Chrna7 (NM_007390) Mouse Tagged ORF Clone
CAT#: MR224522
- TrueORF®
Chrna7 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7)
ORF Plasmid: tGFP
"NM_007390" in other vectors (4)
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CNY 3,736.00
CNY 4,370.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Acra7; alpha7; nAchR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224522 representing NM_007390
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGGCCGGCGGGGAGGCATCTGGCTGGCTCTGGCCGCGGCGCTGCTGCACGTGTCCCTGCAAGGCG AGTTCCAGAGGAGGCTGTACAAGGAGCTGGTCAAGAACTACAACCCGCTGGAGAGGCCGGTGGCCAACGA CTCGCAGCCGCTCACCGTGTACTTCTCCCTGAGCCTCCTGCAGATCATGGATGTGGATGAGAAGAACCAA GTTTTAACCACCAACATTTGGCTACAAATGTCTTGGACAGATCACTATTTGCAGTGGAACATGTCTGAGT ACCCCGGAGTGAAAAATGTTCGTTTTCCAGATGGCCAGATTTGGAAACCAGACATTCTCCTCTATAACAG TGCAGATGAACGCTTTGATGCCACATTCCACACCAACGTCTTGGTGAATGCATCTGGGCATTGCCAGTAT CTCCCTCCAGGCATATTCAAGAGCTCCTGCTACATCGATGTACGCTGGTTCCCTTTTGATGTGCAGCAGT GCAAACTGAAGTTTGGGTCCTGGTCCTATGGAGGGTGGTCCTTGGACCTGCAGATGCAAGAGGCAGATAT CAGCAGCTATATCCCCAATGGAGAATGGGATCTCATGGGAATCCCTGGCAAAAGGAATGAGAAGTTCTAT GAATGCTGCAAAGAGCCATACCCAGATGTCACCTACACAGTAACCATGCGCCGTAGGACACTCTACTATG GCCTCAACCTGCTCATTCCTTGTGTGCTCATTTCAGCCCTGGCTTTGCTGGTATTCTTGCTGCCTGCAGA CTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGACTGTCTTCATGCTGCTTGTGGCT GAGATCATGCCAGCAACATCTGATTCCGTGCCCTTGATAGCACAGTACTTCGCCAGCACCATGATCATCG TGGGCCTCTCAGTGGTCGTGACAGTGATTGTGCTGCGATATCACCACCATGACCCTGATGGTGGCAAAAT GCCTAAGTGGACCAGGATCATTCTTCTGAATTGGTGTGCATGGTTTCTGCGCATGAAGAGGCCGGGAGAG GACAAGGTGCGGCCGGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCTGGCCAGTGTGGAGCTGAGCGCAG GTGCGGGGCCACCCACCAGCAATGGCAACCTGCTCTACATTGGCTTCCGAGGCCTGGAGGGCATGCACTG TGCCCCAACTCCAGACTCTGGGGTCGTGTGTGGTCGTTTGGCCTGCTCCCCAACACATGATGAGCACCTC ATGCATGGTACACACCCCTCTGATGGGGACCCCGACCTGGCCAAGATCCTGGAGGAGGTCCGCTACATCG CCAACCGCTTCCGCTGCCAGGATGAGAGTGAGGTGATCTGCAGTGAGTGGAAGTTTGCGGCCTGTGTGGT GGACCGCTTGTGCCTCATGGCCTTTTCGGTTTTTACCATCATCTGCACCATCGGCATCCTCATGTCAGCC CCAAACTTTGTGGAGGCTGTGTCCAAAGACTTTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224522 representing NM_007390
Red=Cloning site Green=Tags(s) MCGRRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQ VLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQY LPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFY ECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVA EIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGE DKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHL MHGTHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSA PNFVEAVSKDFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007390 |
ORF Size | 1506 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007390.3, NP_031416.3 |
RefSeq Size | 2091 bp |
RefSeq ORF | 1509 bp |
Locus ID | 11441 |
UniProt ID | P49582 |
MW | 57.1 kDa |
Gene Summary | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Activation of the α7 Nicotinic Acetylcholine Receptor Prevents against Microglial-Induced Inflammation and Insulin Resistance in Hypothalamic Neuronal Cells
,null,
Cells
,PubMed ID 35883638
[Chrna7]
|
Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?
,Deshpande, A;Vinayakamoorthy, RM;Garg, BK;Thummapudi, JP;Oza, G;Adhikari, K;Agarwal, A;Dalvi, P;Iyer, S;Thulasi Raman, S;Ramesh, V;Rameshbabu, A;Rezvaya, A;Sukumaran, S;Swaminathan, S;Tilak, B;Wang, Z;Tran, PV;Loring, RH;,
Biomolecules
,PubMed ID 32204458
[CHRNA7]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216944 | Chrna7 (untagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7), (10ug) |
CNY 3,744.00 |
|
MG224522 | Chrna7 (tGFP-tagged) - Mouse cholinergic receptor nicotinic alpha polypeptide 7 (Chrna7), (10ug) |
CNY 5,336.00 |
|
MR224522L3 | Lenti ORF clone of Chrna7 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7) |
CNY 6,270.00 |
|
MR224522L4 | Lenti ORF clone of Chrna7 (mGFP-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7) |
CNY 6,270.00 |