215992 | Crbn (untagged) - Mouse cereblon (Crbn), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
| MG225531 | Crbn (tGFP-tagged) - Mouse cereblon (Crbn) transcript variant 2, (10ug) |
CNY 4,940.00 |
|
| MR225531 | Crbn (Myc-DDK-tagged) - Mouse cereblon (Crbn), transcript variant 2 |
CNY 4,024.00 |
|
| MR225531L4 | Lenti ORF clone of Crbn (mGFP-tagged) - Mouse cereblon (Crbn), transcript variant 2 |
CNY 6,370.00 |
