Axin1 (NM_001159598) Mouse Tagged ORF Clone
CAT#: MR225160
- TrueORF®
Axin1 (Myc-DDK-tagged) - Mouse axin 1 (Axin1), transcript variant 1
ORF Plasmid: tGFP
"NM_001159598" in other vectors (4)
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CNY 8,270.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI316800; Axin; Fu; fused; Kb; Ki; kinky; knobbly |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225160 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGTCCCAAAATGAATGTCCAGGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAG ATGCCCCCCGGCCCCCAGTGCCTGGAGAAGAGGGAGAACTGGTATCTACTGATTCGAGGCCTGTCAACCA CAGTTTCTGTTCTGGGAAAGGTACCAGCATTAAAAGTGAGACCTCAACAGCCACCCCAAGACGTTCAGAT CTGGATCTGGGATATGAGCCCGAGGGCAGTGCCTCCCCCACCCCACCATATTTGAGGTGGGCTGAGTCAC TGCATTCCTTACTGGATGACCAAGATGGGATCAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGC TGACCTGCTGGACTTCTGGTTTGCCTGCAGTGGCTTCAGGAAGCTTGAGCCCTGTGACTCAAATGAGGAA AAGAGGCTGAAGCTGGCAAGAGCCATCTACCGAAAGTACATCCTGGATAGCAATGGCATTGTGTCCAGAC AAACCAAGCCAGCCACTAAGAGCTTCATAAAGGACTGTGTCATGAAGCAGCAGATAGATCCTGCCATGTT TGACCAGGCACAGACAGAAATCCAGTCCACCATGGAGGAGAATACCTACCCTTCCTTTCTTAAGTCTGAC ATTTATTTGGAGTACACAAGGACAGGCTCAGAGAGTCCGAAGGTCTGCAGTGACCAGAGCTCAGGGTCTG GAACAGGGAAGGGCATGTCTGGATACCTGCCCACTTTGAATGAGGATGAAGAATGGAAATGTGACCAAGA TGCAGATGAGGATGATGGCCGAGACCCTCTCCCCCCCAGCAGGCTCACCCAGAAGCTGCTATTGGAGACT GCTGCCCCGAGGGCCCCCTCAAGTAGACGGTACAACGAAGGCAGAGAGCTCAGGTATGGATCTTGGAGGG AGCCCGTCAACCCCTACTACGTCAACTCTGGCTATGCCCTGGCCCCAGCCACCAGTGCCAATGACAGTGA GCAGCAGAGCCTGTCCAGTGATGCTGACACGCTATCCCTTACGGACAGTAGTGTGGATGGAATCCCCCCA TACAGGATCCGTAAGCAGCACCGAAGGGAGATGCAGGAGAGTATCCAAGTCAATGGGCGGGTACCTCTAC CTCACATTCCTCGCACTTACCGAATGCCAAAGGAGATCCGGGTAGAGCCACAGAAATTTGCTGAAGAGCT TATTCACCGTCTAGAGGCTGTCCAGCGCACTCGAGAGGCTGAAGAAAAGTTGGAGGAACGGCTGAAGCGT GTACGCATGGAGGAAGAAGGGGAGGATGGTGAAATGCCTTCTGGCCCCATGGCAAGTCACAAGCTGCCTT CTGTCCCAGCTTGGCACCATTTCCCACCCCGCTATGTGGATATGGGCTGCTCTGGACTGCGGGATGCCCA TGAGGAGAATCCTGAGAGCATCCTGGATGAGCACGTGCAAAGGGTCATGAGGACACCTGGCTGCCAGTCA CCTGGCCCAGGCCACCGCTCTCCTGACAGTGGGCATGTGGCTAAGACTGCAGTGCTAGGGGGTACAGCCT CCGGGCATGGGAAGCATGTTCCTAAGTTAGGGTTGAAGCTGGATACAGCTGGCCTGCACCATCATAGACA TGTCCACCACCATGTTCACCATAATTCAGCTAGACCTAAGGAGCAAATGGAGGCTGAAGTTGCCCGCAGG GTCCAGAGCAGCTTCTCGTGGGGCCCAGAAACACATGGTCATGCCAAGCCCCGGAGCTATTCCGAGAACG CAGGCACCACCCTCAGTGCTGGGGATTTGGCCTTTGGTGGTAAAACTAGTGCACCTTCCAAAAGAAACAC CAAGAAGGCTGAATCTGGGAAGAATGCCAATGCTGAGGTACCCAGTACCACAGAGGACGCTGAGAAGAAC CAGAAGATCATGCAGTGGATCATTGAGGGAGAGAAGGAGATCAGTAGACACCGGAAGGCAGGCCATGGGT CTTCTGGGTTGAGGAAGCAGCAGGCCCATGAAAGCTCCAGGCCCTTGTCCATCGAGCGTCCTGGGGCCGT GCACCCCTGGGTCAGCGCTCAGCTTCGGAATTCTGTCCAGCCTTCTCATCTTTTCATCCAAGATCCCACA ATGCCACCCAATCCAGCCCCTAATCCCCTGACCCAGCTGGAAGAGGCCCGCAGGCGTTTGGAAGAAGAAG AAAAGAGAGCAAACAAACTGCCCTCCAAGCAGAGGACAAAATCACAAAGAAAGGCAGGTGGCGGGAGTGC ACCACCATGTGACAGCATTGTTGTGGCCTACTATTTCTGTGGGGAACCCATCCCCTACCGGACCCTGGTG AGGGGCCGTGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTAACCAAGAAGGGGAGCTACAGATACTACT TTAAGAAAGTGAGTGATGAGTTTGACTGTGGTGTGGTATTTGAGGAAGTACGGGAGGATGAGGCCGTCTT GCCTGTCTTTGAAGAAAAGATCATCGGCAAGGTGGAAAAGGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225160 protein sequence
Red=Cloning site Green=Tags(s) MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSD LDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEE KRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSD IYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLET AAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPP YRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKR VRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQS PGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARR VQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKN QKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPT MPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLV RGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159598 |
ORF Size | 2496 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159598.1, NP_001153070.1 |
RefSeq Size | 3671 bp |
RefSeq ORF | 2499 bp |
Locus ID | 12005 |
MW | 93 kDa |
Gene Summary | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221928 | Axin1 (untagged) - Mouse axin 1 (Axin1), transcript variant 1, (10ug) |
CNY 10,640.00 |
|
MG225160 | Axin1 (tGFP-tagged) - Mouse axin 1 (Axin1) transcript variant 1, (10ug) |
CNY 8,304.00 |
|
MR225160L3 | Lenti ORF clone of Axin1 (Myc-DDK-tagged) - Mouse axin 1 (Axin1), transcript variant 1 |
CNY 10,170.00 |
|
MR225160L4 | Lenti ORF clone of Axin1 (mGFP-tagged) - Mouse axin 1 (Axin1), transcript variant 1 |
CNY 10,170.00 |