Unc13a (NM_001029873) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410078G03Rik; Munc13-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224865 representing NM_001029873 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCTGTGCGTGGGAGTCAAAAAAGCCAAGTTTGACGGTGCCCAAGAGAAGTTCAACACATACG TGACGCTGAAGGTGCAGAACGTGAAGAGCACTACCATAGCTGTACGTGGCAGCCAGCCCAGCTGGGAGCA GGACTTCATGTTTGAGATCAACCGCCTGGATCTAGGCCTGACCGTGGAGGTGTGGAACAAGGGTCTCATC TGGGACACGATGGTGGGTACTGTGTGGATCCCGCTGCGGACCATCCGCCAGTCCAATGAGGAGGGCCCAG GAGAGTGGCTCACACTGGACTCTCAGGCCATCATGGCGGACAGTGAGATCTGTGGGACCAAGGACCCCAC CTTCCATCGCATCCTCCTGGACGCTCATTTCGAGCTGCCGTTGGACATCCCTGAGGAGGAGGCACGCTAC TGGGCCAAGAAGCTGGAGCAGCTGAATGCCATGCGTGACCAGGATGAGTACTCCTTCCAGGACCAGCAGG ACAAGCCGCTGCCAGTGCCCAGCAGCCAGTGCTGCAACTGGAATTACTTTGGCTGGGGAGAACAGAATGA CGACCCCGACAGTGCGGTGGATGACCGGGACAGTGACTATAGGAGTGAGACCAGCAACAGCATCCCACCG CCCTACTATACGACTTCGCAGCCCAACGCCTCTGTGCACCAGTACTCCGTGCGACCGCCCCCTCTGGGGT CCCGGGAGTCCTACAGTGACTCCATGCACAGCTATGAGGAGTTCTCTGAGCCGCGGGCGCTCAGCCCCAC AGGCAGCAGCCGCTATGCTTCTTCCGGGGAGCTGAGCCAGGGAAGCTCACAGCTGAGCGAGGACTTCGAC CCTGATGAGCACAGCCTGCAGGGCTCGGAGCTGGATGACGAGAGGGACCGCGATTCTTATCACTCCTGTC ACAGCTCCGTGAGTTACCACAAGGACTCACCCCGCTGGGACCAGGACGATGAGGACCTGGAGGACCTGGA GGACCTGGAAGATGAGGAGCTGCCCGAGGAGGAGGAGGAGTTGGAGGAGGAGGGGGAGGAGGAATTGGAG GAAGAGGATTTGGAGGAGGAGGAGGAGGTGCCTGATGACCTGGCCAGCTACACCCAGCAGGAGGACACCA CTGTGGCTGAGCCCAAAGAGTTCAAGCGGATCAGCTTCCCAACGGCTGCGCCTCAGAAGGATGACAAGGT TTCAGCTGTGCCTACAGAGGCCCCCGAGGTGGCCAAAGGCATCCCCAAAGCAGCCACACCCGAGGAGAAG GCAGCTGCAGAGCGTGCGCAGGAAGCCGAGCCCCCCAAGTCTGAGGAGAGTTTCAGATCTCGAGAGGAGG AAGAGGGCCAGGAAGGGCAGGATGCCATGTCCAGAGCCAAAGCCAACTGGTTGCGAGCCTTCAACAAGGT GCGCATGCAGCTGCAGGAGGCCCGAGGAGAAGGGGATATGTCTAAGTCTCTATGGTTCAAAGGCGGTCCT GGTGGTGGCCTTATCATCATTGACAGCATGCCAGACATCAGGAAACGGAAGCCCATTCCCCTCGTGAGCG ACCTGGCTATGTCATTGGTGCAGTCTCGGAAGGCAGGCATCACCTCGGCCTTGGCCTCCAGCACGTTGAA CAATGAAGAGCTGAAAAATCACGTTTACAAGAAGACCCTGCAAGCCTTAATCTACCCCATCTCCTGCACC ACGCCGCACAATTTCGAGGTGTGGACGGCCACCACGCCCACCTACTGCTATGAGTGCGAGGGGCTGCTGT GGGGCATCGCGCGGCAGGGCATGCGATGCACCGAGTGCGGCGTTAAGTGCCACGAGAAGTGCCAGGACCT GCTCAATGCGGATTGCCTGCAGCGGGCGGCTGAGAAGAGTTCCAAGCATGGCGCTGAAGACCGCACGCAG AACATCATCATGGTGCTGAAGGACCGCATGAAGATCCGCGAGCGCAACAAGCCTGAGATCTTCGAGCTTA TCCAAGAGATCTTCGCGGTCACCAAGAGCGCACACACACAGCAGATGAAAGCTGTCAAGCAGAGTGTGCT GGACGGCACATCCAAGTGGTCTGCCAAAATTAGCATCACAGTGGTCTGTGCCCAGGGCTTGCAGGCCAAG GACAAGACAGGATCCAGTGACCCTTATGTCACCGTCCAGGTTGGGAAGACCAAGAAAAGGACAAAAACCA TCTACGGGAACCTCAACCCAGTGTGGGAAGAGAATTTTCATTTTGAATGTCACAACTCCTCTGACCGGAT CAAGGTTCGTGTTTGGGATGAAGATGACGACATAAAATCCCGTGTGAAACAAAGGTTTAAGAGGGAGTCT GATGACTTCCTAGGGCAGACAATCATCGAGGTGCGGACGCTTAGCGGCGAGATGGATGTGTGGTACAATC TGGACAAGAGAACGGACAAGTCGGCGGTGTCGGGGGCCATCCGGCTTCACATCAGTGTGGAGATCAAAGG GGAGGAGAAGGTGGCGCCCTACCATGTCCAGTACACCTGTCTGCATGAGAACCTGTTCCACTTTGTGACG GACGTGCAGAACAATGGTGTGGTGAAGATTCCCGATGCCAAGGGTGACGACGCCTGGAAGGTTTACTACG ATGAGACGGCCCAGGAGATCGTGGATGAGTTTGCCATGCGCTATGGTGTTGAGTCCATCTACCAAGCCAT GACCCACTTTGCCTGCCTCTCCTCCAAGTATATGTGCCCTGGGGTACCCGCTGTCATGAGCACCCTGCTT GCCAACATCAACGCCTACTACGCACACACCACCGCCTCCACCAACGTGTCTGCCTCTGACCGCTTCGCTG CCTCTAATTTCGGGAAAGAGCGCTTTGTGAAACTTCTGGACCAGCTACACAATTCTCTGCGGATCGACCT GTCCATGTACCGGAACAACTTCCCAGCCAGCAGCCCTGAGCGGCTGCAGGATCTCAAGTCCACGGTGGAC CTGCTCACCAGCATCACCTTCTTCCGGATGAAGGTTCAGGAACTGCAGAGCCCGCCGCGTGCCAGCCAGG TAGTGAAAGACTGTGTGAAGGCCTGCCTCAACTCCACCTATGAGTACATCTTCAACAACTGCCATGAGCT CTATGGCCGGGAGTACCAAACGGATCCGGCCAAGAAGGGGGAGGTTCCCCCAGAGGAGCAAGGCCCTAGC ATCAAGAACCTGGATTTCTGGTCCAAGCTGATCACCCTCATCGTGTCTATCATCGAGGAGGATAAGAATT CTTACACACCCTGCCTCAATCAGTTTCCCCAGGAGCTCAATGTGGGGAAGATCAGTGCTGAGGTGATGTG GAGCCTATTTGCCCAAGACATGAAGTACGCCATGGAGGAACATGACAAACACCGGCTGTGTAAGAGCGCA GATTACATGAACCTGCACTTCAAGGTGAAGTGGCTGTACAATGAGTACGTGGCCGAGCTGCCCACCTTCA AGGACCGTGTGCCTGAGTACCCTGCGTGGTTTGAGCCCTTCGTCATCCAGTGGTTGGATGAGAATGAGGA GGTGTCCCGGGACTTCCTGCATGGTGCCCTCGAGCGGGACAAGAAGGACGGGTTCCAGCAGACCTCAGAG CACGCCCTGTTCTCTTGCTCAGTGGTGGACGTCTTCTCCCAGCTTAACCAGAGTTTCGAGATCATCAAGA AGCTGGAGTGTCCTGACCCCCAGATCGTGGGCCATTACATGCGGCGTTTTGCCAAGACCATTAGCAATGT GCTTCTCCAGTATGCAGACATCGTTTCCAAGGACTTCGCCTCCTACTGCTCCAAGGAGAAGGAGAAAGTG CCCTGCATCCTCATGAACAACACACAGCAACTACGAGTGCAGCTGGAGAAGATGTTTGAGGCGATGGGCG GGAAGGAGCTGGATGCCGAGGCCAGTGGCACCCTGAAGGAGCTGCAGGTGAAACTCAACAATGTCCTGGA TGAACTCAGCCATGTGTTTGCCACCAGCTTCCAGCCACACATTGAGGAATGCGTCAGACAGATGGGTGAC ATCCTGAGCCAGGTGAAGGGCACAGGCAATGTACCTGCCAGTGCCTGCAGCAGCGTGGCCCAGGATGCAG ACAATGTGCTGCAGCCCATCATGGATCTTCTGGACAGCAACCTCACCCTGTTTGCCAAAATCTGTGAGAA GACAGTTCTGAAGCGGGTGCTGAAGGAGCTCTGGAAGCTGGTGATGAACACAATGGAGAAGACCATCGTC CTGCCACCGCTCACTGACCAGACGATGATTGGCACCCTCTTGAGAAAACATGGCAAGGGCCTAGAAAAGG GCAGGGTGAAACTGCCAAGCCACTCAGACGGGACACAAATGATCTTCAATGCTGCCAAGGAGCTGGGCCA GCTGTCCAAACTTAAGGATCACATGGTGCGGGAAGAAGCCAAGAGCTTGACCCCGAAGCAGTGTGCTGTT GTTGAACTGGCCCTGGACACCATCAAGCAATACTTCCACGCGGGGGGCGTTGGCCTCAAGAAGACCTTCC TGGAGAAGAGCCCGGACCTCCAGTCCCTGCGCTACGCCCTGTCGCTCTACACGCAAGCCACCGACCTGCT CATCAAAACCTTCGTGCAGACGCAGTCAGCGCAGGGCTCTGGTGTGGAGGACCCTGTAGGTGAAGTGTCC GTCCACGTGGAGCTGTTCACACATCCAGGAACTGGGGAACAGAAGGTCACAGTGAAGGTGGTGGCCGCCA ATGACCTCAAGTGGCAGACTTCTGGCATCTTCCGCCCGTTCATCGAGGTCAACATCGTTGGACCTCAGCT TAGCGACAAGAAACGCAAGTTCGCCACCAAATCCAAAAACAACAGCTGGGCGCCCAAGTATAACGAGAGC TTCCAGTTCTCCCTGAGCGCCGACGCGGGCCCCGAGTGCTACGAGCTGCAGGTGTGCGTGAAGGACTACT GCTTTGCGCGCGAAGACCGCACGGTGGGGCTGGCGGTGCTGCAACTGCGAGAGCTGGCCCAGCGCGGGAG CGCCGCGTGCTGGCTTCCACTCGGCCGCCGCATCCACATGGACGACACGGGGCTCACAGTGCTGCGCATC CTGTCGCAGCGCAGCAACGATGAGGTGGCCAAGGAATTCGTCAAGCTCAAGTCGGACACGCGCTCAGCCG AGGAGGGCGGTGCCGCGCCTGCGCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001029873
Insert Size	5139 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001029873.2, NP_001025044.2
RefSeq Size	7983 bp
RefSeq ORF	5139 bp
Locus ID	382018
UniProt ID	Q4KUS2
Gene Summary	Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion. Plays a role in dendrite formation by melanocytes (By similarity).[UniProtKB/Swiss-Prot Function]

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