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Nlrc3 (NM_001081280) Mouse Untagged Clone
CAT＃: MC223577
Nlrc3 (untagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3), (10ug)
CNY 14,060.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CLR16.2; D230007K08Rik; mFLJ00348 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223577 representing NM_001081280
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCGGAGCCCTTTAGCACCCTGGAGCAGCCACCATGGCAGGAAGGAGACAACATAGGTTCCCCAG GGTCAGTCCTGGCTCTGTATTCACAACTTCTGGCTGCAAACACAGACTCCACGAGGAAGCAAGAGGTGTG GACAGACAGAGAGACATGCCTGGCCTACAGTGTTGGCTCCCCAGCTGAGCAGGTGAAAGCCCTTGTGGAT CTGCTGGCTGGGAAGGGCAGTCAGCTGCTACAAGTCCGGGACAAAATGCCAGACTCCCCACTAGGATCCC AGAGCAATGAGTCAAGGATACCGAAGCACTCTGAGGCTCTGCTGAGCAGGGTGGGAAATGACCCAGAACT GGGCAGCCCCTCACACCGGCTGGCCAGCCTCATGCTGGTCGAGGGCCTGACAGACCTGCAGCTAAAGGAG CATGACTTCACACAGGTGGAGGCCACGCGTGGGGTCTGGCACCCTGCCAGAGTTATCACCCTGGACAGGC TCTTCCTGCCTCTGTCCCGGGTATCCATCCCACCTCGAGTCTCTCTCACCATTGGAGTGGCTGGTGTGGG CAAGACCACGCTAGTGAGGCATTTTGTTCATTGCTGGGCCAGAGGACAGGTGGGCAAGGGCTTCTCACGG GTTCTGCCCTTGACCTTTCGGGATCTCAACACCTATGAGAAACTGTCTGCAGACAGACTCATCCAATCCA TCTTCTCAAGCATTGGGGAAGCTAGTCTGGTGGCCACAGCCCCAGACAGAGTCCTCCTGGTCCTGGATGG CTTGGATGAGTGTAAGACACCCCTGGAATTCTCCAATACCATGGCCTGCTCAGACCCAAAGAAGGAGATC CAGGTAGACCACCTGATCACTAACATCATCCGAGGCAACCTCTTTCCAGAAATTTCTGTCTGGATCACCT CCCGGCCCAGTGCTGCTGGTCAGATCCCTGGGGGCCTAGTGGACCGGATGACTGAGATTCGGGGCCTTAC TGAGGAAGAGATCAAAGTGTGTCTGGAGCAGATGTTTCCTGAGGAGCAGAACCTCTTAGGTCAGGTCCTT AGTCAAGTGCAGGCCAACAGGGCTCTGTATCTGATGTGCACTGTACCAGCCTTTTGTAGGCTCACGGGGC TGGCTCTGGGTCACTTGTATCGCACCAGGCTGGCCGTCCAAGACATAGAGCTGCCATTGCCTCAGACCCT GTGTGAGCTCTACTCTTGGTACTTTAGGATGGCTCTTGGTGGGGAGGGCCAGGATAAGGAAAAGGTAAGT CCTAGGATCAAGCAGGTGACCCAGGGAGCTCGCAAAATGGTGGGGACATTGGGCCGCCTGGCCTTCCATG GGCTGGTCAAGAAGAAATACGTGTTTTATGAACAAGACATGAAGGCATTTGGAGTGGACCTCGCTCTGTT GCAGAACACTCTGTGCAGCTGTCTCCTGCAGCGGGAAGAGACCCTGGCCTCCTCTGTAGCTTACTGCTTC ATTCACCTGTCTCTGCAAGAATTTGTGGCAGCTACATATTACTATAGTGCATCCAAGAGGGCCATCTTTG ACCTCTTCACCGAGAGTGGCATGTCCTGGCCCAGACTGGGTTTCCTCGCCCATTTCAGGTGTGCAGCCCA GCGGGCCACACAAGCTAAGGATGGAAGGCTGGATGTGTTTCTGCGCTTCCTCTCTGGCCTCTTGTCCCCA AGGGTCAATACTCTGCTGGCCGGCTCCCTGTTGTCCCAAGGCGAGCATCAGAGCTACCGGGACCAGGTGG CTGAGGTCCTACAAGGCTTCCTTCATCCTGACGCTGCAGTCTGTGCACGTGCCATCAATGTCTTGTACTG CCTAAGTGAGCTGCGGCACACAGAACTGGCCTGCAGTGTGGAGGAGGCCATGCGGAGTGGGACCTTGGCT GGGATGACCAGCCCCTCACACCGCACTGCTCTGGCCTACCTCCTGCAGATGTCTGACATCTGCTCCCCAG AGGCTGACTTCTCCCTGTGTCTCAGCCAGCATGTCCTCCAGAGCCTGCTGCCCCAGCTGCTCTATTGTCA AAGCCTCAGGCTGGACAACAACCAGTTCCAGGACCCTGTGATGGAGTTGCTGGGCAGCGTGCTGAGTGGG AAGGACTGTCGCATTCGAAAGATCAGCCTGGCTGAGAATCAGATTGGTAACAAAGGAGCCAAAGCCCTGG CCAGATCCCTCCTGGTTAACAGAAGCCTCATCACACTGGACCTCCGGAGTAACAGCATTGGACCACCGGG GGCTAAGGCTTTGGCCGATGCTCTGAAGATAAACCGAACGCTAACTTCTCTAAGCCTCCAAAGCAACGTG ATCAAGGATGACGGTGTCATGTGCGTGGCTGAGGCCCTGGTCTCCAACCAGACCATCTCCATGCTACAGC TACAGAAGAACTTAATTGGGCTCATAGGAGCCCAGCAGATGGCAGATGCCCTGAAGCAGAACAGGAGCCT GAAAGCACTCATGTTTTCCAGTAATACCATTGGCGACAGAGGTGCCATAGCCCTGGCTGAGGCCCTGAAG GTGAACCAGATCCTGGAGAACTTAGACCTACAGAGCAATTCCATCAGTGACATGGGAGTGACGGTGCTGA TGCGAGCCCTCTGCAGTAACCAGACACTCTCCAGTCTCAACCTGCGAGAAAACTCCATCAGCCCAGAGGG AGCCCAGGCCCTCACTCAAGCTCTCTGCAGGAACAACACTCTGAAGCACTTGGACCTGACAGCTAATCTC CTCCATGACCGAGGTGCCCAGGCCATTGCAGTAGCTGTGGGAGAAAACCACTCCCTCACACACCTTCACC TGCAGTGGAACTTCATCCAAGCTGGTGCGGCCAGGGCCCTGGGACAAGCACTCCAGCTGAACAGAACCCT GACAACCTTAGACTTACAGGAGAATGCCATAGGGGATGAAGGAGCTTCCTCAGTGGCTGGCGCACTGAAG GTGAACACAACCCTCATTGCTCTCTACCTACAGGTGGCCTCCATTGGTAGCCAAGGGGCCCAGGCACTTG GGGAGGCCCTCACTGTGAACAGAACCTTGGAGATTCTTGACTTACGAGGAAACGACGTTGGGGCAGCTGG AGCCAAGGCCTTGGCAAATGCTTTAAAGTTAAACTCCAGTCTCCGAAGACTCAATCTCCAGGAGAACTCA CTGGGGATGGATGGGGCCATATTTGTTGCCTCTGCACTGTCTGAGAACCACGGTCTGCACCATATTAATC TCCAGGGGAATCCCATTGGGGAGTCTGCTGCCAGGATGATCTCAGAGGCCATCAAGACAAACGCTCCCAC ATGCACTGTGGAAATATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001081280 |
| Insert Size | 3309 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001081280.1, NP_001074749.1 |
| RefSeq Size | 4080 bp |
| RefSeq ORF | 3309 bp |
| Locus ID | 268857 |
| Gene Summary | Negative regulator of the innate immune response. Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (PubMed:22863753, PubMed:24560620). May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753). Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (PubMed:27951586). May also affect NOD1- or NOD2-mediated NF-kappa-B activation (By similarity). Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224926 | Nlrc3 (tGFP-tagged) - Mouse NLR family CARD domain containing 3 (Nlrc3), (10ug) |
CNY 9,220.00 |
|
| MR224926 | Nlrc3 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3) |
CNY 7,672.00 |
|
| MR224926L3 | Lenti ORF clone of Nlrc3 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3) |
CNY 10,260.00 |
|
| MR224926L4 | Lenti ORF clone of Nlrc3 (mGFP-tagged) - Mouse NLR family, CARD domain containing 3 (Nlrc3) |
CNY 10,260.00 |
