United States
Germany
Japan
United Kingdom
China
Nlrc3 (NM_001081280) Mouse Tagged Lenti ORF Clone
CAT＃: MR224926L3
- LentiORF®
Lenti ORF clone of Nlrc3 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3)
"NM_001081280" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,260.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLR16.2; D230007K08Rik; mFLJ00348 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR224926).
|
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081280 |
| ORF Size | 3306 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081280.1 |
| RefSeq Size | 4080 bp |
| RefSeq ORF | 3309 bp |
| Locus ID | 268857 |
| Gene Summary | Negative regulator of the innate immune response. Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (PubMed:22863753, PubMed:24560620). May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753). Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (PubMed:27951586). May also affect NOD1- or NOD2-mediated NF-kappa-B activation (By similarity). Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223577 | Nlrc3 (untagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3), (10ug) |
CNY 14,060.00 |
|
| MG224926 | Nlrc3 (tGFP-tagged) - Mouse NLR family CARD domain containing 3 (Nlrc3), (10ug) |
CNY 9,220.00 |
|
| MR224926 | Nlrc3 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3) |
CNY 7,672.00 |
|
| MR224926L4 | Lenti ORF clone of Nlrc3 (mGFP-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3) |
CNY 10,260.00 |
