Unc13a (NM_001029873) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410078G03Rik; Munc13-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR225156 representing NM_001029873 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCTGTGCGTGGGAGTCAAAAAAGCCAAGTTTGACGGTGCCCAAGAGAAGTTCAACACATACG TGACGCTGAAGGTGCAGAACGTGAAGAGCACTACCATAGCTGTACGTGGCAGCCAGCCCAGCTGGGAGCA GGACTTCATGTTTGAGATCAACCGCCTGGATCTAGGCCTGACCGTGGAGGTGTGGAACAAGGGTCTCATC TGGGACACGATGGTGGGTACTGTGTGGATCCCGCTGCGGACCATCCGCCAGTCCAATGAGGAGGGCCCAG GAGAGTGGCTCACACTGGACTCTCAGGCCATCATGGCGGACAGTGAGATCTGTGGGACCAAGGACCCCAC CTTCCATCGCATCCTCCTGGACGCTCATTTCGAGCTGCCGTTGGACATCCCTGAGGAGGAGGCACGCTAC TGGGCCAAGAAGCTGGAGCAGCTGAATGCCATGCGTGACCAGGATGAGTACTCCTTCCAGGACCAGCAGG ACAAGCCGCTGCCAGTGCCCAGCAGCCAGTGCTGCAACTGGAATTACTTTGGCTGGGGAGAACAGAATGA CGACCCCGACAGTGCGGTGGATGACCGGGACAGTGACTATAGGAGTGAGACCAGCAACAGCATCCCACCG CCCTACTATACGACTTCGCAGCCCAACGCCTCTGTGCACCAGTACTCCGTGCGACCGCCCCCTCTGGGGT CCCGGGAGTCCTACAGTGACTCCATGCACAGCTATGAGGAGTTCTCTGAGCCGCGGGCGCTCAGCCCCAC AGGCAGCAGCCGCTATGCTTCTTCCGGGGAGCTGAGCCAGGGAAGCTCACAGCTGAGCGAGGACTTCGAC CCTGATGAGCACAGCCTGCAGGGCTCGGAGCTGGATGACGAGAGGGACCGCGATTCTTATCACTCCTGTC ACAGCTCCGTGAGTTACCACAAGGACTCACCCCGCTGGGACCAGGACGATGAGGACCTGGAGGACCTGGA GGACCTGGAAGATGAGGAGCTGCCCGAGGAGGAGGAGGAGTTGGAGGAGGAGGGGGAGGAGGAATTGGAG GAAGAGGATTTGGAGGAGGAGGAGGAGGTGCCTGATGACCTGGCCAGCTACACCCAGCAGGAGGACACCA CTGTGGCTGAGCCCAAAGAGTTCAAGCGGATCAGCTTCCCAACGGCTGCGCCTCAGAAGGATGACAAGGT TTCAGCTGTGCCTACAGAGGCCCCCGAGGTGGCCAAAGGCATCCCCAAAGCAGCCACACCCGAGGAGAAG GCAGCTGCAGAGCGTGCGCAGGAAGCCGAGCCCCCCAAGTCTGAGGAGAGTTTCAGATCTCGAGAGGAGG AAGAGGGCCAGGAAGGGCAGGATGCCATGTCCAGAGCCAAAGCCAACTGGTTGCGAGCCTTCAACAAGGT GCGCATGCAGCTGCAGGAGGCCCGAGGAGAAGGGGATATGTCTAAGTCTCTATGGTTCAAAGGCGGTCCT GGTGGTGGCCTTATCATCATTGACAGCATGCCAGACATCAGGAAACGGAAGCCCATTCCCCTCGTGAGCG ACCTGGCTATGTCATTGGTGCAGTCTCGGAAGGCAGGCATCACCTCGGCCTTGGCCTCCAGCACGTTGAA CAATGAAGAGCTGAAAAATCACGTTTACAAGAAGACCCTGCAAGCCTTAATCTACCCCATCTCCTGCACC ACGCCGCACAATTTCGAGGTGTGGACGGCCACCACGCCCACCTACTGCTATGAGTGCGAGGGGCTGCTGT GGGGCATCGCGCGGCAGGGCATGCGATGCACCGAGTGCGGCGTTAAGTGCCACGAGAAGTGCCAGGACCT GCTCAATGCGGATTGCCTGCAGCGGGCGGCTGAGAAGAGTTCCAAGCATGGCGCTGAAGACCGCACGCAG AACATCATCATGGTGCTGAAGGACCGCATGAAGATCCGCGAGCGCAACAAGCCTGAGATCTTCGAGCTTA TCCAAGAGATCTTCGCGGTCACCAAGAGCGCACACACACAGCAGATGAAAGCTGTCAAGCAGAGTGTGCT GGACGGCACATCCAAGTGGTCTGCCAAAATTAGCATCACAGTGGTCTGTGCCCAGGGCTTGCAGGCCAAG GACAAGACAGGATCCAGTGACCCTTATGTCACCGTCCAGGTTGGGAAGACCAAGAAAAGGACAAAAACCA TCTACGGGAACCTCAACCCAGTGTGGGAAGAGAATTTTCATTTTGAATGTCACAACTCCTCTGACCGGAT CAAGGTTCGTGTTTGGGATGAAGATGACGACATAAAATCCCGTGTGAAACAAAGGTTTAAGAGGGAGTCT GATGACTTCCTAGGGCAGACAATCATCGAGGTGCGGACGCTTAGCGGCGAGATGGATGTGTGGTACAATC TGGACAAGAGAACGGACAAGTCGGCGGTGTCGGGGGCCATCCGGCTTCACATCAGTGTGGAGATCAAAGG GGAGGAGAAGGTGGCGCCCTACCATGTCCAGTACACCTGTCTGCATGAGAACCTGTTCCACTTTGTGACG GACGTGCAGAACAATGGTGTGGTGAAGATTCCCGATGCCAAGGGTGACGACGCCTGGAAGGTTTACTACG ATGAGACGGCCCAGGAGATCGTGGATGAGTTTGCCATGCGCTATGGTGTTGAGTCCATCTACCAAGCCAT GACCCACTTTGCCTGCCTCTCCTCCAAGTATATGTGCCCTGGGGTACCCGCTGTCATGAGCACCCTGCTT GCCAACATCAACGCCTACTACGCACACACCACCGCCTCCACCAACGTGTCTGCCTCTGACCGCTTCGCTG CCTCTAATTTCGGGAAAGAGCGCTTTGTGAAACTTCTGGACCAGCTACACAATTCTCTGCGGATCGACCT GTCCATGTACCGGAACAACTTCCCAGCCAGCAGCCCTGAGCGGCTGCAGGATCTCAAGTCCACGGTGGAC CTGCTCACCAGCATCACCTTCTTCCGGATGAAGGTTCAGGAACTGCAGAGCCCGCCGCGTGCCAGCCAGG TAGTGAAAGACTGTGTGAAGGCCTGCCTCAACTCCACCTATGAGTACATCTTCAACAACTGCCATGAGCT CTATGGCCGGGAGTACCAAACGGATCCGGCCAAGAAGGGGGAGGTTCCCCCAGAGGAGCAAGGCCCTAGC ATCAAGAACCTGGATTTCTGGTCCAAGCTGATCACCCTCATCGTGTCTATCATCGAGGAGGATAAGAATT CTTACACACCCTGCCTCAATCAGTTTCCCCAGGAGCTCAATGTGGGGAAGATCAGTGCTGAGGTGATGTG GAGCCTATTTGCCCAAGACATGAAGTACGCCATGGAGGAACATGACAAACACCGGCTGTGTAAGAGCGCA GATTACATGAACCTGCACTTCAAGGTGAAGTGGCTGTACAATGAGTACGTGGCCGAGCTGCCCACCTTCA AGGACCGTGTGCCTGAGTACCCTGCGTGGTTTGAGCCCTTCGTCATCCAGTGGTTGGATGAGAATGAGGA GGTGTCCCGGGACTTCCTGCATGGTGCCCTCGAGCGGGACAAGAAGGACGGGTTCCAGCAGACCTCAGAG CACGCCCTGTTCTCTTGCTCAGTGGTGGACGTCTTCTCCCAGCTTAACCAGAGTTTCGAGATCATCAAGA AGCTGGAGTGTCCTGACCCCCAGATCGTGGGCCATTACATGCGGCGTTTTGCCAAGACCATTAGCAATGT GCTTCTCCAGTATGCAGACATCGTTTCCAAGGACTTCGCCTCCTACTGCTCCAAGGAGAAGGAGAAAGTG CCCTGCATCCTCATGAACAACACACAGCAACTACGAGTGCAGCTGGAGAAGATGTTTGAGGCGATGGGCG GGAAGGAGCTGGATGCCGAGGCCAGTGGCACCCTGAAGGAGCTGCAGGTGAAACTCAACAATGTCCTGGA TGAACTCAGCCATGTGTTTGCCACCAGCTTCCAGCCACACATTGAGGAATGCGTCAGACAGATGGGTGAC ATCCTGAGCCAGGTGAAGGGCACAGGCAATGTACCTGCCAGTGCCTGCAGCAGCGTGGCCCAGGATGCAG ACAATGTGCTGCAGCCCATCATGGATCTTCTGGACAGCAACCTCACCCTGTTTGCCAAAATCTGTGAGAA GACAGTTCTGAAGCGGGTGCTGAAGGAGCTCTGGAAGCTGGTGATGAACACAATGGAGAAGACCATCGTC CTGCCACCGCTCACTGACCAGACGATGATTGGCACCCTCTTGAGAAAACATGGCAAGGGCCTAGAAAAGG GCAGGGTGAAACTGCCAAGCCACTCAGACGGGACACAAATGATCTTCAATGCTGCCAAGGAGCTGGGCCA GCTGTCCAAACTTAAGGATCACATGGTGCGGGAAGAAGCCAAGAGCTTGACCCCGAAGCAGTGTGCTGTT GTTGAACTGGCCCTGGACACCATCAAGCAATACTTCCACGCGGGGGGCGTTGGCCTCAAGAAGACCTTCC TGGAGAAGAGCCCGGACCTCCAGTCCCTGCGCTACGCCCTGTCGCTCTACACGCAAGCCACCGACCTGCT CATCAAAACCTTCGTGCAGACGCAGTCAGCGCAGGGCTCTGGTGTGGAGGACCCTGTAGGTGAAGTGTCC GTCCACGTGGAGCTGTTCACACATCCAGGAACTGGGGAACAGAAGGTCACAGTGAAGGTGGTGGCCGCCA ATGACCTCAAGTGGCAGACTTCTGGCATCTTCCGCCCGTTCATCGAGGTCAACATCGTTGGACCTCAGCT TAGCGACAAGAAACGCAAGTTCGCCACCAAATCCAAAAACAACAGCTGGGCGCCCAAGTATAACGAGAGC TTCCAGTTCTCCCTGAGCGCCGACGCGGGCCCCGAGTGCTACGAGCTGCAGGTGTGCGTGAAGGACTACT GCTTTGCGCGCGAAGACCGCACGGTGGGGCTGGCGGTGCTGCAACTGCGAGAGCTGGCCCAGCGCGGGAG CGCCGCGTGCTGGCTTCCACTCGGCCGCCGCATCCACATGGACGACACGGGGCTCACAGTGCTGCGCATC CTGTCGCAGCGCAGCAACGATGAGGTGGCCAAGGAATTCGTCAAGCTCAAGTCGGACACGCGCTCAGCCG AGGAGGGCGGTGCCGCGCCTGCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR225156 representing NM_001029873 Red=Cloning site Green=Tags(s) MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLI WDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARY WAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPP PYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFD PDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDDEDLEDLEDLEDEELPEEEEELEEEGEEELE EEDLEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKDDKVSAVPTEAPEVAKGIPKAATPEEK AAAERAQEAEPPKSEESFRSREEEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGDMSKSLWFKGGP GGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCT TPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQ NIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAK DKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRES DDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVT DVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLL ANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVD LLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPS IKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSA DYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSE HALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKV PCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGD ILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIV LPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAV VELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQGSGVEDPVGEVS VHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNES FQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRI LSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001029873
ORF Size	5136 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001029873.2, NP_001025044.2
RefSeq Size	7983 bp
RefSeq ORF	5139 bp
Locus ID	382018
UniProt ID	Q4KUS2
MW	193.8 kDa
Gene Summary	Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]

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