Pak1 (NM_011035) Mouse Tagged ORF Clone
CAT#: MR225277
- TrueORF®
Pak1 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_011035" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,048.00
CNY 4,750.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW045634; PAK-1; Paka |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225277 representing NM_011035, codon optimized.
Due to the complexity of NM_011035, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAATAACGGCGTTGATATACAGGACAAACCACCCGCCCCTCCCATGAGGAATACATCCACCATGA TCGGCGCCGGCAGCAAGGATACGGGTACTCTCAACCATGGCTCTAAGCCCCTGCCGCCAAATCCCGAAGA AAAAAAAAAGAAGGATAGATTCTATAGAAGCATTCTGCCCGGGGATAAGACAAACAAGAAGAAAGAGAAG GAACGCCCGGAAATCAGTCTCCCTTCTGATTTTGAGCACACAATTCATGTTGGCTTCGATGCAGTAACCG GAGAGTTTACAGGGATGCCGGAGCAGTGGGCCAGGCTGCTGCAGACATCCAATATCACAAAGAGCGAGCA GAAAAAGAACCCACAGGCAGTGCTCGACGTGCTGGAGTTCTACAACAGTAAAAAGACCTCAAACAGTCAA AAGTACATGAGTTTTACCGACAAGAGTGCAGAGGATTACAACTCCTCCAACACACTGAACGTGAAGACAG TTTCAGAAACCCCAGCCGTTCCCCCAGTATCTGAGGACGAAGATGATGACGATGATGCCACACCACCACC CGTTATCGCCCCTAGGCCCGAGCACACTAAATCAGTTTATACCAGAAGTGTGATTGAACCGCTCCCGGTC ACCCCTACCCGAGATGTGGCTACCTCACCCATCAGTCCTACCGAAAACAACACTACCCCACCGGATGCCC TGACTCGAAACACTGAGAAACAGAAGAAGAAGCCAAAAATGTCCGATGAAGAGATTCTGGAGAAGCTGAG GTCTATTGTGAGTGTGGGGGACCCCAAGAAGAAGTACACCCGGTTCGAAAAGATCGGCCAGGGAGCAAGC GGCACTGTGTACACCGCCATGGACGTCGCCACTGGACAAGAAGTAGCAATCAAGCAAATGAATCTGCAGC AGCAGCCAAAGAAGGAACTGATCATTAATGAGATTCTTGTGATGCGGGAAAACAAAAACCCAAATATTGT GAACTACCTCGATAGTTATCTGGTAGGCGATGAGCTCTGGGTGGTGATGGAATACTTGGCTGGTGGATCA CTCACAGACGTCGTGACCGAAACCTGTATGGACGAGGGTCAGATTGCTGCGGTCTGTAGAGAGTGCTTGC AGGCTCTGGAGTTTCTGCACAGCAACCAAGTGATCCATCGCGACATCAAAAGCGACAACATCTTGCTCGG TATGGATGGGAGTGTCAAGCTCACAGACTTCGGATTTTGTGCGCAGATCACGCCCGAGCAGAGCAAAAGA AGCACAATGGTGGGGACCCCCTACTGGATGGCCCCTGAAGTTGTTACACGCAAGGCTTACGGACCAAAGG TAGACATTTGGTCACTGGGTATTATGGCTATAGAGATGATTGAGGGAGAACCACCATACCTGAACGAGAA CCCACTCCGGGCCCTGTACCTGATCGCTACTAATGGGACACCAGAACTGCAGAACCCCGAGAAACTCTCT GCGATCTTTCGAGATTTCCTGAATCGCTGCCTTGAGATGGATGTGGAAAAAAGGGGTAGCGCTAAAGAGC TGCTTCAGCATCAGTTCCTTAAAATCGCCAAACCACTGTCAAGCCTTACTCCATTGATCGCTGCAGCTAA GGAGGCTACCAAAAATAACCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225277 representing NM_011035
Red=Cloning site Green=Tags(s) MSNNGVDIQDKPPAPPMRNTSTMIGAGSKDTGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEK ERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQ KYMSFTDKSAEDYNSSNTLNVKTVSETPAVPPVSEDEDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPV TPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGAS GTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGS LTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKR STMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS AIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_011035 |
ORF Size | 1632 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011035.2, NP_035165.2 |
RefSeq Size | 3081 bp |
RefSeq ORF | 1635 bp |
Locus ID | 18479 |
MW | 60.6 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218214 | Pak1 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1), (10ug) |
CNY 6,940.00 |
|
MG225277 | Pak1 (tGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1), (10ug). Note: ORF is codon optimized |
CNY 5,230.00 |
|
MR225277L3 | Lenti ORF clone of Pak1 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1). Note: ORF is codon optimized |
CNY 6,650.00 |
|
MR225277L4 | Lenti ORF clone of Pak1 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1). Note: ORF is codon optimized |
CNY 6,650.00 |