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Prdm6 (NM_001033281) Mouse Tagged ORF Clone
CAT＃: MR224755
- TrueORF®
Prdm6 (Myc-DDK-tagged) - Mouse PR domain containing 6 (Prdm6)
ORF Plasmid: tGFP
"NM_001033281" in other vectors (4)
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CNY 6,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm92; PRISM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224755 representing NM_001033281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAAGCCGGGGGATCCCGGCGGCTCGGCCTTCCTCAAAGTGGACCCAGCTTACCTGCAGCACTGGC AGCAACTCTTCCCGCACGGAGGCGGCGGCGGCGGCCCGCTCAAAGCCAGTGGCGCCGCGCTCGCCCTGGG CGCCCCACAGCCCCTGCAGCCACCGCCACCGCCGCCGCCTCCTCCTCCCGAGCGCGCCGAGCCTCCTCCA GACGGCCTGCGGCCGCGGCCTGCCTCGCTCTCGTCCACCCCGGCTCCTTCCTCCACCTCTGCCTCTTCTG CCTCTTCCTGCGCCGCAGCCGCGGCCGCCGCTGCGCTGGCGGGTCTCTCGGCCCTGCCGGTGGCACAGAT GCCGGTGTTCGCTCCTCTAGCTGCTGCTGCTGTGGCCGCGGAGCCACTGCCCCCAAAGGACCTGTGCCTC GGCGCCTCCGCCGGCCCCGGGCCGGCCAAGTGCGGCGGCGGCGGTGGCAGCGTGGGGGACGGCCGTGGCG TCCCACGTTTCCGCTGCAGCGCGGAGGAGCTGGACTATTACCTGTACGGGCAGCAGCGCATGGAAATCAT CCCGCTCAACCAGCACACCAGCGACCCCAACAACCGTTGCGATATGTGCGCGGACAACCGTAACGGGGAG TGCCCCATGCACGGGCCACTGCACTCGCTGCGCCGGCTCGTGGGCACCAGCAGCGCGGCCGCCGCTGCAC CCCCGCCGGAGCTGCCCGAGTGGCTTCGGGATCTGCCGCGGGAGGTGTGCCTGTGTACCAGCACGGTGCC AGGCCTGGCGTATGGCATCTGTGCCGCGCAGAGGATCCAGCAGGGCACCTGGATTGGGCCCTTCCAGGGC GTGCTTCTGTCCCCGGAGAAAGTGCAGACCGGCGTAGTGAGGAACACGCAGCATCTCTGGGAGATATATG ACCAAGATGGGACACTTCAGCACTTTATTGATGGTGGGGAGCCTAGTAAGTCGAGCTGGATGAGGTATAT CCGATGTGCAAGGCACTGTGGAGAACAGAATCTAACAGTAGTTCAGTACAGGTCGAATATATTCTACCGA GCCTGTATAGATATCCCCAGGGGCACCGAGCTCCTGGTGTGGTACAATGACAGCTATACATCTTTCTTTG GGATCCCTTTACAATGCATTGCCCAGGATGAAAACTTGAACGTCCCTTCCACCGTAATGGAAGCCATGTG CCGACAGGATGCCCTGCAGCCCTTCAACAAAAGCAGCAAGCTTTCTCCCTCGGGCCAGCAGCGCTCCGTG GTTTTCCCACAGACTCCCTGCAGCAGGAATTTCTCCCTCTTGGATAAATCTGGGCCCATGGAGGCAGGAT TTAACCAAATCAACGTGAAGAATCAGAGGGTCCTGGCGAGCCCAACCTCAACCAGCCAGCTGCACTCGGA GTTCAGCGACTGGCATCTCTGGAAGTGCGGCCAGTGCTTTAAGACTTTCACGCAGCGCATTCTCCTGCAG ATGCATGTGTGCACGCAGAACCCCGACAGACCCTACCAGTGTGGCCACTGCTCACAGTCCTTTTCCCAGC CGTCAGAACTGAGGAACCACGTAGTCACTCACTCCAGTGACCGGCCTTTCAAGTGCGGCTACTGTGGCCG TGCGTTTGCCGGAGCCACCACCCTCAACAACCACATCCGGACCCACACTGGAGAAAAGCCCTTCAAGTGC GAGAGGTGCGAAAGGAGCTTCACCCAGGCCACGCAGCTGAGTCGACACCAGCGCATGCCCAATGAGTGCA AGCCGATAACCGAGAGCCCGGAATCAATCGAAGTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224755 representing NM_001033281
Red=Cloning site Green=Tags(s) MLKPGDPGGSAFLKVDPAYLQHWQQLFPHGGGGGGPLKASGAALALGAPQPLQPPPPPPPPPPERAEPPP DGLRPRPASLSSTPAPSSTSASSASSCAAAAAAAALAGLSALPVAQMPVFAPLAAAAVAAEPLPPKDLCL GASAGPGPAKCGGGGGSVGDGRGVPRFRCSAEELDYYLYGQQRMEIIPLNQHTSDPNNRCDMCADNRNGE CPMHGPLHSLRRLVGTSSAAAAAPPPELPEWLRDLPREVCLCTSTVPGLAYGICAAQRIQQGTWIGPFQG VLLSPEKVQTGVVRNTQHLWEIYDQDGTLQHFIDGGEPSKSSWMRYIRCARHCGEQNLTVVQYRSNIFYR ACIDIPRGTELLVWYNDSYTSFFGIPLQCIAQDENLNVPSTVMEAMCRQDALQPFNKSSKLSPSGQQRSV VFPQTPCSRNFSLLDKSGPMEAGFNQINVKNQRVLASPTSTSQLHSEFSDWHLWKCGQCFKTFTQRILLQ MHVCTQNPDRPYQCGHCSQSFSQPSELRNHVVTHSSDRPFKCGYCGRAFAGATTLNNHIRTHTGEKPFKC ERCERSFTQATQLSRHQRMPNECKPITESPESIEVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033281 |
| ORF Size | 1788 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033281.3, NP_001028453.1 |
| RefSeq Size | 2601 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 225518 |
| UniProt ID | Q3UZD5 |
| MW | 64.5 kDa |
| Gene Summary | Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression (PubMed:16537907, PubMed:17662997). Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells (PubMed:27181681, PubMed:16537907, PubMed:17662997). Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a (PubMed:16537907). According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression (PubMed:17662997).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219569 | Prdm6 (untagged) - Mouse PR domain containing 6 (Prdm6), (10ug) |
CNY 6,664.00 |
|
| MG224755 | Prdm6 (tGFP-tagged) - Mouse PR domain containing 6 (Prdm6), (10ug) |
CNY 6,560.00 |
|
| MR224755L3 | Lenti ORF clone of Prdm6 (Myc-DDK-tagged) - Mouse PR domain containing 6 (Prdm6) |
CNY 7,890.00 |
|
| MR224755L4 | Lenti ORF clone of Prdm6 (mGFP-tagged) - Mouse PR domain containing 6 (Prdm6) |
CNY 7,890.00 |
