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Gucy2e (NM_008192) Mouse Tagged ORF Clone
CAT＃: MR224986
- TrueORF®
Gucy2e (Myc-DDK-tagged) - Mouse guanylate cyclase 2e (Gucy2e)
ORF Plasmid: tGFP
"NM_008192" in other vectors (4)
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CNY 7,712.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GC-E; GC1; ROS-GC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224986 representing NM_008192
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCTTGGCTCCTGCCAGCCGGAGGGCTTCCCGGCGCCGGGTTCTGTGTCCCTGCGCGGCAGTCTC CGTCCAGTTTCTCGCGGGTCCTGCGCTGGCCAAGGCCTGGGCTACCGGGACTCCTGCTACTGCTACTGCT CCCATCTCCTTCTGCCCTCTCTGCTGTGTTCAAAGTGGGGGTGCTGGGCCCCTGGGCTTGCGACCCCATC TTTGCACGGGCCCGACCAGACCTGGCTGCGCGTCTGGCCGCCAACCGCCTGAATCGTGACTTTGCTTTAG ACGGCGGCCCCCGGTTCGAGGTTGCGCTGCTCCCAGAGCCCTGCCTGACTCCGGGCTCACTAGGGGCTGT GTCCTCTGCGCTGTCTCGAGTCTCTGGCCTGGTGGGTCCGGTGAACCCCGCAGCCTGTCGGCCAGCCGAA CTGTTGGCTCAAGAAGCTGGAGTAGCGCTGGTGCCCTGGGGCTGCCCTGGCACGCGGGCGGCGGGTACTA CAGCCCCGGCGGTGACCCCCGCTGCAGATGCTCTCTACGTCCTCCTTAGAGCATTCCGCTGGGCGCGCGT GGCCCTGATCACTGCACCCCAAGACCTGTGGGTGGAGGCGGGACGCGCTCTGTCCACAGCACTCAGGGCC CGGGGTTTGCCAGTTGCCCTAGTGACTTCCATGGAGACTTCAGACCGGTCTGGAGCCCGGGAGGCCCTCG GGAGGATCCGAGATGGGCCTAGAGTTAGAGTAGTGATCATGGTGATGCACTCGGTGCTGCTGGGCGGCGA GGAGCAGCGCTACCTACTGGAAGCTGCAGAAGAACTGGCTCTGACTGATGGCTCCCTGGTTTTCCTGCCC TTCGACACGCTTCACTACGCTTTGTCTCCAGGCCCGGAGGCTCTGGCTGCATTTGTCAACAGCTCCCAGC TCCGCAGGGCTCACGATGCGGTGCTCACACTCACGCGCCGCTGTCCTCCTGGAGGCAGCGTGCAAGACAG CCTGCGCAGGGCTCAAGAACACCAGGAACTGCCCCTTGACCTCAACCTGAAGCAGGTCTCTCCGCTGTTT GGCACCATCTATGATGCTGTCTTCCTGTTGGCTGGGGGCGTGAAGAGAGCAAGAACAGCGGTGGGTGGTG GCTGGGTGTCAGGTGCATCTGTAGCCCGCCAAGTACGGGAAGCACAAGTCTCTGGCTTTTGTGGGGTCCT GGGAAGAACCGAGGAGCCCTCCTTTGTGCTGCTGGACACAGATGCATCCGGAGAACAGTTGTTCGCAACA CACCTGCTAGATCCTGTCTTAGGCTCCCTGCGTTCTGCAGGGACCCCCATGCACTTCCCTAGAGGTGGAC CTGCCCCGGGACCAGACCCTTCCTGCTGGTTCGATCCAGATGTGATCTGCAACGGAGGGGTGGAGCCAGG CCTGGTCTTTGTTGGCTTCCTCCTGGTGATAGGGATGGGACTGACTGGAGCCTTCTTGGCTCATTACTTG AGGCACAGGCTGCTACACATGCAGATGGCTTCCGGCCCCAACAAGATCATCTTGACGTTGGAAGATGTTA CTTTCCTCCACCCACCGGGAGGCAGCTCTCGAAAGGTGGTCCAGGGAAGTAGATCCAGTCTGGCTACCCG GAGCGCATCAGACATTCGCAGTGTCCCCAGCCAGCCCCAAGAGAGCACCAACGTTGGCCTCTATGAGGGG GACTGGGTTTGGCTGAAGAAGTTCCCAGGGGAACATCATATGGCTATCAGGCCAGCAACAAAGACAGCCT TCTCCAAGCTTCGAGAGCTCCGGCATGAGAATGTGGCTCTCTACTTGGGACTCTTCCTGGCGGGTACAGC AGACAGCCCTGCCACCCCTGGGGAGGGCATCTTGGCTGTGGTCTCAGAGCACTGTGCTCGGGGTTCCCTC CATGACCTCCTGGCCCAGAGAGAAATAAAGCTGGACTGGATGTTCAAGTCTTCCCTCCTGCTGGACCTCA TCAAGGGAATGAGATATCTGCACCATCGCGGTGTGGCCCACGGGAGGCTCAAGTCACGGAATTGCGTGGT GGACGGGAGGTTCGTGCTCAAGGTGACAGATCATGGCCATGGGCGACTGCTGGAAGCGCAAAGGGTGTTA CCGGAACCTCCCAGTGCAGAGGATCAGCTATGGACAGCCCCAGAGCTTCTTCGGGACCCCTCCCTGGAGC GCCGGGGAACTCTAGCTGGTGATGTCTTTAGTCTGGCCATCATCATGCAGGAGGTCGTGTGCCGCAGCAC CCCTTATGCCATGCTGGAACTAACGCCCGAGGAAGTAATACAGAGGGTGCGGAGCCCTCCTCCACTGTGT CGGCCCTTGGTGTCCATGGACCAGGCACCCATGGAGTGCATCCAGCTGATGACACAATGCTGGGCAGAGC ATCCAGAACTTCGGCCTTCCATGGACCTCACCTTTGACCTGTTCAAGAGCATCAACAAGGGCCGGAAGAC CAACATCATCGACTCCATGCTTCGGATGCTGGAGCAGTACTCTAGTAACCTGGAGGATCTGATCCGAGAA CGCACAGAGGAGTTAGAGCAGGAGAAGCAGAAGACAGACAGGCTGCTCACACAGATGCTGCCTCCATCTG TGGCTGAGGCCCTGAAGATGGGGACATCTGTGGAGCCTGAGTACTTTGAAGAGGTGACACTCTACTTCAG TGACATCGTGGGCTTTACCACCATTTCAGCCATGAGCGAGCCTATTGAGGTGGTAGACCTGCTTAATGAC CTCTATACTCTCTTCGATGCCATCATCGGTGCCCATGATGTCTATAAGGTGGAAACAATTGGAGATGCAT ATATGGTGGCCTCCGGGCTGCCGCAGAGGAACGGGCAGCGGCACGCTGCAGAGATTGCCAACATGTCACT GGACATCCTCAGTGCAGTCGGCTCCTTCCGCATGCGCCATATGCCCGAGGTACCGGTGCGCATCCGCATT GGTTTGCACTCAGGCCCGTGCGTGGCGGGTGTGGTGGGCCTCACCATGCCTCGGTACTGCCTGTTCGGGG ACACGGTCAACACTGCCTCGAGAATGGAGTCCACTGGACTGCCTTACCGCATCCACGTTAACATGAGCAC TGTTCGGATTCTTCGCGCTCTGGACCAAGGCTTCCAGATGGAGTGTCGAGGCCGCACGGAGCTGAAGGGC AAGGGTATTGAGGACACGTACTGGCTTGTGGGCAGACTTGGCTTCAACAAGCCCATTCCCAAACCACCTG ATCTGCAGCCAGGGGCCAGCAACCATGGTATCAGCCTGCAGGAGATTCCCCCAGAGAGACGCAAGAAGCT GGAGAAAGCCAGGCCAGGCCAGTTTACTGGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224986 representing NM_008192
Red=Cloning site Green=Tags(s) MSAWLLPAGGLPGAGFCVPARQSPSSFSRVLRWPRPGLPGLLLLLLLPSPSALSAVFKVGVLGPWACDPI FARARPDLAARLAANRLNRDFALDGGPRFEVALLPEPCLTPGSLGAVSSALSRVSGLVGPVNPAACRPAE LLAQEAGVALVPWGCPGTRAAGTTAPAVTPAADALYVLLRAFRWARVALITAPQDLWVEAGRALSTALRA RGLPVALVTSMETSDRSGAREALGRIRDGPRVRVVIMVMHSVLLGGEEQRYLLEAAEELALTDGSLVFLP FDTLHYALSPGPEALAAFVNSSQLRRAHDAVLTLTRRCPPGGSVQDSLRRAQEHQELPLDLNLKQVSPLF GTIYDAVFLLAGGVKRARTAVGGGWVSGASVARQVREAQVSGFCGVLGRTEEPSFVLLDTDASGEQLFAT HLLDPVLGSLRSAGTPMHFPRGGPAPGPDPSCWFDPDVICNGGVEPGLVFVGFLLVIGMGLTGAFLAHYL RHRLLHMQMASGPNKIILTLEDVTFLHPPGGSSRKVVQGSRSSLATRSASDIRSVPSQPQESTNVGLYEG DWVWLKKFPGEHHMAIRPATKTAFSKLRELRHENVALYLGLFLAGTADSPATPGEGILAVVSEHCARGSL HDLLAQREIKLDWMFKSSLLLDLIKGMRYLHHRGVAHGRLKSRNCVVDGRFVLKVTDHGHGRLLEAQRVL PEPPSAEDQLWTAPELLRDPSLERRGTLAGDVFSLAIIMQEVVCRSTPYAMLELTPEEVIQRVRSPPPLC RPLVSMDQAPMECIQLMTQCWAEHPELRPSMDLTFDLFKSINKGRKTNIIDSMLRMLEQYSSNLEDLIRE RTEELEQEKQKTDRLLTQMLPPSVAEALKMGTSVEPEYFEEVTLYFSDIVGFTTISAMSEPIEVVDLLND LYTLFDAIIGAHDVYKVETIGDAYMVASGLPQRNGQRHAAEIANMSLDILSAVGSFRMRHMPEVPVRIRI GLHSGPCVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVNMSTVRILRALDQGFQMECRGRTELKG KGIEDTYWLVGRLGFNKPIPKPPDLQPGASNHGISLQEIPPERRKKLEKARPGQFTGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008192 |
| ORF Size | 3324 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008192.3, NP_032218.2 |
| RefSeq Size | 8331 bp |
| RefSeq ORF | 3327 bp |
| Locus ID | 14919 |
| UniProt ID | P52785 |
| MW | 121.1 kDa |
| Gene Summary | Catalyzes the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors. Plays an essential role in phototransduction, by mediating cGMP replenishment (PubMed:21598940). May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane (PubMed:17255100).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223587 | Gucy2e (untagged) - Mouse guanylate cyclase 2e (Gucy2e), (10ug) |
CNY 14,160.00 |
|
| MG224986 | Gucy2e (tGFP-tagged) - Mouse guanylate cyclase 2e (Gucy2e), (10ug) |
CNY 9,220.00 |
|
| MR224986L3 | Lenti ORF clone of Gucy2e (Myc-DDK-tagged) - Mouse guanylate cyclase 2e (Gucy2e) |
CNY 10,260.00 |
|
| MR224986L4 | Lenti ORF clone of Gucy2e (mGFP-tagged) - Mouse guanylate cyclase 2e (Gucy2e) |
CNY 10,260.00 |
