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Arhgap42 (NM_027823) Mouse Tagged ORF Clone
CAT＃: MR225433
- TrueORF®
Arhgap42 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 42 (Arhgap42)
ORF Plasmid: tGFP
"NM_027823" in other vectors (4)
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CNY 6,160.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9030420J04Rik; BE136619 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225433 representing NM_027823
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCCCACTCTGGAGTTCAGCGATTCGTATTTGGACAGCCCGGATTTTAGGGAGCGATTGCAGT GTCACGAGATCGAGCTGGAGAGAACCAACAAGTTCATCAAAGAGCTGCTGAAGGATGGCTCTCTGCTCAT CGGGGCGCTGAGAAATCTGTCCATGGCTGTACAGAAGTTTTCCCAGTCTCTGCAAGACTTCCAGTTTGAG TGTATTGGAGATGCAGAAACTGATGATGAAATTAGTATTGCTCAGTCACTGAAAGAATTCGCAAGGCTAC TTATTGCTGTAGAAGAAGAAAGGCGTAGACTGATTCAAAATGCTAATGATGTATTAATTGCACCCCTTGA GAAATTTCGAAAAGAACAGATCGGTGCAGCTAAAGCAGATTCACAGATTGGCCGAGAACATCAGAACTTC TATGAAGCTTCATTAGAATATGTCTTTAAGATCCAAGAAGTTCAAGAGAAGAAGAAGTTTGAGTTTGTGG AACCGCTTTTGTCCTTCCTTCAGGGTTTGTTTACATTTTACCACGAGGGATATGAACTTGCCCAGGAATT TGCACCGTACAAGCAACAGCTACAATTCAACTTGCAGAATACAAGGAATAACTTTGAAAGCACTCGGCAA GAAGTGGAGAGACTGATGCAGAGGATGAAGTCTGCCAATCAAGATTATAGACCTCCCAGCCAGTGGACCA TGGAGGGCTATCTTTACGTCCAGGAGAAAAGACCACTGGGTTTCACATGGACTAAACACTACTGTACTTA CGACAAAGGAAGCAAAATGTTCACAATGAGCGTCTCCGATGTGAAAGCCAGTGGGAAGATGAACGGCCTT GTTACTGGTTCGCCTGAAATGTTTAAATTAAAGTCGTGTATCCGACGGAAGACAGATTCCATTGACAAGC GCTTCTGCTTTGACATAGAAGTGGTCGAAAGGCATGGCATCATTACGCTACAGGCATTCTCTGAAGCTAA CCGGAAGCTCTGGCTGGAGGCCATGGATGGGAAAGAACCGATTTACACGTTACCCGCCATAATTAGCAAG AAAGAAGAAATGTACTTAAATGAAGCAGGCTTCAACTTTGTGAGAAAATGCATTCAAGCTGTAGAAATGA GAGGCATCACCATCTTGGGCCTGTACCGAATAGGAGGAGTGAACTCCAAGGTCCAGAAACTCATGAACAC CACATTTTCTCCCAAGTCCCCTCCTGATATGGACATTGATATTGAGCTGTGGGACAATAAGACTATAACG AGTGGGCTGAAAAACTACCTCAGGTGCCTTGCAGAACCACTAATGACTTACAAATTGCACAAGGATTTCA TCATTGCTGTCAAGTCAGACGACCAAAACTACAGGGTGGAGGCAGTCCATGCACTGGTACACAAATTGCC GGAGAAAAACAGAGAGATGCTGGACATCTTGATAAAACACCTTCTCAAAGTATCACTACATAGCCAACAA AATCTTATGACCATCTCAAATCTCGGTGTCATATTTGGTCCAACCCTGATGAGAGCACAGGAGGAAACTG TGGCTGCCATGATGAACATCAAATTTCAGAACATAGTGGTGGAAATCCTAATCGAGCACTATGAGAAGAT TTTTCATACTGCCCCAGACCCCAACATTCCTCTTCCTCAGCCTCAGTCTCGATCGGGATCCCGAAGAACC CGGGCCATCTGCCTGTCCACAGGTTCTCGGAAGCCCAGAGGGAGGTACACGCCGTGCCTGGCAGAGCCTG ATAGTGACTCCTACAGCAGCAGCCCAGACAGCACTCCCATGGGCAGCATAGAGTCGCTCTCCTCCCACTC CTCTGAACAAAACAGCACCACAAAATCCACTGCCTGCCAGCCCAGGGAGAAGTCAGGAGGAATTCCCTGG ATCACAACCCCATCATCTTCCAATGGACAGAAAAGTCAGGGTCTCTGGACAACTAGTCCAGAATCAAGTT CTCGAGAAGATGCAACCAAAACAGATGTGGAGTCAGATTGCCAGAGTGTGGCCTCTATCACAATCCCGGG GAATGTCTCTCCCCCCATAGACCTAGTCAAAAAAGGGCCCTATGGGCTCTCAGGACTGAAAAGATCCTCT GCTTCTTCCTCTCTCAGATCCATCTCTGCAGCTGAAGGAAACAAGAGCTACAGTGGATCCATTCAAAGCT TAACTTCTATAGGCTCCAAAGAGTCACCTAAAGCCATACCAAACCCAGAGCTGCCTCCAAAAATGTGCAG GAGGTTAAGGCTAGACACTGCCTCCAGCAATGGCTACCAGCGACCTGGCTCAGTAGTGGCAGCAAAGGCT CAACTCTTTGAAAATGCTGGGTCACCCAAGCCAGTTTCTTCTGGACGCCAAGCCCAAGCCATGTACTCCT GCAAAGCCGAGCACAGCCATGAGCTGTCCTTCCCCCAGGGGGCCATTTTTTCCAATGTACACCCGTCGGT GGAGCCAGGGTGGCTAAAGGCAACTTATGAAGGCAGAACAGGACTAGTCCCTGAAAACTACGTTGTCTTC CTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225433 representing NM_027823
Red=Cloning site Green=Tags(s) MGLPTLEFSDSYLDSPDFRERLQCHEIELERTNKFIKELLKDGSLLIGALRNLSMAVQKFSQSLQDFQFE CIGDAETDDEISIAQSLKEFARLLIAVEEERRRLIQNANDVLIAPLEKFRKEQIGAAKADSQIGREHQNF YEASLEYVFKIQEVQEKKKFEFVEPLLSFLQGLFTFYHEGYELAQEFAPYKQQLQFNLQNTRNNFESTRQ EVERLMQRMKSANQDYRPPSQWTMEGYLYVQEKRPLGFTWTKHYCTYDKGSKMFTMSVSDVKASGKMNGL VTGSPEMFKLKSCIRRKTDSIDKRFCFDIEVVERHGIITLQAFSEANRKLWLEAMDGKEPIYTLPAIISK KEEMYLNEAGFNFVRKCIQAVEMRGITILGLYRIGGVNSKVQKLMNTTFSPKSPPDMDIDIELWDNKTIT SGLKNYLRCLAEPLMTYKLHKDFIIAVKSDDQNYRVEAVHALVHKLPEKNREMLDILIKHLLKVSLHSQQ NLMTISNLGVIFGPTLMRAQEETVAAMMNIKFQNIVVEILIEHYEKIFHTAPDPNIPLPQPQSRSGSRRT RAICLSTGSRKPRGRYTPCLAEPDSDSYSSSPDSTPMGSIESLSSHSSEQNSTTKSTACQPREKSGGIPW ITTPSSSNGQKSQGLWTTSPESSSREDATKTDVESDCQSVASITIPGNVSPPIDLVKKGPYGLSGLKRSS ASSSLRSISAAEGNKSYSGSIQSLTSIGSKESPKAIPNPELPPKMCRRLRLDTASSNGYQRPGSVVAAKA QLFENAGSPKPVSSGRQAQAMYSCKAEHSHELSFPQGAIFSNVHPSVEPGWLKATYEGRTGLVPENYVVF L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027823 |
| ORF Size | 2523 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027823.2 |
| RefSeq Size | 5404 bp |
| RefSeq ORF | 2526 bp |
| Locus ID | 71544 |
| UniProt ID | B2RQE8 |
| MW | 95.1 kDa |
| Gene Summary | May influence blood pressure by functioning as a GTPase-activating protein for RHOA in vascular smooth muscle.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222007 | Arhgap42 (untagged) - Mouse Rho GTPase activating protein 42 (Arhgap42), (10ug) |
CNY 10,740.00 |
|
| MG225433 | Arhgap42 (tGFP-tagged) - Mouse Rho GTPase activating protein 42 (Arhgap42), (10ug) |
CNY 8,170.00 |
|
| MR225433L3 | Lenti ORF clone of Arhgap42 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 42 (Arhgap42) |
CNY 9,310.00 |
|
| MR225433L4 | Lenti ORF clone of Arhgap42 (mGFP-tagged) - Mouse Rho GTPase activating protein 42 (Arhgap42) |
CNY 9,310.00 |
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