United States
Germany
Japan
United Kingdom
China
Crbn (NM_175357) Mouse Untagged Clone
CAT＃: MC215992
Crbn (untagged) - Mouse cereblon (Crbn), transcript variant 2, (10ug)
CNY 3,656.00
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215992 representing NM_175357
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCGAGGGAGATCAGCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCTGCCTGCAG ACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAAGATAGTAAAGAAGCCAGAAAACC GAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAGGAGTTC CACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATCCCAGTCCTTCCTGAGGTGCTGATGATCC TGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCACAGGAAGTCAGCATGGTGCGGAACTTAAT CCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTGGGACA ACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGCAATTG GAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAGAT TTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCAGTTAGAATCACTCAATAAGTGCCAGGTA TTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAAGAGAA AGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACATTAAT GGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGATGATTCTCTTCCTGAAAATCCA ATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCTTAAAA TCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCATGAACAAATGTACTTCCCTTTGCTGTAA ACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATTTAGTTTATCCTTATGTGGTCCAATGGCA GCATATGTGAATCCTCATGGATATGTACATGAGACACTGACTGTGTATAAAGCGTCCAACCTGAATCTGA TAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGCAAGATCTG TGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTA ACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTT GTTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_175357 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175357.2, NP_780566.1 |
| RefSeq Size | 4054 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 58799 |
| UniProt ID | Q8C7D2 |
| Gene Summary | This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the coding region compared to variant 1. It encodes isoform 2 which is longer than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225531 | Crbn (tGFP-tagged) - Mouse cereblon (Crbn) transcript variant 2, (10ug) |
CNY 4,940.00 |
|
| MR225531 | Crbn (Myc-DDK-tagged) - Mouse cereblon (Crbn), transcript variant 2 |
CNY 4,024.00 |
|
| MR225531L3 | Lenti ORF clone of Crbn (Myc-DDK-tagged) - Mouse cereblon (Crbn), transcript variant 2 |
CNY 6,424.00 |
|
| MR225531L4 | Lenti ORF clone of Crbn (mGFP-tagged) - Mouse cereblon (Crbn), transcript variant 2 |
CNY 6,370.00 |
